Citations

Induction of EAE:

Induction of MOG EAE was done as previously described.[29, 30] Shortly, 8 to 9-week-old female C57BL/6 mice were immunized with an emulsion containing 300 µg of MOG35-55 in phosphate-buffered saline and an equal volume of complete Freund’s adjuvant containing 5 mg/mL H37RA (Difco Laboratories, Detroit, MI). The inoculum (0.2 mL) was injected subcutaneously into the left flank. One hundred nanograms of pertussis toxin (List Biological Labs, Campbell, CA) in 0.1 mL phosphate-buffered saline was also injected i.p. on day 0 and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

Pertussis toxin (200 ng per mouse; List Biological Laboratories, Campbell, CA, USA) was injected intraperitoneally on the day of immunization (day 0) and again 2 days later. Mice were monitored daily for clinical signs and scored as follows: 0, no disease; 1, tail paralysis; 2, paraparesis; 3, paraplegia; 4, paraplegia with forelimb weakness or paralysis; 5, moribund or dead animals.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…Pertussis toxin (List Biological Laboratories, …was given at 200 ng per mouse on the day of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

CNS inflammation was induced using a well-established model of EAE…In brief, groups of 10-wk-old age- and sex-matched mice were s.c. injected in the flank with 100 mg MOG₃₅₅₅ peptide (Genemed)… The same day and 2 d later, 200 ng pertussis toxin (List Biological Labs) in 200 µl physiological saline was injected i.v.

For Foxp3^DTR/+ mice, 8-wk-old females were injected i.p. on days 10, 7, and 4 with 500 ng diphtheria toxin (List Biological Labs). Then EAE was induced as described above.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Animals. Induction and treatment of EAE:

…C57BL/6 mice were bred and maintained in the facilities of the Forschungsinstitut fr Experimentelle Medizin (FEM, CharitUniversittsmedizin, Berlin, Germany), under specific pathogen-free (SPF) conditions. EAE was induced as described previously… 200 ng of Bordetella pertussis toxin (List Biological Laboratories, Campell, CA) was administered intraperitoneally at day 0 and 2. EAE experiments were carried out in our animal facilities under standard husbandry conditions. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Antibodies and Regents:

…Cholera toxin B subunit-biotin conjugate reacting with GM1 is from List Biological Labs (Campbell, CA). …

• Product #112 – Cholera Toxin B Subunit Biotin Conjugate

Conditioning and Dorsal Column Lesions:

The right sciatic nerve of both WT and MT-I/II-deficient mice (n = 6 for both groups) was lesioned. 7 days later under isoflurane anesthesia, a laminectomy was performed at the T89 level, and the dorsal column was transected to a depth of 1 mm. Another set of WT and MT-I/II-deficient mice (n = 6 for both groups) received only dorsal column lesions. At 5 weeks after surgery, 2 l of 1% cholera toxin B (CTB; List Biological) was injected into the right sciatic nerve, and the animals were transcardially perfused with 4% (PFA) 3 days later. Spinal cord sections were immunostained using goat anti-CTB antibody (1:2000; List Biological), …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Induction of EAE:

DR*1501-Tg and DR*1502-Tg mice were screened for the expression of the HLA marker by flow cytometry [30]. Mice between 8 and 12 weeks of age were immunized s.c. at four sites on the flanks with 0.2 ml of an emulsion of 200 µg immunogenic peptide and complete Freunds adjuvant containing 400 µg of heat-killed Mycobacterium tuberculosis H37RA [30] (Difco, Detroit, MI). In addition, mice were given Pertussis toxin (Ptx) from List Biological Laboratories (Campbell, CA) on days 0 and 2 post-immunization (75 and 200 ng per mouse, respectively). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

C57BL/6 WT and Dbc1^/ mice were injected s.c. with 100 mg of an emulsion of MOG35-55 peptide …Pertussis toxin (List Biological Laboratories) at a dose of 250 µg per mouse was injected i.v. on days 0, 2, 7, and 9.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Mice were vaccinated subcutaneously in both flanks with 50 µl into each side. All animals received an ip dose of 200 ng of pertussis toxin (List Biologicals, Campbell, CA) in a total volume of 100 µl on the day of immunization and 2 days later. Scoring of EAE …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)