Bacterial Toxin Research Citations

… 100ng/mL cholera toxin (List Biological Laboratories, 100B)). Cell lines were maintained at 37°C in a 5% CO2 cell culture incubator and passaged 556 at 70-90% confluency. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… At days 0 and 2 post-immunization (PI), pertussis toxin (500 ng; List Biological Laboratories, Inc., Campbell, CA) was injected intraperitoneally …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell Culture:

… MCF 10A cells (ATCC, USA) were cultured in mammary epithelial growth medium (MEGM; Lonza, Switzerland) supplemented with 100 ng/ml of cholera toxins (List Biological Laboratories, USA) and 1 % (v/v) A/A. All of the cells were cultured in a humidified incubator of 37 ℃ and 5 % CO2. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Mice were intraperitoneally 10 injected with 200 ng of pertussis toxin (PTX) (List Biological Labs, Inc., Campbell, CA, 11 USA) in PBS on days 0 and 2. Clinical EAE scores were monitored for 30 days according 12 to the following grades: 0, no disease; 0.5, partially limp tail; 1, paralyzed tail; 1.5 …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… After acclimating to the environment for one week, mice were subcutaneously injected with MOG35-55 (200 µL, 1 mg/mL). Pertussis toxin (LOT: 180244A1; List Biological Laboratories, CA, USA) was dissolved in physiological saline (200 ng/100 µL). …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

EAE induction and MOG restimulation

Mice used in each experiment were 4- to 12-month-old female littermates. Experimental autoimmune encephalomyelitis (EAE) was induced by subcutaneous injection of mice (four per group) with 200 μg myelin oligodendrocyte glycoprotein (MOG) peptides (amino acids 35–55: MEVGWYRSPFSRVVHLYRNGK; Genemed Synthesis)54 emulsified in CFA (Chondrex). Mice were also intraperitoneally injected with 200 ng pertussis toxin (List Biological Laboratories) on days 0, 1, and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

4.2. Mice and EAE induction

EAE was induced 4 weeks following vector administration in female C57BL/6 mice (The Jackson Laboratory, Bar Harbor, ME, USA). Briefly MOG35-55 (200ug) emulsified in Complete Freund’s Adjuvant (Sigma Aldrich, St. Louis, MO) with 9 mg/mL Mycobacterium tuberculosis (Difco Laboratories, Franklin Lakes, NJ) was injected subcutaneously over two sites at the upper and lower back. 2- and 24- hours following immunization, 200 ng Pertussis Toxin (List Biological Laboratories, Inc, Campbell, CA) was injected interperitoneally. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… In TeNT treatment, an 8-nl mixture containing 0.2M KCL, 0.5% dextran Antonia Red 150,000 MW (Sigma) and TeNT (List Labs, 190A; 0.5M) was pressure injected into the hindbrain ventricle. Between 50 and 70 min after injection, dextran was imaged, followed by another 10 min of GCaMP6s imaging. …

• Product #190 – Tetanus Toxin from Clostridium tetani

… Three days before euthanasia, rats were anesthetized with isoflurane (5% for induction; 2-2.5% for maintenance), and 15-20 µL cholera toxin B subunit (CTB, #104 List Biological Labs, Campbell, California, USA) was bilaterally injected into the intrapleural space to retrogradely label PhMNs, as previously described (Cheng et al 2021a) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt