Bacterial Toxin Research Citations

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Pertussis toxin (PTX, cat: 181) was purchased from List Biological Laboratories (CA, USA). …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

2.2. Experimental design

… The two experimental sessions were held between two and four weeks apart to mitigate potential carry-over effects from LPS-mediated immune activation. …

The LPS infusion was prepared on-site immediately before administration, following manufacturer protocols for reconstitution and dilution under sterile conditions. In short, the product was reconstituted and initially diluted with sterile water, then diluted to the final dose in 30 mL sterile saline within 15 min of administration. For each participant, a single fresh vial of Clinical Center Reference lyophilized endotoxin (lipopolysaccharide/LPS; lot 94332B1) was used, prepared from Escherichia coli O113:H10:K by List Biological Laboratories, Inc. The infusion was administered at a slow rate of 1 mL/min over 30 min using an infusion pump (Taudorf et al., 2007). In the first session, LPS was administered at a very low dosage of 0.1 ng/kg of participant body weight, and in the second session at a low dosage of 0.4 ng/kg. …

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113

smFISH and immunostaining

… For immunostaining, sections were permeabilized in PBS + 0.3% Triton X-100, blocked for 1 h in block buffer (PBS + 0.3% Triton + 10% normal donkey serum) and incubated with primary antibodies in block buffer at 4 °C overnight. Slides were washed three times, incubated in secondary antibodies in block buffer for 1 h at room temperature and washed three times, and a coverslip was applied with ProLong Gold Antifade Reagent. Primary antibodies included: chicken anti-GFP (Aves Labs, GFP-1010, 1:1,000), rabbit anti-c-Fos (Synaptic Systems, 226 003, 1:5,000), goat anti-ChAT (Millipore, AB144P, 1:100) and goat anti-CTB (List Labs, 703, 1:1,000). Species-specific donkey secondary antibodies conjugated to Alexa Fluor 488, 568 or 647 were obtained from Life Technologies or Jackson ImmunoResearch and used at a 1:500 dilution. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Animals were given two 400 ng intraperitoneal injections of B. pertussis toxin (Cat# 181; List Biological Laboratories) on days 0 and 2 …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

2.2 Drugs

… Pertussis toxin (PTX) (180243A1) was purchased from List Biological Laboratories (Campbell, USA). …

2.3 Induction and grouping of EAE

Twenty healthy C57BL/6 female mice were randomly divided into four groups: control group (n = 5), EAE model group (n = 5), EAE+BAI treatment
group (n = 5) and the EAE+DEX treatment group (n = 5). The mice were immunized with a mixture of MOG35-55 peptide (200 μg) and CFA, which was
ultrasonically emulsified. On the day of immunization, each EAE mouse received intraperitoneal injections of PTX diluent (0.1 ml, containing 250 ng) on Day 0 and Day 2, while the control mice received an equivalent volume of saline administered intraperitoneally (Figure 1B).

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… 5 mg/mL heat-killed 493 Mycobacterium tuberculosis (Cat#231141, BD Bioscience), followed by a tail vein injection of 200 ng of pertussis toxin (Cat#179B, List Biological Laboratories) on day 0. Intraperitoneal injection of 200 ng of pertussis toxin was administered on day 2 …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… DT, unnicked (List Biological Laboratories), was injected intraperitoneally at a dose of 20 ng per g of body weight in 1 × PBS for initial depletion and phenotyping. Where applicable, DT at a dose of 4 ng per g of body weight in 1 × PBS was then injected intraperitoneally every 3 d for continuous depletion. We used 1 × PBS alone as a vehicle control. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae