Bacterial Toxin Research Citations

Immunohistochemical Methods:

Immunocytochemical detection was performed by standard light, fluorescent microscopy…Rats were perfused transcardially (4% paraformaldehyde solution). Sections were incubated (overnight/4C)…goat anti CTb primary antibody (1/10,000; List Biological Laboratories).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE induction and disease scores:

EAE was induced in C57BL/6 (The Jackson Laboratory, Bar Harbor, ME) female mice (8-10 weeks old). …Pertussis toxin (List Biological Labs Inc., Campbell, CA) was administered by intraperitoneal (ip) injection at day 0 (75 ng per mouse) and day 2 (200 ng per mouse) after immunization. Mice were scored daily for EAE …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis:

… For active EAE, Sort1 / and WT littermate mice were immunized sc with 250 µg MOG 3555 or whole-protein MOG…and received 200 ng pertussis toxin (List Biological Laboratories) ip at the time of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tracers and coordinates:

Retrograde tracing was accomplished using 3-10nl injections of Cholera toxin subunit B (CTb) (10 µg/µl saline, List Biological, Campbell, CA), whereas anterograde tracing was done using either 3-15 nl injections of biotinylated dextran amine (BDA) …

<• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Recovery Surgical Procedures: Retrograde Tracer Injections and Catheters (Studies 2 and 3):

…In rats to be used in study 2, one of the following retrograde tracers was microinjected into the left dorsal lateral column containing preganglionic sympathetic fibers: Fluoro-Gold (FG; 2% in deionized water; Fluorochrome, Denver, CO; n = 2), cholera toxin subunit (CTB; 1% in deionized water; List Biological Laboratories, Campbell, CA; n = 7), …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… For optimum visualization of CTb, sections were incubated overnight at RT with a goat anti-CTb antibody (1:10,000 List Biological Laboratories, CA, USA) diluted in 0.1 M PBS containing 0.3 % Triton X-100 and 0.5 % BSA. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Induction of EAE:

Induction of myelin oligodendrocyte glycoprotein (MOG) EAE was done as previously described.30,31 Shortly, 6- to 8-week-old female C57BL/6 mice were immunized with an emulsion containing 200 µg of MOG_3555…The inoculum (0.2 mL) was injected subcutaneously into right and left flanks. One hundred nanograms of pertussis toxin (List Biological Labs, Campbell, CA, USA) in 0.1 mL saline was also injected intraperitoneally on day 0 and 48 hours later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Analysis of TMV-PA Antigens for Reactivity against PA Specific Antibodies:

SDS-PAGE, ELISA and Western Blots – 
 
… For westerns, gels were transferred onto 0.2 um nitrocellulose
membranes (7 cm x 8.5 cm) in Tris-Glycine buffer (25 mM Tris, 192 mM Glycine, 20%
Methanol, pH 8.3) for 1 hour at 100V. Membranes were blocked with 2.5% non-fat milk
in TBS (20 mM Tris, 150 mM NaCl, pH 7.6) for 1 hour at room temperature. Primary
antibody (Goat anti-PA polyclonal, List Biologics Lot # 7712A2 …

… (c) Wash sections with 0.01 M PB three times for 5 min each time. (d) Bathe sections in primary anti-CTB antibody, for example, List (List Biological Lab) goat anti-CTB (1:5005,000) in 0.01 M PB containing 1 % BSA and 1 % TX-100 either overnight at 4 C or 35 h at RT. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Induction of Active EAE in Mice by Immunization with MOG and Adjuvant:

EAE was induced in female wild-type C57BL/6 mice or MT and IL-10deficient mice on C57BL/6 background…All mice were given 400 ng of pertussis toxin (List Biological) intraperitoneally at 0- and 48-h postimmunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)