Citations

Materials and Methods:

… Pertussis toxin (PTX) was from List Biologicals (Campbell, USA) (stock solution: 0.5 µg/µL in water), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice were injected in both thigh muscles with 50 L of a formulation containing…or 20 g/mL anthrax recombinant protective antigen (rPA) (List Biological Laboratories, Campbell, CA)…

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Lung culture, drug treatments and imaging for morphometry:

… Lung cultures were treated with the following additions to the media to achieve final concentrations as follows: …botulinum toxin, serotype A (BTx): 50, 100, or 200 ng/mL (List Biologicals #128A, Campbell, California, USA). BTx was stored at 4°C (in a locked refrigerator), reconstituted at 100 ng/µL in water with 1 mg/mL BSA and handled in a fume hood. …

Neuronal tracing of afferent projections from C2 nerve:

Adult male Wistar rats (250 g, n = 5) were anaesthetised with 5 % isoflurane in O₂. A dorsal midline incision was then made from the base of the skull to the scapula. …Tissue was then sectioned coronally at 50 m and washed three times in PBS before incubation in goat anti CTb (1:10,000 in PBS containing 0.1 % triton X-100, List Biological) overnight at 4 C. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE induction:

… In addition, the mice received 400 ng pertussis toxin (List Biological Laboratories) intraperitoneally (i.p.) on days 0 and 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animals and induction of EAE:

… A second injection of MOG emulsion was injected in the left flank one week later. In addition, animals received an intraperitoneal injection of 500 ng pertussis toxin (List Biological Laboratories, Campbell, CA) dissolved in 0.2 mL PBS on days 0 and 2 of the study. Normal control mice were anesthetized and received sterile PBS injections of equal volume in place of MOG and pertussis immunizations. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Generation and polarization of macrophages:

…M0 macrophages were also similarly generated from fresh monocytes isolated from PBMCs by the Percoll-based method. For M1 or M2 macrophage polarization, M0 cells were treated for 2 days with 20 ng ml 1 recombinant human IFN g (Life Technologies) and 100 ng ml 1 Escherichia coli LPS (List Biological Laboratories, Campbell, CA), or 20 ng ml 1 recombinant human interleukin 4 (Life Technologies), respectively.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Eight-week-old mice were infected with 102 PFU of WNV or diluent (mock), and BBB permeability was assessed after 4 days. In other experiments, mice were treated with LPS (List Biological Laboratories, 3 mg/kg via intraperitoneal injection)…

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

EAE Model:

Mice also received 200 ng of pertussis toxin (List Biological Laboratories) by intraperitoneal injection on days 0 and 2. Classical disease severity was assessed daily by assigning clin- ical scores according to the following ascending paralysis scale: 0, no disease; 1, tail paralysis; 2, weakness of hind limbs; 3

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Methods:

Recombinant murine prolactin was tested in virgin C57BL/6 female mice aged 8 to 10 weeks. EAE was induced at day 0 by 50 µg myelin oligodendrocyte glycoprotein (MOG), peptide 35-55, …Mice were also administered two intraperitoneal injections of pertussis toxin (List Biological Labs, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)