Bacterial Toxin Research Citations

-Galactosidase and CT assays:

… A positive-control assay for quantification of the level of CT in the samples was performed using purified CT (List Biological Laboratories).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materials and Methods:

…CTB was visualized by incubating sections in a goat anti-CTB polyclonal antibody (List Biological Laboratories, 1 : 33 000 dilution) overnight, …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… in addition, there are several versions of FRET based peptides, for example, VAMPtide (O-Abz/Dnp), VAMPtide (FITC/DABCYL), … available on market (List Biological Laboratories, INC, USA)

• Product #540 – VAMPtide Peptide Substrate (o-Abz/Dnp) Substrate for C. botulinum Type B Neurotoxin
• Product #541 – VAMPtide Peptide Substrate (FITC/DABCYL) Substrate for C. botulinum Type B Neurotoxin

Induction of EAE and clinical evaluation:

An emulsion (0.2 ml) was prepared by mixing 0.1 ml of 200 µg PLP in phosphate-buffered saline (PBS) solution and 0.1 ml of complete Freunds adjuvant (CFA) containing killed mycobacterium tuberculosis strain H37RA (Difco, Detroit, MI) with a final concentration of 4 mg/ ml. On day 0, the emulsion was subcutaneously (s.c.) injected into SJL/J female mice (57 weeks old) at regions above the shoulder and the flanks (total of 4 sites; 50 µl at each injection site). Then, 200 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) was injected intraperitoneally (i.p.) on the day of immunization (day 0) and 48 h post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

C57BL/6J mice were injected SC with 50 µg MOG p35-55 (Auspep, Parkville, Australia) emulsified in complete Freund’s adjuvant containing 200 µg heat-killed Mycobacterium tuberculosis on day 0. Mice received 200 ng Bordetella pertussis toxin (List Biological Laboratories, Campbell, CA) IV in 0.2 mL phosphate-buffered saline (PBS) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Membrane Potential Assay: Pertussis Toxin (PTX) Pretreatment (n= 3):

AtT20-hCB1 or AtT20-hCB2 cells were plated at 81 µL in even rows and 90 µL in
odd rows of a 96 well plate. 9 µL of 2 µg/mL PTX (List Biological Laboratories, Campbell,
California, USA) was added to each well in the even rows making the final concentration of
PTX 200 ng/mL per well. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… mg/ml Mycobacterium tuberculosis H37Ra (Difco). Two hundred nanograms of pertussis toxin (List Biological Lab, Epsom, England) was given ip on days 0 and 2 post-immunization (pi). Mice were scored daily for appearance …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunization for EAE induction and NSC/HSPC injection:

C57BL/6 NSCs or C57BL/6 HSPCs (Lineage^negC-kit^pos) were either treated with GPS or not, and 1 10⁶ cells were injected into the tail-vein of C57BL/6 mice on Days 9 and 13 post immunization (PI) with MOG 35-55. MOG 35-55 (M-E-V-G-W-Y-R-S-P-F-S-R-O-V-H-L-Y-R-N-G-K) corresponding to mouse sequence is synthesized by …and injected intraperitoneally with 200 ng Pertussis toxin (List Laboratories, Campbell, CA) on the day of immunization and 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Pertussis toxin treatment

…we tested different doses of PTx, including 100, 200, 400 and 1000 ng; and different route of administration including intracerebroventricular, intravenous, and i.p. injections. The results showed 1000 ng had the most efficacy without toxicity and i.p. was the most convenient route without irritation. Thirty minutes after inducing pMCAO, we injected either PTx (List Biological laboratories Inc.) i.p. (1000 ng in 1.0 mL phosphate-buffered saline) in the treatment group or the same volume phosphate-buffered saline (1.0 mL) in the control group.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

…and pertussis toxin from List Biological Laboratories (Campbell, California, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)