Bacterial Toxin Research Citations

Dorsal column injury:

Three days before perfusion, animals (Fisher rats) in the 8-week time point after dorsal root injury and in the dorsal column injury group received 3 µl of 1% cholera toxin B (CTB) (103B, List Laboratories, Inc., Campbell, CA) into the sciatic nerve to transganglionically label dorsal root and dorsal column axons.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Spinal Cord Injections:

Layer V corticospinal motor neurons were labeled by stereotaxic injection of cholera toxin B subunit (CTB), a retrograde neuronal tracer, into the cervical enlargement at C5 of 5 month-old CD1 mice….and 500 nL of CTB tracer (6.67 g per l, List Biological Labs) was injected into the right dorsal corticospinal tract (dCST)⁵⁴. CTB infusion progressed at 0.2 mL per min through a glass micropipette connected by special adapters and high pressure tubing to a 10 mL Hamilton syringe….Cervical cord was cryosectioned at 40m and for dCST injection site verification by CTB immunofluorescence (Goat anti-CTB, List Biological Labs Product #703).

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE Induction and Treatment:

Mice were immunized s.c. for active induction of EAE as described… Two hundred nanograms of pertussis toxin (List Biological Lab, …were given intraperitoneally on days 0 and 2 post-immunization (p.i.).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…Western blot analysis was performed as in [24] with the following modifications. Primary antibodies were diluted in blocking buffer at 1:1000 (Goat anti-PA polyclonal, List Biological Laboratories) or…

Reagents:

Anthrax Lethal Factor and Protective Antigen were purchased from List Biological Laboratories, Inc.

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Ultra Pure LPS (Escherichia coli 0111:B4, LIST Biological Laboratories, Campbell, Calif., USA) was dissolved in sterile normal saline and injected intraperitoneally (1 mg/kg) every 2 days to induce chronic endotoxemia (n = 9 for each designated time point). …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Murine EAE model:

EAE was induced in female WT and Abcc8/ mice at 8 weeks of age, …Each mouse then received 400 ng of pertussis toxin (List Biological Laboratories) intraperitoneally (IP) on post-induction day (pid) 0 and pid-2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… LPS was obtained from List Biological Laboratories, Inc (Campbell, CA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Immunizations and Infections:

For Treg ablation experiments, Foxp3-DEREG mice were injected intraperitoneally with 75 g/kg diphtheria toxin (List Biologicals) at days 1 and 2 post antigen immunization.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Reagent:  Cholera toxin B subunit (CTB) was from List Biological Laboratories.

Macrophage Culture, Transfection, Infection, and Cytotoxicity Assay:

For CTB treatment, primed cells were stimulated with 20 µg/ml CTB plus 1 µg/ml LPS (Kayagaki et al., 2011 and 2013).

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt