Bacterial Toxin Research Citations

Cell culture:

Keratinocyte culture medium (KCM) was composed of a basal mixture of 3 parts DMEM to 1 part nutrient mixture F-12 Ham ), …1 nM cholera toxin (List Biological Labs, Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Recombinant proteins and reagents:

Protective antigen (PA) and edema factor (EF) were purchased from List Biological. …

Cell seeding and incubation with toxins:  

For toxin treatment, patterned and nonpatterned peritoneal macrophages and BMDMs were incubated with PA at 1 g/ml and EF at 100 ng/ml for 6 or 16 h at 37C in a 5% CO₂ atmosphere. For some experiments, cells were incubated with PA alone at 1 g/ml.

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

… Two adjuvant molecules were used: poly (I:C) (Invivogen, San Diego, CA) administered via the intravenous route (25 µg per mouse) and cholera toxin (CT, List Biological Laboratories, Campbell, CA) injected via the intradermal route (1 µg per mouse). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

2.1. Materials:

… Cholera toxin (CT) was from List Biological Laboratories, Inc. (Campbell, CA, USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… In the experiments with LPS, animals (n = 36) received intraperitoneal (ip) saline-based injections of ultra-pure LPS (lot # 4231A1; List Biological Laboratories, Campbell, CA, USA) in the dose of 1 mg kg 1 ….

• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5

Treatment conditions:

… To determine the effect of MPLA exposure on LPS-mediated cytokine activation, HUVECs were exposed to 10 g/ml of MPLA and co-incubated in fresh media containing 0% or 2% FBS with Ultra-Pure LPS from E. coli 0111:B4 (100 ng/ml; List Biological Laboratories, Inc. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Reagents:

Ultrapurified Escherichia coli (E. coli) 0111:B4 LPS was purchased from List Biologicals Laboratory, Inc. (Campbell, CA). …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

EAE:

…Pertussis toxin (300 ng in PBS; List Biological, USA) was injected intraperitoneally at the time of induction and a second dose was administered three days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animal Models (Cell depletion):

The CD11b⁺ cells were depleted in CD11b-diphtheria toxin receptor (DTR) mice (congenic in the C57BL/6 background) by intraperitoneal injection with diphtheria toxin (DT; 7.5 ng/g body weight; List Biological Laboratories) or phosphate-buffered saline (PBS) on days 7 and 9, with sacrifice on day 10. We gave DT twice as continued DT treatment is toxic and can lead to saturation of the DTR. Effects of CD11b⁺ cell depletion were assessed on day 8 and day 10 (1 day after treatment) as CD11b⁺ cells repopulate the liver 48 hours after DT depletion.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

T cell activation for phenotyping experiments:

… As a positive control, PBMCs also stimulated with staphylococcal enterotoxin B (List Biologicals) at 5 µg/ml.