Bacterial Toxin Research Citations

Hormone treatment and induction of EAE:

… Mice received pertussis toxin through intraperitoneal injection (Ptx, List Biologicals, Campbell, CA) on the day of immunization (75ng) and 2 days later (200ng). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Mice received intraperitoneal injections of 400 ng of pertussis toxin (List Biological Laboratories, Campbell, California) the same day and again 48 h later. The animals were monitored daily …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animal Model of EAE and Clinical Evaluation:

…Briefly, SJL mice were immunized subcutaneously at the tail base with 100 µl of peptide emulsion containing 150 µg PLP₁₃₉₁₅₁ antigen and an equal volume of complete Freunds adjuvant supplemented with 200 µg Mycobacterium tuberculosis H37Ra. C57BL/6 mice were immunized subcutaneously at the tail base with 150 µg MOG peptide₃₅₅₅ dissolved in distilled water and emulsified with an equal volume of complete Freunds adjuvant supplemented with 4 mg/ml M. tuberculosis H37Ra. Immediately after immunization, animals received an intravenous injection of 200 ng Bordetella pertussis toxin (List Biological Laboratories, Campbell, CA, USA) in 200 µl PBS. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… killed Mycobacterium tuberculosis strain H37RA (Difco). Along with each immunization, 200 ng Bordetella pertussis toxin (List Biological Laboratories) was administered by intraperitoneal injection. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

Subsequently, the mice received intraperitoneal injection of 200 ng of pertussis toxin (List Biological Laboratories) on days 0 and 2. Disease states of EAE were assessed and given the following scores: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

pertussis toxin (PTX) from List Biological laboratories (Cambell, CA …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

… On day 0, mice received a subcutaneous injection of 150µg emulsified in incomplete Freunds adjuvant containing 500g heat-killed Mycobacterium tuberculosis (Difco, Detroit, MI). Also on day 0 and again on day 2, mice received an intraperitoneal injection of 200ng pertussis toxin (List Biological Laboratories, Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… DT (6.25 ng/g, IP, List Biological Laboratories) was injected at either PI or P6 into Pou4f3Dm- mice. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Reagents:

Cholera enterotoxin was from List Biologicals (Campbell, CA, USA).

Tissue culture:

MCF-10A cells were maintained in DMEM/F12 (1:1) medium supplemented with 5% horse serum, 10 µg/ml insulin, 10 ng/ml EGF, 0.5 µg/ml hydrocortisone and 100 ng/ml Cholera toxin.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Lipid Analyses and Visualization:

T…  Cell surface GM1 levels were examined by flow cytometry. Cells were detached by trypsin and incubated with 1 µg/ml biotin-conjugated cholera toxin B subunit (biotin-CTxB) (List Biological Laboratories) for 60 min at 4°C. …

Immunofluorescence Microscopy:

Cells were seeded on glass coverslips in 6-well plates and grown for 2 days in medium A. After incubation of the cells in medium F overnight, they were fixed with 4% paraformaldehyde for 10 min and blocked with 5% FBS in PBS for 1 h. Specimens were incubated with biotin-conjugated CTxB (1 µg/ml) and anti-caveolin-1 antibody or with biotin-conjugated CTxB (1 µg/ml) and anti-flotillin-1 antibody for 1 h at room temperature. …

• Product #112 – Cholera Toxin B Subunit Biotin Conjugate