Bacterial Toxin Research Citations

CCR2⁺ monocyte depletion:

CCR2⁺ monocytes were depleted in CCR2-DTR mice and control CCR2-DTR negative littermates by i.p. administration of 250 ng of diphtheria toxin 2.5 days post CPS vaccination…Diphtheria Toxin was purchased from List Biological Laboratories (Campbell, CA), and reconstituted at 1 mg/ml in PBS. Aliquots were stored in 80C. Spleens were harvested on D5 post CPS vaccination, and tgd057-specific CD8⁺ T cells were analyzed.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Evidenciado a completa anestesia do animal, eles foram submetidos a uma injeo intra-ocular unilateral de 80 µl de uma soluo aquosa do traador neuronal antergrado, a CTb (CTb, List Biological L aboratories, Inc., Campbell, CA ) a 0,1%, contendo dimetilsulfxido a 10 % para aumentar a permeabilidade e consequentemente, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Cells were treated with dimethyl sulfoxide at a final concentration of 0.5% and used as a vehicle control. Purified LPS from Escherichia coli was purchased from List Biological Laboratory Inc. (Campbell, CA, USA) and dissolved in endotoxin-free water. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Diphtheria Toxin Injections:

Diphtheria toxin (DT) (List Biologicals) was diluted in PBS at a concentration of 10 g/ml. 1 g (100 l) of DT was injected intraperitoneally (i.p.) into Cx3cr1-Cre/+; Rosa-iDTR/+ mice (n = 6) every other day for 7 days.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… To induce AIA, mice were firstly immunized by subcutaneous injection of 100 µg methylated bovine serum albumin (mBSA, Sigma) in 100 µl complete Freunds adjuvant (CFA, Sigma) that contain ed 5 mg/ml heat – killed Mycobacterium tuberculosis strain H37Ra (Difco) into two sides on the hind flanks at day 0. On day 1, each mouse was intraperitoneally injected with 250 ng Pertussis toxin (List Bio Lab). At day 7 after immunization, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tracer detection:

Cholera toxin subunit B (CTB)

For the immunocytochemical detection of CTB, on slide mounted eye muscle sections and free floating brainstem sections were treated with a goat polyclonal antibody against choleragenoid (List Biological Laboratories, Cat# 703, RRID:AB_10013220; 1:40,000) for 2 days at 4C, …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… period using a commercially available ELISA (eBioscience). The effects of each compound on TNF- production were compared to cells stimulated only with LPS (R595, List Labs). …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Hormone treatment and induction of EAE:

Female PD-L1^/ and PD-L2^/ mice were implanted subcutaneously with 2.5mg/60-day release 17-estradiol pellets …Mice received pertussis toxin (Ptx, List Biologicals, Campbell, CA) on the day of immunization (75 ng) and 2 days later (200 ng). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

For EAE induction, mice were immunized s.c. over two sites on the lateral abdomen with 100 mg MOG 3555 peptide…followed by i.p. injections of 250 ng Bordetella pertussis toxin (List Biological Laboratories, Campbell, CA) on days 0 and 2 after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

C57BL/6 mice were immunized s.c. with 200 mg myelin oligodendrocyte glycoprotein (MOG)3555 peptide…followed by iv injection with 200 ng pertussis toxin (List Biological Laboratories) on…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)