Bacterial Toxin Research Citations

Infections and EAE induction:

… Mice also received two i.p. injections with 200ng of pertussis toxin (List Biologicals) at time of immunization and 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Injection of tracer:

To retrogradely trace projection neurons, animals were first anesthetized using 5 % isoflurane in oxygen, placed in a stereotaxic apparatus …through the dura mater, to the stereotaxic position of the parabrachial nucleus and 2 L of 1.0 % solution of cholera toxin subunit B (CTb) (List, Campbell, CA, USA) was slowly injected over a period of 20 min. To minimize leakage of the tracer, a 10 min waiting period was imposed before the micropipette was retracted from its position. CTb was injected 7 days prior to sacrificing the animals.

Immunohistochemistry:

… To examine lamina I projection neurons, horizontal sections were incubated in 10 % normal donkey serum (Jackson, West Grove, PA, USA) in PBS + T for 1 h at room temperature to block unspecific staining. Subsequently, spinal cord sections were incubated, for 48 h at 4 C, with the primary antibodies: goat anti-CTb at 1:5000 dilution (List Biological),

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Induction and treatment of EAE:

… Pertussis toxin (200ng; List Biological Laboratories, Campbell, CA, USA) in PBS was administered i.v. on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… SNPAtide (List Biological Laboratories, Inc., Campbell, CA). Full length BoNT/A (Metabiologics, Inc., Madison, WI). …

• Product #500 – SNAP-25 Recombinant Protein Substrate for C. botulinum Types A and E Neurotoxin

… Stimulation of the NALP1, NLRC4, or AIM2 inflammasome was achieved by treatment for 6 h with 1 g/ml protective antigen (List Biological Laboratories, Campbell, CA, USA) and 1 g/ml anthrax lethal factor (List Biological Laboratories), …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Identification of brain injection sites and CTblabelled terminals:

Brain injection sites (in the forelimb motor cortex and MLF) and the axon terminals labelled by anterograde transport of CTb were revealed using the chromogen 3, 3-diaminobenzidine (DAB; Sigma-Aldrich, UK). Brain sections and cervical spinal cord sections were incubated in goat anti-CTb (List Quadratech, USA: 1:50,000) for 48 h followed by 3 x 10 min rinses in phosphate buffered saline (PBS). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Reagents:

LPS was obtained from List Biological Laboratories (San Jose, CA, USA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Initiation of antigen-induced arthritis (AIA):

Mice were immunized on days 21 and 14 by intradermal injection of 100 µg of methylated bovine serum albumin (mBSA; Sigma-Aldrich) in 50 µl of phosphate buffered saline (PBS) emulsified in 50 µl of CFA (Sigma-Aldrich) containing 4 mg/ml of heat-killed M tuberculosis strain H37Ra (Difco). Along with each immunization, 200 ng of Bordetella pertussis toxin (List Biological Laboratories) was administered intraperitoneally. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

EAE:
Nine-week old C57BL/6J mice were randomly selected to be immunized with 100 µg MOG3555 peptide emulsified (1:1) in complete Freund’s adjuvant. Pertussis toxin (300 ng, List Laboratories, Campbell, CA, USA) was injected intraperitoneal on the day of MOG3555 immunization and two days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… An intraperitoneal injection of 300 ng pertussis toxin (List Biological laboratories, Cedarlane, Canada) was administered on the day of induction and 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)