Bacterial Toxin Research Citations

2.1. Materials:

… Cholera toxin (CT) was from List Biological Laboratories, Inc. (Campbell, CA, USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… In the experiments with LPS, animals (n = 36) received intraperitoneal (ip) saline-based injections of ultra-pure LPS (lot # 4231A1; List Biological Laboratories, Campbell, CA, USA) in the dose of 1 mg kg 1 ….

• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5

Treatment conditions:

… To determine the effect of MPLA exposure on LPS-mediated cytokine activation, HUVECs were exposed to 10 g/ml of MPLA and co-incubated in fresh media containing 0% or 2% FBS with Ultra-Pure LPS from E. coli 0111:B4 (100 ng/ml; List Biological Laboratories, Inc. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Reagents:

Ultrapurified Escherichia coli (E. coli) 0111:B4 LPS was purchased from List Biologicals Laboratory, Inc. (Campbell, CA). …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

EAE:

…Pertussis toxin (300 ng in PBS; List Biological, USA) was injected intraperitoneally at the time of induction and a second dose was administered three days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animal Models (Cell depletion):

The CD11b⁺ cells were depleted in CD11b-diphtheria toxin receptor (DTR) mice (congenic in the C57BL/6 background) by intraperitoneal injection with diphtheria toxin (DT; 7.5 ng/g body weight; List Biological Laboratories) or phosphate-buffered saline (PBS) on days 7 and 9, with sacrifice on day 10. We gave DT twice as continued DT treatment is toxic and can lead to saturation of the DTR. Effects of CD11b⁺ cell depletion were assessed on day 8 and day 10 (1 day after treatment) as CD11b⁺ cells repopulate the liver 48 hours after DT depletion.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

T cell activation for phenotyping experiments:

… As a positive control, PBMCs also stimulated with staphylococcal enterotoxin B (List Biologicals) at 5 µg/ml.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Materials:

The HEK293S GnTI cell line was obtained from ATCC. LPS derived from Salmonella minnesota R595 (Re) was purchased from List Biological Laboratories, Inc. (Campbell, CA) and used for the factor C or factor B activation assays and as free LPS to evaluate binding to LPS-immobilized Sepharose. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Macrophage depletion in diabetic CD11b-DTR mice restores PEDF protein expression to normal levels:

Male 6-wk-old B6.FVB-Tg(ITGAM-DTR/EGFP)34Lan/J (CD11b-DTR) mice (stock no. 006000, Jackson Laboratory, Bar Harbor, MN) were given multiple intraperitoneal injections of vehicle or STZ. A diphtheria toxin (DT) (List Biological Laboratories) was injected intraperitoneally at the dose of 25 ng/g body wt weekly for 6 wk after STZ injection [25]. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae