Bacterial Toxin Research Citations

Proteins and peptides:

Immobilized PA83 (LIST Biological Laboratories, Inc.) was used in the ELISA to establish titers of PA-reactive Ab in the clinical samples. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Antigen challenge to skin:

… For OVA + CT immunization, mice were immunized epicutaneously as previously described8. Briefly, we used scotch tape (3M) to gently remove the cornified layer of ear skin, and then treated the skin with acetone and cholera toxin adjuvant (List Biological Labs) before administrating chicken ovalbumin + cholera toxin.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

For mCherry expressing Salmonella infection, mice were infected intranasally with 10 9 CFU per mouse. Where specified, 1µg pertussis toxin (List Biological Laboratories, Campbell, CA) per mouse was supplemented with Salmonella.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials:

LPS from E. coli O55:B5 was purchased from List Biologicals (Campbell, CA, USA). 

Endotoxemia:

Mice were injected via tail vein with 40 g of LPS (2 mg/kg) or 4 mg of purified mouse hemoglobin or both in a volume of 200 l,…

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

Depletion of pDCs:

Recipient animals were pDC depleted with DT (100 ng/mouse, intraperitoneally [IP], 3 times per week; List Biologicals, Campbell, CA). OVA-speci fi c Treg stain with MR9-4 ant ibody (eBiosc ience) and transferred OT-II cells are identi fi ed as CTV 1.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Retrograde labeling of spinal motoneurons and dorsal root ganglion neurons:

To label spinal motoneurons and dorsal root ganglion neurons, the tracer was then placed at the cut end of sciatic nerve 1cm distal to the anastomosis in the polyethylene capsule containing a solution of 1% cholera toxin subunit B low salt (CTB; List Biological Laboratories, Campbell, CA, USA) and left in place for 30 minutes. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Macrophage isolation and cell culture:

Collected bone marrow was grown on nontissue culturetreated dishes for 6 days, then plated on tissue culture dishes,…and/or LPS (10 ng/ml; Salmonella minnesota; List Biologicals) such that BMMs were harvested on day 7.

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Experimental Autoimmune Encephalomyelitis:

… On days 0 and 2, mice received 200 µg of pertussis toxin (List Biological) intraperitoneally. Development of disease was monitored daily according to the following criteria: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell treatment and cytokine measurements:

…Treatments included each fraction or starting material alone or in combination with 10 or 100 ng/mL of LPS from Salmonella minnesota R595 (List Biological Laboratories, USA) for 1618 hr. Supernatants were collected, centrifuged at 16,000 x g for 5 min. and stored at -80C until analysis…

• Product #304 – LPS from Salmonella minnesota R595 (Re)

… Briefly, 3.75 mg (1 mg protein) of Kraft All Natural peanut butter was gavaged with 5 µg or 2.5 µg of cholera toxin (CT) (List Biological Laboratories, Campbell, CA, USA) once a week for 4 weeks or for 10 consecutive days, respectively. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae