Citations

EAE:

… To facilitate the efficiency of EAE induction, 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA) were given intraperitoneally to each mouse on the day of immunization with MOG 3555 peptide and 48 h later (total 2 doses). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

Recombinant human myelin oligodendrocyte glycoprotein (rhMOG) 1125 (rhMOG1125) was generated as previously described (5). EAE was induced by s.c. immunization at four sites on the back… On days 0 and 2, mice were injected i.p. with 300 ng pertussis toxin (List Biological Laboratories).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

The concept of a soluble SNAP-25 FRET substrate was further expanded with the design of a 13-mer peptide containing a terminal FITC fluorophore and DABCYL quencher; this product, SNAPtide, was commercialized by List Biological Laboratories, Inc. …

• Product #521 – SNAPtide Peptide Substrate (FITC/DABCYL) for C. botulinum Type A Neurotoxin

… After final behavioral testing, animals were anesthetized as described above and nerves were traced using a 3 µL injection of Cholera Toxin B subunit (CTB; 1% CTB in sterile diH 2 O; List Biological Laboratories, Campbell, USA) 10 mm distal to the crush site (63). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Reagents and Antibodies:

LPS from Escherichia coli O111:B4 was purchased from List Biological Laboratories Inc. (Campbell, CA). …

Analysis of in Vitro CSR:

Splenic B cells were stimulated with LPS (20 g/ml) or anti-CD40 antibodies and the additional reagents indicated below. …

RNA Sequence:

Total RNA were purified from LPS-stimulated (20 g/ml) splenic B cells on day 3 by RNeasy…

ChIP Assay:

Splenic B cells were activated with LPS plus IL-4 for 3 days. …

• Product #201 – LPS from Escherichia coli O111:B4

Inhibition of Cell Signaling Pathways:

… All inhibitors were obtained from Calbiochem, except for PTX (List Biological Laboratories). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

LPS was obtained from List Biological Laboratories (San Jose, CA, USA), and it was dissolved in endotoxin-free water. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Sensitization and treatment protocol:

…Sensitization began when mice were 6 to 12 weeks old. Sensitized mice received, by gavage, a mixture of PT-gliadin (500 µg) and cholera toxin (CT) (25 µg) (List Biological Laboratories Inc.) once a week for 3 weeks. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… For the experiment, the TA muscle on the immobilized side was injected with either 1 l saline (N = 5), or 1 ng tetanus toxin dissolved in 1 l saline (N = 7; List Biological Laboratories) at the time of joint fixation. …

• Product #190 – Tetanus Toxin from Clostridium tetani

Peptides and proteins:

… Recombinant protective antigen (rPA) was obtained from List Biologicals (Campbell, CA ) ….

 Vaccines and immunization:

… For anthrax and influenza protein vaccines used in Chapter 3, lipids were prepared with S100 lecithin (Lipoid GmBH, Bermany).  These vaccines were delivered via intramuscular injections of 25 L on each the right and left leg.  Each 50 L dose of anthrax vaccine contained 1 g rPA and adjuvants as indicated in figure legends.  …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis