Bacterial Toxin Research Citations

… Isolated macrophage cultures (n = 4) were treated with …ultrapure E. coli LPS (O111:B4; List Biological Lab), …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Quantum Dots:

As a positive agonist of pro-inflammatory responses, cells were treated with 10 ng/ml of E. coli LPS (List Biologicals, Campbell, CA) for 24 h for MTEC and 4 h for macrophages, in volumes of medium equivalent to those used for Qdots.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Cells, mice and reagents:

… LPS (Escherichia coli 0111:B4) from List Biological Laboratories; …

Results

Dex enhances LPS-induced expression of Lcn2 and PTX3 in macrophages:

Consistent with previous observations (30, 31), Dex suppressed expression of the NF-B-regulated cytokine genes Il6 (encoding IL-6) and Il12b (encoding IL-12 p40) in LPS-stimulated BMMs at both mRNA (Fig. 1A) and protein levels (Fig. 1B). Although LPS elicits expression of Lcn2 and Ptx3 via NF-B activation in macrophages (11, 13, 18, 32, 33), little is known whether Dex affects LPS-induced expression of Lcn2 and Ptx3. …

• Product #201 – LPS from Escherichia coli O111:B4

B cell enrichment and proliferation analysis:

B cells were enriched by negative selection using biotin-conjugated anti-CD43 (S7) antibody B cells (2 10⁵/well) were plated in 96-well flat-bottom plates and stimulated with LPS (10 g/ml) (List Biological Laboratories, Campbell, CA),

• Product #201 – LPS from Escherichia coli O111:B4

Reagents:

…Escherichia coli 0111:B4 LPS

PMN and macrophage harvests and culture:

Macrophages were stimulated with media,…LPS (List Biologicals, Campbell, CA; 10ngml¹),…for 24h and cell supernatants harvested for cytokine analysis.

• Product #201 – LPS from Escherichia coli O111:B4

Adjuvant activity of LP1569:

Mice were immunized intraperitoneally twice (0 and 4 or 5 weeks)…and pertactin, PT, and recombinant PT were obtained from List Biological Laboratories (Campbell, CA).

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

• Product #184 – Pertussis Toxin Mutant
• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

MOG35-55 peptide and SRBC immunizations:

Mice were immunized with 100-150 µg of MOG35-55 peptide (Genemed Synthesis)subcutaneously (s.c.) in an emulsion of complete Freuds Adjuvant (CFA) containing 1mg/mL of heat killed H37RA strain of Mycobacterium tuberculosis (Sigma-Aldrich) in the hind leg region. Pertussis toxin (List Biological Laboratories, Inc) in PBS was injected intraperitoneally (i.p.) at a dose of 100-250 µg on the day of immunization and again 2 days after. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

To induce EAE, J18^/, CD1d^/, IL-4^/ and WT C57BL6 mice were injected subcutaneously with 200 µg myelin oligodendrocyte glycoprotein (MOG)3555 (amino acid sequence, MEVGWYRSPFSRVVHLYRNGK; Anaspec, Fremont, CA, USA) emulsified in Freunds adjuvant (Difco, Detroit, MI, USA) supplemented with attenuated Mycobacterium tuberculosis H37RA (Difco) to 4mg ml¹. On days 0 and 2 after immunization, the mice were injected intraperitoneally (i.p.) with 200ng pertussis toxin (List Biological Laboratories; Campbell, CA, USA) in 100 l PBS. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… 200 ng pertussis toxin (List Biological Laboratories, …) in 500 µl sterile PBS (PAA, Pasching, Austria) was administered on days 0 and 2 post immunization. Clinical signs were evaluated …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.6. Induction of experimental autoimmune encephalomyelitis (EAE) and FTY720 treatment:

68 weeks old female C57BL/6 WT and Cbl-b^/ mice were immunized s.c. in the back with 165 µg of MOG₃₅₅₅ emulsified with CFA containing 300 µg of H37RA mycobacteria (Difco BD). 150 ng of Pertussis toxin (PTX) (List Biological Laboratories, Campbell, CA) was administered i.p. on day 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)