Bacterial Toxin Research Citations

… Pertussis toxin (PTX) was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Mice received three 100 µl (total volume of 300 µl) injections subcutaneously on their back. Mice also received intraperitoneal (ip) injections of 200 ng pertussis toxin (List Biological Laboratories, INC., Campbell, CA) on days 0 and 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

C4 dorsal column injury:

… Three days prior to sacrifice, animals were injected bilaterally into the sciatic nerve with a 1%wt/vol solution of the transganglionic tracer cholera toxin B (CTB; List Biological Laboratories, Campbell, CA) in dH₂O using a 36ga NanoFil syringe.

Sacrifice and tissue processing:

… Animals were deeply anaesthetized with ketamine cocktail and transcardially perfused with ice-cold PBS  Antibodies used for fluorescent immunohistochemistry were: goat anti-CTB [1:10,000; 3-day incubation] (List Biological Laboratories, Cat# 703, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Reagents:

… LPS (Escherichia coli-derived, serotype O111:B4; List Biological Laboratories, Campbell, CA);…

Generation of ₂GPI-specific T Cell Hybridoma C3hB-1.5:

The C3hB-1.5 T cell hybridoma was generated from a C57BL/6 mouse that had received four biweekly intravenous immunizations of human ₂GPI (20 g) on Day 1 and LPS (10 g) on Day 2 as described previously…

• Product #201 – LPS from Escherichia coli O111:B4

Histology and immunohistochemistry:

For the CtB experiment, CtB-injected animals were perfused 7 d after CtB injections into the LH, and an antibody directed against CtB (1:100; List Biological Laboratories) was used to localize AcbSh neurons that project into the LH.

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Reagents:

… Lipopolysaccharide (LPS) was obtained from List Biological Laboratories (Campbell, CA). TNF-, IL-1, and IFN- were used separately, as a mixture (referred to as CytoCombo), or as a mixture of CytoCombo and LPS (CytoCombo + LPS)

Fractionation of endothelial cell-derived extracellular vesicles (E-EVs):

Ten-centimeter dishes coated with type I collagen (BD Biosciences, San Jose, CA) of confluent b.End5 cells were rinsed with PBS, and stimulated with medium containing CytoCombo + LPS, CytoCombo, or LPS alone to induce inflammation.

Scanning electron microscopy:

Endothelial b.End5 cells were seeded on cover slips and cultured until confluent. Confluent cells were then exposed to inflammatory stimuli (CytoCombo + LPS) for 10 seconds or 10 minutes. After stimulation, the cells were fixed with 2% formaldehyde…

Western blotting:

For E-EV marker analyses, E-EVs that were fractionated from the culture media of b.End5 cells after CytoCombo + LPS stimulation were used as sample.

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

DC Depletion with Diphtheria Toxin:

Mgl2-DTR and huLangerin-DTR mice were i.p. injected with 1 mg of diphtheria toxin (List Biological Laboratories) 2 days before infection,…

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Reagents:

…and LPS was from List Biological Laboratories (Campbell, CA).

LPS-induced apoptosis of DCs:

To induce apoptosis of DCs in vivo, RAG22/2 and RAG22/24-1BB2/2 mice were injected i.v. with 10 mg LPS and subsequently administered s.c. with 5 3 105 MC38 tumor cells. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

…On days 0, 3, and 7 each mouse was injected ip with 0.2 ng of purified Bordetella pertussis toxin (List Biologicals, Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… After the caudal NA was located by antidromic stimulation of the vagus (1015 mV, 0.2 ms), cholera toxin B subunit (CTB; List Biological Laboratories, Campbell, CA) was microinjected (1%, 20 nl) into the NA at 0.5 mm caudal to the obex. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt