Bacterial Toxin Research Citations

Animals and vaccinations:

For rabbit experiments employing Freunds adjuvant, Female New Zealand white (NZW) rabbits (Covance Research Products, Denver, PA) weighing approximately 2.5 kg were immunized on day 0 with a mixture of two MAPs (125 g per MAP) in CFA and boosted 4 times at two-week intervals with the MAPs in IFA. For studies using human-use adjuvants, NZW rabbits were injected with TB-LND(2) or PA83 (List Labs, Campbell, CA) according to the above schedule, …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Reagents:

LPS was obtained from List Biological Laboratories (San Jose, CA, USA). …

EEM inhibits NF-B activation induced by LPS:

… Figure 1. EEM inhibits NF-B activation induced by LPS.  … (C) RAW264.7 cells were pretreated with 50 or 100 g/mL EEM for 1 h and then further stimulated with LPS (10 ng/mL) for 30 min. Cell lysates were analysed for IB and -actin protein by immunoblots. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Injection of purified toxins:

HIOs were prepared and injected as described above. For these experiments, purified C. difficile toxin A (TcdA) or B (TcdB)…152C or 155D, respectively; List Biological Laboratories), reconstituted at a concentration of 1 g/ml, aliquoted, and stored at 80C. Due to lot-to-lot variation, each new lot was tested for toxin activity using the Vero cell cytotoxicity assay. Each HIO was injected with approximately 2 l of either 12.8 ng/l TcdA or 25.6 ng/l TcdB with 1 mM FD4. For each experiment, a new aliquot of toxin was thawed and used.

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

… 1 nM cholera toxin; List Biological Laboratories, Campbell, CA. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… type culture collection, Manassas,USA) L-glutamine/phenol red D-MEM (Dulbecco’s Modified Eagle’s Me dium) / Ham’s F-12 with (Wako Osaka, Japan) 2.5% Fetal Bovine Serum (FBS)100 ng/ml (#100B; List biological laboratories, INC. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Clinical Study of Autologous Oral Mucosal Epithelial Cell Sheets for Endoscopy:

… Next, the cells were placed in trypsin and ethylenediaminetetraacetic acid (EDTA) for 20min to form single-cell suspensions, which were then seeded onto temperature-responsive culture inserts (Fig.1b) (UpCell Insert, 23.4mm in diameter; Cell Seed, Tokyo, Japan) and cultured in keratinocyte culture medium … 1 nM cholera toxin [List Biological Laboratories, Campbell, CA, USA], …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunizations and HSV-2 challenge:

… For intranasal (in) immunization, anesthetized mice received 1 µg cholera toxin (CT, List Biologicals Laboratories) with…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Oral immunization and sample collection:

… Mice were orally administered with freeze‐dried plant tissues [suspended in PBS (22 mg/350 μL) of different volume depending on the vaccine protocols] with or without adjuvants [cholera toxin (List Biological Laboratories, Inc., Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Retrograde tract tracing and immunofluorescence:

Retrograde tract tracing was performed using a 0.21% solution of cholera toxin B subunit (CTB; List Biological Labs), injected using standard stereotaxic coordinates (Paxinos and Franklin, 2001). Large areal injections of CTB were performed by pressure injection using a 33 gauge stainless steel needle and an automated syringe driver. For such injections 0.1l CTB was infused over 1 min, followed by a 5 min rest period before the needle was retracted. …

Antibody characterization:

Primary antibodies for immunofluorescence are described in Table 1.

List Biological Labs (Campbell, CA), goat polyclonal, #703
RRID: AB_10013220

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

… Cytokine assays were performed using RAW 264.7 cells in 96-well plates. TNF- secretion was stimulated with LPS from Salmonella minnesota R595 (List Biological Laboratories) at 10 ng/mL. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)