Bacterial Toxin Research Citations

Animals and induction of EAE:

… A second injection of MOG emulsion was injected in the left flank one week later. In addition, animals received an intraperitoneal injection of 500 ng pertussis toxin (List Biological Laboratories, Campbell, CA) dissolved in 0.2 mL PBS on days 0 and 2 of the study. Normal control mice were anesthetized and received sterile PBS injections of equal volume in place of MOG and pertussis immunizations. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Generation and polarization of macrophages:

…M0 macrophages were also similarly generated from fresh monocytes isolated from PBMCs by the Percoll-based method. For M1 or M2 macrophage polarization, M0 cells were treated for 2 days with 20 ng ml 1 recombinant human IFN g (Life Technologies) and 100 ng ml 1 Escherichia coli LPS (List Biological Laboratories, Campbell, CA), or 20 ng ml 1 recombinant human interleukin 4 (Life Technologies), respectively.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Eight-week-old mice were infected with 102 PFU of WNV or diluent (mock), and BBB permeability was assessed after 4 days. In other experiments, mice were treated with LPS (List Biological Laboratories, 3 mg/kg via intraperitoneal injection)…

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

EAE Model:

Mice also received 200 ng of pertussis toxin (List Biological Laboratories) by intraperitoneal injection on days 0 and 2. Classical disease severity was assessed daily by assigning clin- ical scores according to the following ascending paralysis scale: 0, no disease; 1, tail paralysis; 2, weakness of hind limbs; 3

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Methods:

Recombinant murine prolactin was tested in virgin C57BL/6 female mice aged 8 to 10 weeks. EAE was induced at day 0 by 50 µg myelin oligodendrocyte glycoprotein (MOG), peptide 35-55, …Mice were also administered two intraperitoneal injections of pertussis toxin (List Biological Labs, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animals and EAE induction:

C57BL/6J, 2D2 and Rag1^-/- mice were obtained from the Jackson Laboratories (Bar Harbor ME). IFNAR1^-/- mice (Ifnar1) were obtained from the Mutant Mouse Regional Resource Centers (MMRRC). 2D2 mice [25] were maintained under our established breeding protocol at the CHU de QubecUniversit Laval. Six (6) to 10 week old female mice were used in all experiments. For in vivo T cell transfer, stimulated cells were injected in cold PBS (Cellgro) intravenously, 5 x10⁶ cells per Rag1^-/- recipient. Recipient animals received 200 ng of pertussis toxin (List Biological Labs) intraperitoneally on day 0 (d0) and d2 post-transfer. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Botulinum Neurotoxin Type B Light Chain (BoNTA-LC) was purchased from List Biological Laboratories (CA, USA).

• Product #620A – Botulinum Neurotoxin Type B Light Chain, Recombinant

Materials and methods:  Puried CT was purchased from List Biological Laboratories. …

CT used to perform the following (refer to paper for details): 

Cell stimulation, cAMP assay and flow cytometry.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Experiments were performed on adult male C57BL/6 mice …The mice were anesthetized with isoflurane (2 %) and 9-15 nanoliters of a 0.2% solution of cholera toxin b subunit (CTb; List Biol. Lab) were injected into the intended target following craniotomy or laminectomy as described previously (Lu et al., 2000; VanderHorst and Ulfhake, 2006). …

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

Histology- antibody characterization:

Antibodies used in this study (see Table 1) were as characterized as follows. …The staining patterns with the CTb antibody depended solely upon the site of CTb injection; this antibody stains nothing in animals not injected with CTb.

Table 1…CTb, Cholera toxin b subunit, List Biological, goat polyclonal, 703, AB_2341545, 1:10,000. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Key reagents and their sources were as follows: Escherichia coli LPS serotype O1101:B4 (List Biological Laboratories), …

• Product #201 – LPS from Escherichia coli O111:B4