Bacterial Toxin Research Citations

Chronic EAE immunization:

Preparations of 200-g MOG₃₅₅₅ peptide emulsified in CFA… were injected subcutaneously into the lower flanks of 8-week-old female C57BL/6J mice (Jackson Laboratory, Bar Harbor, MN). Two intraperitoneal (i.p.) injections of 400-ng pertussis toxin each (no. 181, List Biological Laboratories,…)

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

ANIMAL EXPERIMENTS:

…At 3 weeks post infection, EAE induction treatment was commenced: mice were immunized via sub-cutaneous injection at two sites with MOG peptide MOG_35-55…, containing 4 mg/ml Mycobacterium tuberculosis H37 Ra… An intraperitoneal injection of 200 ng pertussis toxin (List Biological Laboratories Inc., Campbell, CA, USA) was also administered on the same day, with a second subsequent dose 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction, clinical evaluation and treatment protocols of EAE:

… EAE was induced by MOG₃₅₅₅ in female mice used between 8 and 10 weeks of age. Briefly, each mouse was immunized subcutaneously with 300µg of MOG₃₅₅₅ emulsified with an equal volume of complete Freund’s adjuvant…and then injected to the caudal vein with 200ng of pertussis toxin (PTX) (dissolved in 200µl PBS, List Biological Laboratories, USA) at the time of immunization and 2 days later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Dorsal column transection with sciatic nerve conditioning lesion:

… Five days before tissue collection, the left sciatic nerve was exposed again and 1.5 l of 10 mg/ml cholera toxin subunit (CT; List Biological Laboratories), a trans-ganglionic tracer, was injected into the proximal nerve section using a pulled glass capillary tube pipette.

For immunohistochemical analyses of tracer-labeled regenerated axons, a series of every second section of the T4T9 spinal cord segments (1116 sections per mouse, 100 m apart) was processed for CT immunoreactivity, with the starting section number chosen for each mouse at random. Individual sections were washed,…and incubated in goat anti-CT (List Biological Laboratories, 1:8000) for 15 h.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)
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Antibodies, reagents and medium:

…CT was from List Biological Laboratories (Campbell, CA, USA); …

Measurement of CT– and BLG-specific antibodies in mouse serum:

…For the measurement of CT-specific antibodies, Maxisorp microtitre plates (Nunc) were coated with 100 µl/well of 1 µg/ml CT in PBS and incubated overnight at room temperature. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE:

Experiments were performed on adult L71.8TG mice [6] or their wildtype littermates bred in-house…The mice also received an intraperitoneal injection of 500 ng pertussis toxin (List Biological Labs) in 200 µl 0.1M phosphate-buffered saline immediately after the first immunization with MOG and again 48 h later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

In Vitro Selection of Toxin B-Specific MREs:

… Lyophilized toxin B (List Biological Laboratories; Campbell, CA, USA) was reconstituted in pure water and covalently immobilized to carboxylic acid-coated magnetic beads …

• Product #155 – Toxin B from Clostridium difficile

Induction of EAE:

Mice were immunized subcutaneously (s.c.) with 100 µl of emulsified incomplete Freunds adjuvant (IFA) supplemented with 500 µg of Mycobacterium tuberculosis H37Ra (DIFCO) and 100 µg of MOG_35-55, and received an intraperitoneal (i.p.) injection of 200 ng of pertussis toxin (List Biological Laboratories) at the time of immunization and 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and evaluation of EAE:

Mice were injected s.c. into both flanks with 100 µg MOG₃₅₅₅ peptide…They were also injected twice i.p. with 200 ng PT (List Biological Laboratories) administered on the day of immunization and 48 hours later. Clinical assessment

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice were sensitized intragastrically (ig) with 20 mg whey (DMV International, Veghel, The Netherlands) in 0.5 mL PBS containing 10 µg cholera toxin (CT; List Biological Laboratories, Campbell, CA, USA) as an adjuvant. Sham-sensitized mice received CT alone. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae