Bacterial Toxin Research Citations

Infection, Induction and Scoring of EAE:

Female 6- to 8-week-old BALB/c mice were used throughout this study. New-born mice 6 to 12 h postpartum were inoculated i. p. with 200 PFU of wild-type MCMV (MW97. 01 strain) or 200 µl of phosphate buffered saline (PBS) as a control. EAE was induced via subcutaneous administration of 200 L of a suspension at 2 sites above the hind flanks. … Each mouse was immediately injected intraperitoneally and 48 hours later with 300 ng of pertussis toxin (List Biological Laboratories, Campbell, USA) in 100 µL of 0.9% NaCl. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and Assessment of EAE:

… Mice also received 200 ng of pertussis toxin (List Biological Laboratories) i.p. in 0.2 ml of PBS on the same day and 2 days later. Clinical signs of EAE were assessed daily with a 06 scoring system (0, no signs; 1, tail limpness; 2, impaired righting re- flex; 3, hind limb weakness; 4, hind limb paralysis; 5, hind limb paralysis with fore limb weakness; 6, death). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Double Tracing of CST and Propriospinal Neurons:

…Retrograde tracing of LPSNs was carried out at the same time by injecting 1 µl of cholera toxin subunit B (CTB; 1%; List Biological, Campbell, CA, USA) into each side of the lumbar spinal cord …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Stereotaxic Surgery, Perfusion And Tissue Processing:

… Two monkeys received a single pressure-injection of 5 µl of unconjugated cholera toxin subunit B (CTB, List Biological Laboratories, Campbell … Sections were incubated with a primary antibody against CTB raised in goat (1: 2000; List Biologicals, INC, 703) and rabbit antibody …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Reagents:

… LPS from Salmonella Minnesota R595 (Re) was purchased from List Biological Laboratories, Inc. …

THP1 Assay:

THP1-XBlue cells were grown in RPMI 1640… LPS was diluted in medium to have a final concentration of 10 ng/ml, and 30 l/well were added for 24 h at 37C. …

U937 assay:

U937 cells were grown in RPMI 1640 (Sigma) with 10% heat-inactivated FBS and 2 mM glutamine (Sigma). U937 cells were differentiated into macrophages with 25 nM of phorbol myristate acetate (Sigma) during 72 h in order to increase their responsiveness to LPS and establish adherent cell conditions. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

In vivo protocols:

Male C57BL/6J (Jackson Laboratories Bar Harbor) and endothelial NOS deficient (eNOS/) mice (12 and 24 wk old) were anesthetized with 3% isoflurane and exposed to vehicle or 23 mg/kg E. coli endotoxin (E. coli O55:B5, List Biologicals) dissolved in sterile water via supraglottic placement and aspiration. …

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

Reagents:

10 µg/mL cholera toxin (LIST Biological),…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… The SEB challenge model consisted of i.p. injection of 5 x LD₅₀ SEB followed 4 h later by a potentiating dose of lipopolysaccharide (40 g;List Biological Laboratories, Campbell, CA). Protection afforded by c19F1 was dose-dependent with complete survival observed in mice receiving 100 g of c19F1, alone or in combination with c4D7 and cPB10 (Figure 2B). Finally, mAb c4D7 was able to fully protect mice when administered as part of the tripartite cocktail. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

EAE:

… 250 µg of Mycobacterium tuberculosis (Difco). On the same day and again 48 hours later, mice received an intraperitoneal injection of 200 ng of pertussis toxin (List Biological Laboratories). As negative controls, mice were left …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis (EAE) induction:

SJL/J mice were injected subcutaneously in the flanks with 200 ml of 1 mM PLP₁₃₉₁₅₁ peptide. The emulsion solution was reconstituted complete Freunds adjuvant (CFA), composed of Freunds incomplete adjuvant (Pierce Biotechnology, Rockford, IL) containing Mycobacterium tuberculosis H37 Ra (2 mg/ml) (Difco Laboratories, Detroit, MI), and PLP_{139 151}. Mice were intravenously injected with 0.2 mg of Bordetella pertussis toxin (List Biological Laboratories, Campbell, CA), in a 100 ml final volume, on days 0 and 2 following sensitization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)