Bacterial Toxin Research Citations

… Twelve days after the optic nerve was crushed, the operated animals were anesthetized with a 7% chloral hydrate solution, and 3 µl 1% cholera toxin B subunit solution (CTB; List Biological Laboratories, Campbell, CA) was intravitreally injected. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Under aseptic conditions, the area to be injected was exposed, and rats were injected unilaterally with 2% FB plus 0.1% CTB (List Biological, Campbell, CA; four sites at 1.5 µl/site; …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Mice were injected ip with pertussis toxin (PTX; List Biological, Campbell, CA) on days 0 and 2 (100, 300, 400 or 600 ng/mouse as indicated). Two types of control mice were included in these experiments: untreated mice and mice injected with PTX and CFA without PLP. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animal care and use:

…Diphtheria toxin (DTX, List Biologicals) was injected as described previously [3], and does not affect sensory responses or body temperature when administered to wild-type mice, as previously described [3].

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… E Coli O55 (list biological, Campell, CA) …

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

Mice and immunizations:

Mice were immunized with 1 (i.v. or i.n.) or 10µg (p.o.) CT (List Biological Laboratories, Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

IPEC-J2 cell culture conditions and bacterial adherence assays:

…To test for the effects of endogenous LT expressed by the bacteria on adherence in isogenic porcine-origin ETEC strains that had not demonstrably shown an effect of LT on colonization [14], inocula were prepared and directly applied to the IPEC-J2 cells, as described above. To test the effect of exogenous LT on adherence on these strains, LT (List Biological Laboratories) at a concentration of 1, 10, or 100 ng/ml per well was added (pre-incubation) 1 h prior to bacterial inoculation. To test the potential of GM1 ganglioside to mitigate the effects of LT on adherence, LT at a concentration of 10 ng/ml or 100 ng/ml was incubated with the cells 1 h prior to their inoculation with bacteria, and 10 ng/ml or 100 ng/ml GM1 ganglioside (Sigma) was applied to the IPEC-J2 cells at the time of bacterial inoculation. Three independent experiments were performed for each assay.

Test of effects of different carbohydrates on LT production and secretion:

To test the effects of different sugars on LT secretion, CAYE media containing glucose, sucrose or fructose at 0%, 0.25%, 0.5%, 1%, or 2% were prepared. Strains were cultured in different media as described above, with aliquots taken at 0, 2, 4, and 6 h post-inoculation (PI). These samples were centrifuged (2500g, 10 min), and supernatants taken to analyze for LT concentrations by GM1-ELISA as previously described [17]. Three independent experiments were performed for each assay. 

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Ultrapure E. coli O111:B4 LPS, List Biological Laboratories, 421, …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Cell Culture and Transfection:

BT-20, DLD-1, HEK293T, HCC1937, HeLa, MDA-MB-435, MDA-MB-231, NIH 3T3, and MCF7 cells were cultured in DMEM (Cellgro) supplemented with 10% fetal bovine serum (FBS; GIBCO). ZR-75-1 cells were grown in RPMI 1640 supplemented with 10% FBS. MCF10A cells were maintained in DMEM/Hams F12 medium with 5% equine serum (Cellgro), 10 mg/ml insulin, 500 ng/ml hydrocortisone (Sigma-Aldrich), 20 ng/ml epidermal growth factor (EGF) (R&D Systems), and 100 ng/ml cholera toxin (List Biological Laboratories).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Female A/J mice were vaccinated with rPA (1 mg; List Laboratories) formulated with Al(OH) 3 (200 m g), SMIP-7.10 (25 m g), or both.

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis