Bacterial Toxin Research Citations

Cell culture studies:

…Mouse peritoneal macrophages where isolated from C57BL/6J, C3H/HeJ and CD44 ^-/- four days after an IP injection of thioglycolate as previously described [36]. …Cells were grown in DMEM…overnight, rinsed with PBS and then adherent cells treated in 1% FBS alone as a control or stimulated with LPS (List Biological Inc., Campbell, CA, #201). Cells were treated for 4 hours except for time-course experiments. …

• Product #201 – LPS from Escherichia coli O111:B4

Induction of active EAE in mice by immunization with MOG and adjuvant:

EAE was induced by procedures previously described in female wild-type C57BL/6J mice …All mice were administered 400 ng pertussis toxin (List Biological) i.p. at 0 and 48 h after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… PT, List Biologicals Laboratories, Campbell, CA, 181, Store at 4° C. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Induction of EAE:

C57BL/6 mice were immunized by subcutaneous injection of MOG_35-55 (200µg) with Freund’s complete adjuvant containing Mycobacterium tuberculosis H37Ra (Difco, Detroit, MI, USA) on the hind flanks. Pertussis toxin (PT; 200 ng; List Biological Laboratories, Campbell, CA, USA) was i.v. on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction and Evaluation:

EAE in mice was induced as described previously [24], [26]. Briefly, 6-8 weeks-old female B6 mice were immunized subcutaneously in the flanks with 100 µg of MOG3555 (MEVGWYRSPFSRVVHLYRNGK) or PLP178191 (NTWTTCQSIAFPSK, UT Southwestern Protein Chemistry Technology Center) emulsified in complete Freunds adjuvant (CFA) supplemented with 4 mg/ml Mycobacterium tuberculosis (MTB, H37Ra, Difco). On days 0 and 2 post-immunization, 250 ng of Pertussis toxin (PTX, List Biological Laboratories) was administered intraperitoneally in 100 µl of phosphate buffered saline (PBS). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
style=”color:rgb(63, 116, 185)”>Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

…To confirm the targets of the projections an additional group of male hamsters received an injection of the retrograde tracer: cholera toxin B (CTB, 0.25% w/v, List Biological, Campbell, CA, USA) in phosphate-buffered saline (PBS: pH 7.4) using the iontophoresis parameters …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Diphtheria toxin treatment:

Diphtheria toxin (DT) was from List Biological Laboratories (Cat. No. 150), reconstituted at 1 mg/ml in PBS, and frozen at 80C. Mice received 10 ng/g DT via the i.p. route in 0.20.3 ml PBS [25].

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Plasmids and RNAi:

Additional siRNAs were tested for LGN and CYK4, with a similar impact on anaphase NuMA/p150 Glued localization. Cells were incu- bated with Pertussis toxin (List Biologicals, Lot#181214A1) at 400 ng/ml for 4 h before analysis.

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Oral immunization:

Mice received sodium bicarbonate to neutralize gastric acid before oral immunization (9, 22). Thirty minutes later, the mice were immunized orally concurrently with 1 mg OVA (Sigma-Aldrich) and 10 mg cholera toxin (CT; List Biological Laboratories, Campbell, CA). This oral immunization procedure was conducted on days 0, 7, and 14.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Detection of Serum Antibodies to B. pertussis Antigens:

Nunc Maxisorp 96-well plates were coated overnight with 0.2 g/mL pertussis toxin (PT), 0.5 g/mL filamentous hemagglutinin (FHA), or 2 g/mL pertactin (PRN; List Biologicals, Campbell, CA) as described previously…

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

Product #170 is currently out of stock.  Large quantities may be obtained through Bulk Requests and Customer Orders.