Bacterial Toxin Research Citations

Neuronal tracing:

For tracer injections into the sciatic nerve to evaluate nerve regeneration, rats were anesthetized at 5 weeks post-spinal nerve injury with 4% isofluorane and the sciatic nerves at mid-thigh region ipsilateral to spinal nerve injury were exposed under aseptic conditions. A 5 l solution of 0.5% CTB (Cholera Toxin B subunit, low salt; List Labs)…were injected into the sciatic nerve at 35 sites using a Hamilton syringe.

Immunohistochemistry:

Frozen sliced sections of DRG (10 m) and spinal nerve (10 m) were serially mounted. Selected sections (at least 120 m apart from each other if from the same DRG) were incubated with primary reagents 24 h at 4 C, and with the secondary antibody for 2 hrs at room temperature. Primary antisera were: polyclonal goat anti-CTB IgG (1:5,000; List, CA),…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

4-hydroxytamoxifen (4-HT), tamoxifen and DTX injections:

DTX injections were performed as previously described… 40 ng/g of DTX (List Laboratories) was delivered by i.p. injections to mice at P21. Two treatments, separated by 72 hr, were given. Animals (mice) were sacrificed 2 to 3 weeks after the first injection.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Enzymatic activity of BoNT/A light chain. The endopeptidase activity of urea-denatured BoNT/A LC was assayed using a full length peptide SNAP-25 and the SNAPtide peptide (FITC/DABCYL; List Biologicals, Campbell, CA) as substrates. …

• Product #521 – SNAPtide Peptide Substrate (FITC/DABCYL) for C. botulinum Type A Neurotoxin

Immunohistochemistry:

Antibodies used in this study were as follows: …goat anti-CTB (List Laboratories)…

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Antibodies and Reagents:

… Pertussis toxin from LIST BIOLOGICAL LABORATORIES; …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Protein transfection:

… Bone marrowderived macrophages were primed with 10 ng/ml ultrapure LPS (List Biologicals) for 3 h to induce proIL-1b expression before protein transfection, using Profect-P1-lipidbased protein delivery reagent …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Experimental autoimmune encephalomyelitis induction:

Mice used in each experiment were 8-12-wk-old female littermates. Experimental autoimmune encephalomyelitis (EAE) was induced by s.c. injection of mice (ve per group) with 100 mg myelin oligodendrocyte protein (MOG) peptides emulsied in CFA (Chondrex). Mice were also injected i.p. with 200 mg pertussis toxin (List Biological Laboratories) on days 0, 1, and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animals and Surgery:

Male Sprague-Dawley rats (n = 9, 250430 g; Taconic Farms, Germantown, NY) were utilized for these experiments and all protocols were in accordance with the Institutional Animal Care and Use Committee at Oregon Health & Science University. Vagal afferents were identified using anterograde tracing following injection of Cholera Toxin B Subunit (CTb; 1 2 l; 1% in dH₂O; List Biological Laboratories, Campbell, CA)…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Three injections (60 nl each) of cholera toxin B subunit (CTB; 1% in distilled water; List Biological Laboratories, Campbell, CA) targeting the IML were made along the rostrocaudal extent of T10 on each side of the cord (six injections total). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Reagents:

Lyophilized recombinant PA and LF were purchased from the List Biological Laboratories, Inc. (Campbell, CA), and reconstituted in sterile water to make stock solutions with final concentrations of 1 mg/ml.

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Gene Expression Profiling:

RNA was isolated from HepG2 cells cultured under normoxic or hypoxic conditions with or without LT using TRIzol according to…

Polysome Analysis:

Polysome analysis was performed as described previously (37). In brief, HepG2 cells were pretreated with or without LT for 3 h and then cultured under hypoxic conditions for 4 h. …

Cytotoxicity Assay:

HepG2 and Hepa1c1c7 cells were cultured in 96-well plates with or without LT either 3 h prior to or at the initiation of a 24-h incubation under hypoxic conditions. …