Bacterial Toxin Research Citations

Reagents:

cholera toxin (List Biological Laboratories, Campbell, CA, USA)…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materials:

…Lipopolysaccharide (LPS)…were purchased from List Biological Laboratories, INC. (CA, USA)

In vitro phototoxicity testing:

Raw 264.7 macrophage cells were seeded in a 96-well plate at 1 10⁴ cells/well, incubated for 24 h for cell attachment. Activated macrophages were obtained by treating Raw 264.7 cells with LPS (1 g/mL) and INF- (50 ng/mL) for 24 h.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

In Vitro CD4+ T-Cell Polarization Assays:

DC subsets sorted from spleens, mLN, or cLP of mice were incubated with endotoxin-free OVA (1 mg/mL; BioVendor) and/or 0400 ng/mL LPS (List Biological Laboratories) before coculturing with OT-II nave CD4+ T cells (1:10 ratio) in DMEM…

• Product #201 – LPS from Escherichia coli O111:B4

…For peptide immunization of Balb/c mice, 100 µg of peptide was mixed with 5 µg of holotoxin CT (List Biological Laboratories, Campell, CA) in a total of 20 µl of PBS and 10 µl was administered in to each nare. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Labeling of Retinal Projections:

Six animals (three O. degus and three O. lunatus) were anesthetized as previously mentioned, and given intraocular injections (12 L, left eye) of 0.5% Cholera toxin subunit B (CTB, List Biological Laboratories) mixed with 1% DMSO.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

In vitro cell stimulations and in vivo treatments:

For PTX treatment experiments, pDC or CD11b⁺ cells were purified from 2 – 3 pooled spleens, then preincubated for 30 min with 100 ng/ml pertussis toxin from B. pertussis (List Biological Laboratories, Inc.) prior to stimulation.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents and Materials:

… Protective antigen (PA) protein of Bacillus anthracis was purchased from List Biological Laboratories, Inc. (Campbell, CA; USA). …

Rapid Peptide Specificity Analysis:

The peptide specificity analysis was performed by CE using PA (positive control), BSA, HRP, and an anti-PA mAb at 20 kV as described above. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Prostaglandin E₂ measurement:

Blood samples were transported on ice to the lab within one hour. The samples were aliquoted into sterile polypropylene tubes and were incubated at 37C on an orbital roller in the absence (control) or presence of O55:B5 LPS^b at a final concentration of 100 g/ml. After 6 h, the samples were centrifuged, 100 L of the plasma was combined with 900 L of methanol and dried by evaporation. …

Footnotes:

 b List Biological Laboratories, Campbell, California, USA. …

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

Reagents:

… Tetanus toxin was from List Biological Laboratories (Campbell, CA). …

• Product #190 – Tetanus Toxin from Clostridium tetani

In vitro protease assays:

Purified InhA1 was mixed with the substrate in protease buffer (50 mM Tris, 100 mM NaCl, 1 mM CaCl₂, 1 mM MgSO₄ [pH 7.8]) and incubated at 37C for set time points. Reaction mixtures were then placed on ice, and EDTA was added to a final concentration of 50 mM to stop the reaction. SDS buffer (2) was added, and samples were boiled for 5 min, separated by SDS-PAGE, and analyzed by Western blotting with substrate-specific antibodies. Recombinant PA (rPA), rLF, and rSodA-1 from B. anthracis were obtained from BEI Resources (ATCC, Manassas, VA). rEF was obtained from List Biological Laboratories (Campbell, CA). rNpr599 was purified as described above.

Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).