Bacterial Toxin Research Citations

… Mice were sensitized intragastrically (ig) using a blunt needle with 6 mg PE or 20 mg whey in 0.5 mL PBS with 10 µg cholera toxin (CT; List Biological Laboratories Inc, Campbell, CA, USA) as an adjuvant while sham mice received CT only (10 µg/0.5 mL PBS). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cell Culture:

… 100 ng/ml cholera toxin (List Biological Labs; Campbell, CA); …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… The cells were resuspended in cKSFM which is kera tinocyte serum-free medium (KSFM), supplemented with 50 g/ml bovine pituitary extract (BPE), 5 ng/ml human recombinant epidermal growth factor (EGF, all Gibco), and 30 ng/ml cholera toxin (LIST Biological Laboratories Inc.). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Strains and animals:

…Some mice were intranasally administered 1 µg of cholera toxin (CT; List Biological Laboratories, Compel, CA) 4 or 5 weeks prior to challenge, because these mice were controls in other experiments in which the experimental mice received immunizations using CT as an adjuvant. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materials and cell lines:

…Cholera enterotoxin was from List Biologicals (Campbell, CA)…

Cell lines were cultured in Dulbecco’s modified Eagle’s medium/Ham’s F-12 mixture…, 100 ng/ml Cholera enterotoxin, …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

…1 nm cholera toxin (List Biological Laboratories, Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Muscle-specific motoneuron identification:

We pre-labeled selected muscle afferents and motor pools in all rats by intramuscular injection of retrogradely transported fluorescent tracers together with CTB, 1 week before conducting the terminal experiments. Under aseptic conditions, the area to be injected was exposed and rats were injected unilaterally with 2% fast blue (FB, 4 sites at 1.5 l/site, Sigma, St. Louis, MO) plus 0.3% CTB (List Biologicals, Campbell, CA)

Immunohistochemisty (IHC):

Spinal cord sections containing motoneurons labeled fast blue
(soleus motor pool) and fluorogold (TA motor pool) at similar segmental levels were used for
immunohistochemistry. We used double labeled immunohistochemistry using anti-c-fos
 9 (Sc7202, Santa Cruz Inc., CA) and anti-CTB (List Biologicals., CA) or anti-c-fos and anti
choline acetyltransferase (ChAT AB144P, Millipore Inc, Temecula, CA) to map activated spinal
neurons associated with sensory afferents and activated cholinergic neurons around central canal
during locomotion in control and experimental rats, respectively.  …

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Spinal Cord:

… To identify FG, sections were incubated in rabbit anti-FG (1:10,000; Fluorochrome,
Denver, CO) overnight at room temperature (RT), rinsed in PBS and incubated for two
hours in biotinylated horse anti-rabbit secondary antibody (1:600; Vector Labs,
Burlingame, CA) followed by avidin-biotin complex (ABC Elite) (Vector Labs,
Burlingame, CA) for one hour.  The complex was then visualized with H2O2 and 3,3
diaminobenzidine (DAB; Vector Labs, Burlingame, CA) for five minutes.  Following
several PBS rinses, sections were then incubated in goat anti-CTb (1:40,000; List
Biological Labs, Campbell, CA) for one hour at RT and then at 4C overnight.  …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Mice, treatments, and procedures:

…SMD was administered by nasal instillation 30min before LPS challenge at 10, 50, 100, 200, or 300mg/kg as indicated in each figure or figure legend. …Ultrapure LPS from Salmonella minnesota from List Labs (Campbell, California) at 60 g/mouse (in PBS) was administered IV in a tail vein. …

• Product #434 – ULTRA PURE LPS from Salmonella minnesotaR595 (Re)

Experimental protocols:

For each experiment, mice received 1 mg/kg ultrapure LPS from E. coli O111:B4 (List Biological Laboratory) administered intravenously (iv) 4 h prior to intravital microscopy. This concentration is sufficiently low that all animals survived general anesthesia, and perfusion in the liver vasculature remained such that leukocyte-endothelial interactions occurred under flow conditions. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4