Bacterial Toxin Research Citations

… Diphtheria Toxin (DT) List Labs Cat#150 …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Experiment 1: diphtheria toxin treatment and EEG monitoring of Vgat ablation mice.

EEG-implanted mice injected with AAV9-CAG-frt-DTr were allowed to recover for 2 weeks after surgery (Table 1, Experiment 1), followed by a week of baseline 24/7 video-EEG monitoring. Beginning in the fourth week after virus injections, mice were treated with diphtheria toxin
or saline. Diphtheria toxin (List Biological Laboratories, #150) was prepared in sterile normal saline at 0.1 mg/ml and stored
frozen in aliquots at −80°C. Diphtheria toxin was diluted to a concentration of 5 ng/µl the day of use in normal saline. Mice were injected intraperitoneally at 20 µg/kg with diphtheria toxin once per day for 5 d. Video-EEG monitoring continued through treatment and for an additional 2–4 weeks after treatment.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

2.2 DT or saline administration

Adult mice (6–16 weeks of age) received two intramuscular (IM) injections of DT (List Biological Laboratories, Campbell, CA) at either 25 ng/g (low-dose) or 50 ng/g (high-dose), spaced 2 days apart. Some control mice were administered IM saline.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Drugs and Pharmacology

Formalin (2%, Sigma), PEG (30%, average M.W. 1500, Acros Organics), CFA (50%, Sigma), LiCl (12.5 mg/mL, Sigma), CNO (0.1 mg/mL, Tocris) and Diphtheria toxin (DT, 0.5mg/ml, List Labs) were diluted in normal saline. … DT was injected intramuscularly at a volume of 5 µL/g body weight. Mice received 3 DT injections under light isoflurane anesthesia with 48 h between injections.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Rorctd Tomato DTR mice were injected with diphtheria toxin (10 ng/g, i.p.; List Biological Laboratories, Campbell, CA, USA) using single or continuous injections, as indicated …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Three weeks after the completion of tamoxifen treatment, mice were given two intraperitoneal (i.p.) injections (three days apart) of 40 µg/kg of diphtheria toxin (DTX, List labs, 150) in saline. Three weeks after DTX treatment, mice were inoculated with IAV as described above …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Recombinant Corynebacterium diphtheriae toxin was obtained from Fisher (List Biological Laboratories, Cat. #150) …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Diphtheria toxin (DT) injection

Mice received 500 ng diphtheria toxin (DT, Cat# 150, List Biological Laboratory) in 100 µL distilled, 486 deionized water (ddH2O) through intraperitoneal injection (i.p.) (Binnewies et al., 2019) on 31, 32 487 and 34 dpi (days post-injury), …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… 2) Diphtheria Toxin: DTx (List Labs, cat# 150) was suspended in filter sterilized phosphate buffered saline (PBS) to create stock aliquots of 2 mg/mL solution, stored at -80 °C. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… For cell ablation, 126ng DT (List Biological Laboratories, cat. no. 150) in 200µl 0.9% saline was injected into mice via i.p. injection on days 0, 3 and 4. The same timeline for DT administration was followed for cell ablation, except that 300ng was given. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae