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January 30, 2024
Research Square
Duan, Y;Chen, X;Shao, H;Li, Y;Zhang, Z;Li, H;Zhao, C;Xiao, H;Wang, J;Zhang, X;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
January 30, 2024
Heliyon
Luo, H;Yang, J;Yang, X;Han, Z;Fang, Z;Huang, D;Gui, J;Ding, R;Chen, H;Cheng, L;Ma, J;Jiang, L;
Product: Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
2.3. Study design and active immunization
Action immunization was conducted as previously reported [16]. The peptides were dissolved in saline (2 mg/ml), and then fully
emulsified in an equal volume of complete Freund’s adjuvant containing 1 mg/ml heat-killed Mycobacterium tuberculosis H37Ra
[(CFA), #F5881, Sigma Aldrich, USA]. Mice were randomly assigned to experimental, negative control, or positive control groups.
Each mouse received a subcutaneous injection of emulsion (200 μl containing 200 μg of peptide) at four sites on the back, which was
repeated three times at 2, 4, and 6 weeks (Fig. 1). The GluN1359-378 peptide emulsion was injected as the positive control. For the sham
groups, an equal volume of saline emulsified in CFA was used in place of the peptide. Two hundred nanograms of pertussis toxin (1 ng/μl, #181, List Biological Laboratories, USA) was intraperitoneally administered to each mouse at the time of the last immunization and 48 h later.
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
January 27, 2024
Communications Biology
Douchant, K;He, SM;Noordhof, C;Greenlaw, J;Vancuren, S;Schroeter, K;Allen-Vercoe, E;Sjaarda, C;Vanner, SJ;Petrof, EO;Sheth, PM;Guzman, M;
Product: Toxin A from Clostridium difficile
Silver staining and western blot analysis
TcdA (1 μg) (List Biological Laboratories, Inc. California, USA) was incubated with DMC-4 (35 μl) for 1 h at 37 °C. Samples were loaded using 6X SDS loading buffer and heated at 95 °C for 10 min. All samples were loaded on to gradient SDS-PAGE gels (4-15%) after boiling. Silver staining was performed using the Pierce Silver Stain Kit as per the manufacturer’s instructions (Thermo Fisher Scientific. USA).
TcdA and TcdB (100 ng) were incubated with DMC-4 (35 μL) for 1 h at 37 °C. Samples were loaded using 6X SDS loading buffer and heated at 95 °C for 10 min. All samples were loaded into gradient SDS-PAGE gels (4-15%) after boiling. …
January 26, 2024
Available At Ssrn
Jiang, H;Cui, H;Chen, M;Li, F;Shen, X;Guo, C;Hoekel, G;Zhu, Y;Han, L;Wu, K;Holtzman, M;Liu, Q;
Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
January 26, 2024
Cells
Sur, M;Rasquinha, M;Mone, K;Massilamany, C;Lasrado, N;Gurumurthy, C;Sobel, R;Reddy, J;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
2.9. Immunization
Immunization involved Myhc-α 334–352 emulsified in complete Freund’s adjuvant (CFA) supplemented with an extract of Mycobacterium tuberculosis H37RA (Difco Laboratories, Detroit, MI, USA) to 5 mg/mL concentration [18]. The peptide emulsion was subcutaneously injected (100 µg/mouse) into the shoulder and hip regions (left and right) in split doses. In addition, animals received pertussis toxin (List Biological Laboratories, Campbell, CA, USA), intraperitoneally (100 ng/mouse) on days 0 and 2 after the first immunization.
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
January 25, 2024
Translational Psychiatry
Bernabe, CS;Caliman, IF;de Abreu, ARR;Molosh, AI;Truitt, WA;Shekhar, A;Johnson, PL;
Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
Experiment 2: Cholera toxin B microinjection
Rats received 100 nl of the retrograde tracer Cholera Toxin B subunit (CTB; 1% w/v in ACSF, List Biological Laboratories, Campbell, CA) over 5 min into the PFA three weeks prior to euthanasia as previously described [41].
• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
January 24, 2024
International Journal Of Molecular Sciences
Patel, S;Zhdanovskaya, N;Sergio, I;Cardinale, A;Rosichini, M;Varricchio, C;Pace, E;Capalbo, C;Locatelli, F;Macone, A;Velardi, E;Palermo, R;Felli, M;
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
4.1. Cell Culture and Generation of Human TEC Culture
… Cells were cultured in a humidified atmosphere of 5% CO2 in a medium composed of DMEM:Ham’s F-12 (3:1 ratio), 10% fetal calf serum (FCS) (ThermoFisher, Waltham, MA, USA), adenine (0.18 mM, Sigma-Aldrich, St. Louis, MO, USA), hydrocortisone (0.4 μg/mL, Sigma-Aldrich, St. Louis, MO, USA), insulin (5 μg/mL, Eli Lilly, Indianapolis, Indiana), cholera toxin (0.1 nM, List Labs, Campbell, CA, USA), glutamine (4 mM, ThermoFisher, Waltham, MA, USA), triiodothyronine (2 nM, Sigma), and antibiotics. Epidermal growth factor (10 ng/mL, Austral Biologicals, San Ramon, CA, USA) was added to the medium after 48 h of culture. …
• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
January 23, 2024
Biorxiv
Taranov, A;Bedolla, A;Iwasawa, E;Brown, F;Baumgartner, S;Fugate, E;Levoy, J;Crone, S;Goto, J;Luo, Y;
Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
… Recombinant Corynebacterium diphtheriae toxin was obtained from Fisher (List Biological Laboratories, Cat. #150) …
• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
January 23, 2024
Biorxiv
Chumak, T;Jullienne, A;Joakim Ek, C;Ardalan, M;Svedin, P;Quan, R;Salehi, A;Salari, S;Obenaus, A;Vexler, Z;Mallard, C;
Product: ULTRA PURE LPS from Escherichia coli O55:B5
Diet and treatment
Breeding cages were randomly assigned to one of the experimental isocaloric diets (AIN93G, BioServ): standard (Stand, BioServ #S7628), n-3 long chain (LC)-PUFA enriched (n-3, BioServ #S7630; soybean oil replaced with Menhaden Fish oil) or n-6 LC-PUFA supplemented (n-6, BioServ #S7629; based on corn oil). The detailed composition of the diets is presented in our previous publication (Chumak et al., 2022). The day of birth was defined as postnatal day (P) 0. At P9 pups received a single intraperitoneal injection of lipopolysaccharide (LPS from E. coli O55:B5, 1mg/kg, Cat# 423, List labs) or equivalent volume of saline. The dams remained on the experimental diet until offspring sacrifice (P10-P12). Efforts were taken to include equal number of pups of both sexes in each experimental group.
• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5
January 23, 2024
Biorxiv
Zhao, Q;Zhu, Y;Ren, Y;Zhao, L;Zhao, J;Yin, S;Ni, H;Zhu, R;Cheng, L;Xie, N;
Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
Diphtheria toxin (DT) injection
Mice received 500 ng diphtheria toxin (DT, Cat# 150, List Biological Laboratory) in 100 µL distilled, 486 deionized water (ddH2O) through intraperitoneal injection (i.p.) (Binnewies et al., 2019) on 31, 32 487 and 34 dpi (days post-injury), …
• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae