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4165 total record number 127 records this year

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4165 citations found

Improved skeletal muscle fatigue resistance in experimental autoimmune myositis mice following high-intensity interval training

Yamada, T;Ashida, Y;Tamai, K;Kimura, I;Yamauchi, N;Naito, A;Tokuda, N;Westerblad, H;Andersson, D;Himori, K;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Structure-guided optimization of light-activated chimeric G-protein-coupled receptors

Tichy, AM;So, WL;Gerrard, EJ;Janovjak, H;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Versican promotes T helper 17 cytotoxic inflammation and impedes oligodendrocyte precursor cell remyelination

Ghorbani, S;Jelinek, E;Jain, R;Buehner, B;Li, C;Lozinski, BM;Sarkar, S;Kaushik, DK;Dong, Y;Wight, TN;Karimi-Abdolrezaee, S;Schenk, GJ;Strijbis, EM;Geurts, J;Zhang, P;Ling, CC;Yong, VW;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Treating intrauterine adhesion using conditionally reprogrammed physiological endometrial epithelial cells

Xia, S;Wu, M;Zhou, X;Zhang, X;Ye, L;Zhang, K;Kang, Y;Liu, J;Zhang, Y;Wu, W;Dong, D;Chen, H;Li, H;

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

  • Cell isolation and propagation

    The uterine horns were collected from one 8-week-old C57BL female mice (Wuhan University Center for Animal Experiment). The Animal Ethics Committee of Wuhan University Center for Animal Experiment approved this study. Cell isolation and culture procedures were carried out according to the previous studies with minor modifications [24, 25, 29, 30]. In brief, fresh murine uterus from one female mouse was dissected and the uterine horns were collected as illustrated in Fig. 1A. Uterine horns were minced into pieces and dispersed into single cells by digestion with collagenase (StemCell, Vancouver, BC, Canada) plus trypsin. The primary mouse endometrial epithelial cells were co-cultured with irradiated mouse fibroblast 3T3 cells (J2 strain) (YongTech, Shenzhen, China) in primary epithelial culture basic medium (PECBM). PECBM contains DMEM and nutrient F-12 Ham (3:1) (v/v) (Sigma-Aldrich), supplemented with 5% FBS (GIBCO), 2 nM triiodothyronine (Sigma), 0.5% insulin–transferrin–selenium reagent (Life Technologies), 5 μg/ml transferrin (Life Technologies), 10 ng/mL epidermal growth factor (Sigma), 0.4 μg/mL hydrocortisone (Sigma), 1 nM cholera toxin (List Biological Labs), 0.5 μg/mL amphotericin B (Fungizone; Bristol-Myers Squibb), 40 μg/mL gentamicin (Gentacin; Life Technologies), and 5 to 10 mol/L Y-27632 (Enzo Life Sciences). …

    Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Lymph node fibroblastic reticular cells regulate differentiation and function of CD4 T cells via CD25

Kim, D;Kim, M;Kim, TW;Choe, YH;Noh, HS;Jeon, HM;Kim, H;Lee, Y;Hur, G;Lee, KM;Shin, K;Lee, SI;Lee, SH;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Distinct morphology of cardiac- and brown adipose tissue-projecting neurons in the stellate ganglia of mice

Barrett, MS;Hegarty, DM;Habecker, BA;Aicher, SA;

Product: Anti-Cholera Toxin B Subunit (Goat)

  • 2.6 Immunocytochemistry

    The following process was based on previously published procedures in the lab (Aicher et al., 2013; Hegarty et al., 2018). Prior to immunocytochemical processing, fixed stellate ganglia (SG) were rinsed with 0.1 M PB followed by 0.1 M Tris-buffered saline pH 7.6 (TS) and placed in 0.5% bovine serum albumin (BSA, Sigma-Aldrich) in TS for 30 min. SG were then incubated for 48 h at 4°C in primary antibody solution of goat anti-cholera toxin b (CTb) (1:25000, cat. 703, List Biological, RRID: AB_10013220) (Experiment 1), or goat anti-vesicular acetylcholine transporter (VAChT) (1:1000, cat. ABN100, EMD Millipore, RRID: AB_2630394) (Experiment 2), in 0.25% Triton X-100 (Sigma)/0.1% BSA in 0.1 M TS. SG were then rinsed and incubated for 2 h at room temperature in donkey anti-goat Alexa Fluor (AF) 647 secondary antibody (1:800, cat. 705–605–147, Jackson ImmunoResearch, RRID: AB_2340437) in 0.1% BSA in TS. SG were rinsed, whole mounted, and cover-slipped using Prolong Gold Antifade mountant (ThermoFisher). The goat anti-CTb antibody has been used extensively to detect the beta subunit of cholera toxin (Hegarty et al., 2010, 2018) and preadsorption of this antibody with CTb abolished immunolabeling in rat and rabbit spinal cord sections (Llewellyn-Smith et al., 1995). The VAchT antibody has been used extensively in recent rodent studies to identify cholinergic neurons using immunocytochemistry (Avila et al., 2020; Webber et al.,) and VAchT protein levels using Western blotting (Li et al., 2021; Yokoi et al., 2021).

    Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Polymorphisms in alpha 7 nicotinic acetylcholine receptor gene, CHRNA7, and its partially duplicated gene, CHRFAM7A, associate with increased inflammatory response in human peripheral mononuclear cells

Pattanaik, B;Hammarlund, M;Mjörnstedt, F;Ulleryd, MA;Zhong, W;Uhlén, M;Gummesson, A;Bergström, G;Johansson, ME;

Product: Unspecified List Labs LPS

  • 2.4 Peripheral blood mononuclear cells (PBMCs) functional assay; in vitro stimulation and cytokine measurement

    Out of 99 healthy individuals, blood samples from 61 individuals were available for the in vitro functional assay. Whole blood samples were prepared for PBMCs extraction facilitated by Ficoll-Paque PLUS density gradient centrifugation. Cleaned PBMCs were suspended in PBS with EDTA, and cells were counted using Bio-Rad TC20 Automated Cell Counter (Bio-Rad, CA, US). 3 × 106 cells were used for stimulation. PBMCs were challenged with or without 8.33 ng/ml of lipopolysaccharide (LPS, List Biological Laboratories Inc.) for 4 h at 37°C, centrifuged at 400 g and supernatants stored at −80°C until further analysis. All incubations were performed in triplicates and thereafter pooled. The pooled samples were analysed in singlicates using the Bio-Plex Pro Human Cytokine Grp 1 Panel 17-plex assay (Cat. #M5000031YV, Bio Rad Laboratories, Inc. Hercules, USA, including G-CSF, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12(p70), IL-13, IL-17A, MCP-1, MIP-1β, TNFα) according to the manufacturer’s protocol.

    Author did not specify which List Labs LPS product was utilized in their research.
    List Labs provides the following LPS products:

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Top-down control of hippocampal signal-to-noise by prefrontal long-range inhibition

Malik, R;Li, Y;Schamiloglu, S;Sohal, VS;

Product: Anti-Cholera Toxin B Subunit (Goat)

  • STAR★Methods
    Key resources table

    Goat anti-CTb antibody List labs 703, RRID: AB_10013220

    Inhibitory neuron (IN) marker expression in CTb tagged PFC LRG neurons

    5-7 days after CTb injection, mice were transcardially perfused with PBS followed by 4% PFA solution, and brains were post-fixed for at least one day. Coronal sections (75 μm) were obtained using a vibratome, and immuhistochemistry was performed (as described above). The following primary antibodies were used to stain for IN markers: rabbit anti-PV (Swant; diluted 1:200); rat anti-SST (Millipore, diluted 1:200); rabbit anti-VIP (Immunostar, diluted 1:200); rabbit anti-NPY (Immunostar, diluted 1:500); rabbit anti-calretinin (Immunostar, diluted 1:500); rabbit anti-nNOS (Life technologies, diluted 1:500), and goat anti-CTb (List, diluted 1:500). …

    Product #703 – Anti-Cholera Toxin B Subunit (Goat)