Bacterial Toxin Research Citations

Reagents and antibodies:  Recombinant human CCL1, mouse CCL1, and viral MCV-type II chemokine-like protein MC148…pertussis toxin (List Biological),

Chemotaxis assays:

For blocking studies, tumor cells were pretreated with 100 ng/ml PTx…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE/restimulation:

Intraperitoneal injection of pertussis toxin (400 ng per dose; List Biological Laboratories) was administered to each mouse on the day of immunization and on day 2 after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Recombinant Proteins and Other Reagents:

PA and EF were purchased from List Biological Laboratories (Campbell, CA).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

Induction of Experimental Autoimmune Encephalomyelitis (EAE):

…The day of MOG immunization was designated day 0. On day 0 and day 2 post-immunization (dpi), mice were injected intraperitoneally with 500 ng Pertussis toxin (List Biological Laboratory). … 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials and methods:

We used 16 male Sprague-Dawley rats weighing 250300 g. … To investigate the distribution of vagal motor neurons in the medulla oblongata projecting to the gastrointestinal tract, Fluorogold (Flurochrome LLC, Denver, CO) was applied to the cut end of the accessory celiac branch or the left gastric branch of the vagal trunk at the ventral wall of the subdiaphragmatic esophagus. CTb (List Biological Laboratories, Campbell, CA) was then injected into the body of stomach wall or the duodenum of the same animal. … Then two tubes were fixed with cyanoacrylate glue. Using a glass micropipette (tip diameter = 100 µm) affixed to a 10 µl Hamilton syringe, the same animal was injected with 10 µl of 1% CTb by pressure into the ventral wall of the body of stomach, including the cardia, or the ventral wall of the duodenum about 1 cm aboral to the pylorus. … we applied tracers to the left vagal branches or the ventral wall of the stomach and the duodenum, and then examined the DMV. For control experiments, we poured 10 µl of 2% Fluorogold or 1% CTb into the abdominal cavity. Three days after the application of tracers, the animals were perfused with saline followed by 500 ml of 4% paraformaldehyde-15% picric acid in 0.1 M phosphate buffer. The medulla oblongata was removed, and serial sections were made at a thickness of 70 m using a Vibratome. The sections were incubated with goat anti-CTb (#703, List Biological Laboratories, Campbell, CA; 1:5,000) for 1 day, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE induction and treatment:

… For EAE induction, mice were challenged subcutaneously in the flank with 200 g of myelin oligodendrocyte glycoprotein (MOG)₃₅₅₅ peptide … On days 0 and 2, mice were treated by intraperitoneal (i.p.) injection with 200 ng of Bordetella pertussis toxin (List Biological Laboratories, Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

hSCID Chimeras and In Vivo Plasmablast Expansion:

Antigens used in this study include Tetanus toxoid (List Biological Laboratories)…The 6- to 8-week-old female SCID/beige mice were purchased…

• Product #191 – Tetanus Toxoid from Clostridium tetani 

… Beginning at 14 days of age and again on days 21 and 35, pigs received an IP injection of 100 µg of purified Ovm (prepared in lab, Rupa et al., 2008a) adjuvanted with 10 µg of cholera toxin (List Biologicals, Campbell, CA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction and evaluation of EAE:

Mice were immunized for the induction of EAE, using either a 13 or a 23 immunization protocol…On the day of immunization each mouse received 200 ng pertussis toxin (PTX) i.v. (List Biological Laboratories, Campbell, CA).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.3. Inoculum and administration

… Native, sterile, preservative-free biologically active cholera toxin (List Biological Laboratories, Inc.) was used. Based upon spread plate cultures, the thawed inoculum contained 2.0 × 1010 CFU/dose and also contained approximately 2× that number of dead organisms. Cholera toxin (50 μg) was added to the bacterial inoculum (Lot ELH072910), and a 1 mL volume pipetted onto the absorbent pad of a Tegaderm patch (3 M Company) (Fig. 1). Preclinical cutaneous toxicology studies were performed using CT and the bacterial strain in animals by both the oral and cutaneous routes.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae