Bacterial Toxin Research Citations

Cholera Toxin B Injection:

After adequate anesthetic depth was reached, a dorsal cut in the skin was made
just above the kidney, posterior to the last rib and lateral to the midline. Rat-toothed
forceps were used to pull the fat surrounding the adrenal gland out of the incision. Care
was taken to avoid grabbing the adrenal gland with the forceps.  Once the adrenal gland
was exposed, 10 µl of 1% CTB solution (List Biological Laboratories, Inc.) was injected
into the adrenal gland using a Hamilton Syringe (Hamilton Company). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Intraperitoneal (ip) injections of 200 ng pertussis toxin in phosphate buffered saline (List Biological Laboratories, Campbell, CA) were given on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tracing study on central projections of primary afferent fibers of the ABVN:

Microinjection was carried out on 9 rats. Under anaesthesia, microsyringe contained 1% CTB (List Biological Labs, Campbell, CA, USA) solution was inserted subcutaneously into the junction of cavity of the auricular concha and postero-inferior wall of the external acoustic meatus for a depth of 23 mm along the auricular surface, and a total of 3µl 1% CTB was injected slowly. After injection, the needle was retained about one min and then withdrawn carefully.  …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Reagents:

The B fragment of the cholera toxin (CTb) and goat anti-CTb were from List Biological Laboratories (Campbell, CA). …

Intraocular Injections:

Syrian golden hamsters (Charles River, St-Constant, QC) were used for intraocular injections. …By P3, virtually all RGC axons have reached their targets [26]. To take advantage of this opportunity, 1 day after birth (P1), hamsters received a 2 l unilateral injection of a 1% solution of the beta subunit of the cholera toxin coupled to FITC (CTb-FITC), a highly sensitive anterograde tracer, in either 0.9% saline

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

Immunohistochemistry:

…The effects of intraocular injection of CB₂R agonist and inverse agonist were visualized by immunohistochemistry according to a protocol previously described [28]. Briefly, 40 m thick coronal sections of tissue were incubated in 90% methanol and 0.3% H₂O₂ in PBS for 20 min. After several washes, they were incubated in 0.1 M glycine solution for 30 min, and then blocked overnight at 4C. Sections were subsequently rinsed and immersed for 48 h at room temperature in a solution-containing goat 14000 anti-CTb diluted in blocking solution. 

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Induction and Clinical Evaluation of EAE:

EAE was induced in 8-week-old female SJL/J mice (Oriental Yeast Corp., Tokyo, Japan) by subcutaneous injection of 150 µg of PLP₁₃₉₁₅₁ (Biologica, Nagoya, Japan) peptide emulsified in complete Freunds adjuvant …In non-obese diabetic (NOD)/ShiJcl mice, EAE was induced by immunization with MOG₃₅₅₅ peptide (Biologica) emulsified in CFA at a dose of 200 µg, followed by the administration of pertussis toxin (200 ng; List Biological Laboratories Inc., Colorado, USA) on days 0 and 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Pharmacological Agents:

… pertussis toxin (PTX; G i and G o protein inhibitor) [500 mg/liter] was from List Biologicals (Campbell, CA), …

Implication of a Surface-localized GPCR:

The engagement of PLC-PKC and PKA pathways by CORT in activating Pyk2 hinted at the potential signaling role of G proteins. Consistent with previous demonstrations that the PLC-PKC pathway can be activated by PTX-insensitive G_q subunits as well as PTX-sensitive G/_i subunits (34), we observed here that pretreatment with PTX prevents CORT-triggered phosphorylation of Pyk2 and Src (Fig. 6A); these findings implicate the involvement of the – subunits of G_i (34). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cellular assays:

…To test the capacity of DCs to mature after stimulation, DCs were incubated in the presence of Ultra Pure LPS (List Biological Labs) 10 ng/ml or media overnight. Expression of costimulatory markers was assessed by ow cytometry and fold induction for each marker was calculated as MFILPS/MFIMedia. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

EAE induction:

Mice were injected i.p. with pertussis toxin (400 ng/dose; List Biological Labs) on days 0 and 2 after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… On day 0, each mouse was first given 200 ng pertussis toxin (List Biological Laboratories, Campbell, Calif., USA) in 200 µl PBS intraperitoneally (ip). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Enzyme-linked immunosorbent assay (ELISA):

A 50 L of a 5 g/mL solution of activated PA63 (List Biological Labs, Campbell, CA, USA) dissolved in 50 mM carbonate-bicarbonate buffer (pH 9.6) (Sigma-Aldrich) was used to coat each well of a 96-well transparent flat plate …

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis