Bacterial Toxin Research Citations

… Anti-Cholera Toxin B Subunit (Goat) List Biological Laboratories Cat#703; …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

4.4. EAE Induction and Clinical Score Assessment

Active EAE was induced as previously described [34]. Briefly, mice were subcutaneously injected with 200 μg of MOG35–55 peptide (Genscript, RP10245) emulsified in incomplete Freud’s adjuvant (Sigma, F5506; St. Louis, MO, USA) with mycobacterium tuberculosis H37Ra (BD Difco, BD 231141; Pittsburgh, PA, USA) and intraperitoneally injected with 200 ng of Pertussis Toxin (List Biological Laboratories, Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials

Pertussis toxin from Bordetella pertussis 180 List Biological laboratories
Pertussis toxin mutant from Bordetella pertussis 184 List Biological laboratories

3.2.3. Inhibition of Gi-coupled receptors

3.2.3.1. In-vitro inhibition

Cells were cultured as described in 3.2.2.1 with 200 ng/ml pertussis toxin (PTx) for 2 h. Control cells were incubated with a PTx inactive mutant in similar conditions.

3.2.3.2 In-vivo inhibition

To inhibit chemokine-coupled receptors, mice were i.p injected with 12,5 µg PTx. Control mice were injected with a PTx inactive mutant in similar conditions.

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #184 – Pertussis Toxin Mutant

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Pertussis toxin (PTX, cat: 181) was purchased from List Biological Laboratories (CA, USA). …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

2.2. Experimental design

… The two experimental sessions were held between two and four weeks apart to mitigate potential carry-over effects from LPS-mediated immune activation. …

The LPS infusion was prepared on-site immediately before administration, following manufacturer protocols for reconstitution and dilution under sterile conditions. In short, the product was reconstituted and initially diluted with sterile water, then diluted to the final dose in 30 mL sterile saline within 15 min of administration. For each participant, a single fresh vial of Clinical Center Reference lyophilized endotoxin (lipopolysaccharide/LPS; lot 94332B1) was used, prepared from Escherichia coli O113:H10:K by List Biological Laboratories, Inc. The infusion was administered at a slow rate of 1 mL/min over 30 min using an infusion pump (Taudorf et al., 2007). In the first session, LPS was administered at a very low dosage of 0.1 ng/kg of participant body weight, and in the second session at a low dosage of 0.4 ng/kg. …

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113

smFISH and immunostaining

… For immunostaining, sections were permeabilized in PBS + 0.3% Triton X-100, blocked for 1 h in block buffer (PBS + 0.3% Triton + 10% normal donkey serum) and incubated with primary antibodies in block buffer at 4 °C overnight. Slides were washed three times, incubated in secondary antibodies in block buffer for 1 h at room temperature and washed three times, and a coverslip was applied with ProLong Gold Antifade Reagent. Primary antibodies included: chicken anti-GFP (Aves Labs, GFP-1010, 1:1,000), rabbit anti-c-Fos (Synaptic Systems, 226 003, 1:5,000), goat anti-ChAT (Millipore, AB144P, 1:100) and goat anti-CTB (List Labs, 703, 1:1,000). Species-specific donkey secondary antibodies conjugated to Alexa Fluor 488, 568 or 647 were obtained from Life Technologies or Jackson ImmunoResearch and used at a 1:500 dilution. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Animals were given two 400 ng intraperitoneal injections of B. pertussis toxin (Cat# 181; List Biological Laboratories) on days 0 and 2 …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… 5 mg/mL heat-killed 493 Mycobacterium tuberculosis (Cat#231141, BD Bioscience), followed by a tail vein injection of 200 ng of pertussis toxin (Cat#179B, List Biological Laboratories) on day 0. Intraperitoneal injection of 200 ng of pertussis toxin was administered on day 2 …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)