Bacterial Toxin Research Citations

EAE induction

Age- and sex-matched mice were immunized with 100 ug MOG35-55 peptide (MOGp35-55) in complete Freund’s adjuvant (Sigma-Aldrich) in the rear flank s.c. The mice were further challenged with 250 ng of pertussis toxin (List Biological) on day 0 and day 2. Paralysis in mice were monitored and scored as previously described criteria.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Allergic sensitization and challenges

… For oral sensitization experiments, mice were given oral gavages on days 0 and 7 with 5 mg of grade III OVA with 0.5 mg kg−1 of cholera toxin (List Biologicals 100B, lot nos. 10165A1 and 10165A2) in a final volume of 0.25 ml of 0.2 M sodium bicarbonate buffer. Both sensitization and challenge were carried out around zeitgeber time 4 (ZT4).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

2.1. Reagents

… LPS (Escherichia coli 0111:B4) from List Biological Laboratories (Campbell, CA, USA), …

• Product #201 – LPS from Escherichia coli O111:B4

… and LPS from E. coli O55:B5 was purchased from List Biological Laboratories for in vivo studies …

• Product #203A – LPS from Escherichia coli O55:B5

… CTB (Choleragenoid, 103B) and CT (100B) were purchased from List Biological Laboratories. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… At the times of immunization and 2 d later, mice were iv injected with 400 ng of pertussis toxin (List Biological Laboratories). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

OVA/cholera toxin allergy model

OVA grade III (A5378; Sigma-Aldrich) was provided at 0.1% in drinking water, autoclaved for sterility for 3 d (Feeding, Tolerance) or not (Allergy) to initialize tolerance. All mice were then provided with regular drinking water for 1 wk. 1 mg OVA in 0.2 M sodium bicarbonate (Feeding) or 1 mg OVA + 20 µg cholera toxin (100B; List Biological) in 0.2 M sodium bicarbonate (Tolerance, Allergy) were provided once per week for 3 wk, followed by endpoint analysis 2 d after the final dose. Serum was harvested for ELISA 1 d prior to endpoint analysis. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Pertussis toxin (180) was purchased from List Biological Laboratories (Campbell, CA, US). …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

2.1. Bacterial Strains, Reagents, Growth Conditions, and Antibiotic Susceptibility

… The presence of Stx2 (List Labs, Campbell, CA, USA) was determined by Western slot-blot analysis of filtered culture supernates with an anti-Stx2 monoclonal antibody [57] that was obtained from Dr. Alison D. O’Brian (Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA). The results of these studies and other characteristics [58,59,60] are presented in Table 1.

• Product #162L – Shiga Toxin 2 from Escherichia coli