Bacterial Toxin Research Citations

Behavioral Procedures:

,,, One week prior to all behavioral sessions, rats received unilateral intracranial injections of cholera toxin subunit b (CTb; List Biological #104) into the BA.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… The tracer solution (10-40 nl) was injected by pressure using a 1µL Hamilton syringe that was inserted into each glass capillary tube (Table 1). A solution of 1% CTB (List Biological Laboratories, Inc., Campbell, CA, USA) dissolved in phosphate buffer (PB; 0.1 M, pH 7.4) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… glass micropipette with a 5µm tip was back-filled with choleratoxin b subunit (CTb; … List Biological Laboratories, Campbell … brainstem (1-in-5 series, 250µm intervals) sections were incubated with an anti-CTb antibody (1 : 12 000; List Biologicals, Campbell, CA …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Cholera toxin (CT) was purchased from List Biological Laboratories (USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Pertussis toxin, List Biological Laboratories …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Primary microglia cultures (Paper IV):

P1-P2 mouse brains were used for generation of primary microglia cultures. … Detached microglial cells were plated in 2% FBS, and treated with Induced expression of the cytoskeleton linker protein radixin in the adult brain 
0 or 100 nM lipopolysaccaride (LPS; List biological lab) after 24 hours. The cells were either collected 24 hours later and processed for RNA extraction or processed for immunocytochemistry.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Immunohistochemistry:

… The c-FOS immunostained sections were then incubated the following day in a goat polyclonal antibody against hypocretin (HCRT; 1:5000; Santa Cruz Biotechnology), a rabbit antiserum against the neuropeptide melanin concentrating hormone (MCH, 1:20,000; Phoenix Pharmaceutical), or a goat antiserum against CTb (1:40,000; List Biological Laboratories) in PBST-Az over 3 d at 4C. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Cholera toxin (CT), azide-free, was purchased from List Biological Laboratories, Inc. (USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE induction and treatment:

EAE was induced by injecting the mice subcutaneously (into the flanks) with 100 l of an emulsion containing 200 g of MOG₃₅₅₅ peptide (MEVGWYRSPFSRVVHLYRNGK) and 250 g of M. tuberculosis extract H37 Ra (Difco) in incomplete Freund adjuvant. In addition, the mice received 200 ng of pertussis toxin (List Biological Laboratories via Cedarlane Ltd.) intraperitoneally (i.p.) at day 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

CT ELISA and Western Blot Analysis:

CT ELISA was performed as previously described (17). Purified CT (List Biological Laboratories, Inc., Campbell, CA) was used to provide a standard curve. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae