Bacterial Toxin Research Citations

Supplemental Experimental Procedures:
 
Cell culture Bone marrow-derived macrophages were generated from femur exudates of mice by culture for 7 days at 37C in 5% CO2 in DMEM (Invitrogen) supplemented with 10% FCS, 5 mM HEPES, 0.2 mg/ml L- glutamine, 0.05 mM 2-ME, 50 mg/ml gentamicin sulfate, and 10,000 U/ml penicillin and streptomycin with 30% L cell-conditioned medium. Macrophages were then washed with PBS containing 1 mM EDTA, suspended in phenol red-free antibiotic-free DMEM supplemented with 5% FCS, activated with 100 ng/ml ultrapure LPS from Salmonella Minnesota (List Biologicals), and allowed to adhere for approximately 18 hr.

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

EAE:

… Pertussis toxin (200 ng in PBS; List Biological Laboratories) was injected intraperitoneally on days 0 and 2 after immunization. EAE clinical symptoms were scored daily as follows: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Surgery:

To trace the input to the MPB, six rats received brain microinjection of 1.0 % Cholera Toxin subunit B (CTB; List Biological) within the MPB [coordinates: anteroposterior (AP) 9.4 mm, lateral (L) 1.7 mm, dorsoventral (DV) 5.8 mm, as per the rat atlas of Paxinos and Watson (Paxinos and Watson, 2005)]. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Pertussis toxin was from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Measurement of Intracellular Calcium:

Cells (HFFs, HASMC, U373MG) were infected with HCMV recombinants at an MOI of 3 for 48 hours, then loaded with Fluo4-AM (Molecular Probes) for 60 min at 37°C. Pertussis toxin (PTx) was used at a concentration of 200 ng/mL to treat infected cells 24 hours prior to loading (List Biological Laboratories). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Glycoconjugate Synthesis:

… To facilitate conjugation of the OPS antigens to cationized bovine serum albumin (cBSA; Pierce), diphtheria toxin mutant CRM197 (CRM197; List Biological Laboratories, Inc.) … the activated OPS samples were added to small amber vials. …

• Product #149A – Diphtheria Toxin CRM197, Mutant

Vesicle-associated membrane protein (VAMP)-specific tetanus neurotoxin light-chain (TeNT-LC) and botulinum neurotoxins B and D light-chain (BoNT/B-LC and BoNT/D-LC, respectively) were purchased from List Biologicals Laboratories, Inc. (Campbell, CA., USA).

Assessing levels of virus production upon antigenic stimulation:

Peripheral blood mononuclear cells (PBMCs) were cultured at 5106 cells/ml in stimulation medium [RPMI+10% human AB serum+1 g/ml anti-CD28+1 g/ml anti-CD49d+1 M zidovudine (AZT)+1 M lamivudine (3TC)+1 M nevirapine (NVP)] with either 1 g/ml cytochrome c (Austral Biologicals), 1 g/ml HIV-1-p55 (Austral Biologicals), 1 g/ml tetanus toxoid (List Biological Laboratories), …

• Product #191 – Tetanus Toxoid from Clostridium tetani

Materials: 

… PA toxin was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA). …

Cloning:

In the ACE process the aptamers were initially labeled with a 6-carboxyfluorescein and incubated with increasing concentration of PA Toxin. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Recombinant Protein ELISAs:

Ninety-six well plates were coated overnight at 4 C with 1 g/well of rLF or rPA (List Biologicals, Campbell, CA). After washing with PBS-Tween and blocking with PBS/BSA, diluted pre-immune and Day 28 (4 days after a second booster) serum from each mouse was added in duplicate and incubated for 2 h at room temperature (RT). …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis