Bacterial Toxin Research Citations

… Cholera toxin (CT) was purchased from List Biological Laboratories (USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Pertussis toxin, List Biological Laboratories …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Primary microglia cultures (Paper IV):

P1-P2 mouse brains were used for generation of primary microglia cultures. … Detached microglial cells were plated in 2% FBS, and treated with Induced expression of the cytoskeleton linker protein radixin in the adult brain 
0 or 100 nM lipopolysaccaride (LPS; List biological lab) after 24 hours. The cells were either collected 24 hours later and processed for RNA extraction or processed for immunocytochemistry.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Immunohistochemistry:

… The c-FOS immunostained sections were then incubated the following day in a goat polyclonal antibody against hypocretin (HCRT; 1:5000; Santa Cruz Biotechnology), a rabbit antiserum against the neuropeptide melanin concentrating hormone (MCH, 1:20,000; Phoenix Pharmaceutical), or a goat antiserum against CTb (1:40,000; List Biological Laboratories) in PBST-Az over 3 d at 4C. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Cholera toxin (CT), azide-free, was purchased from List Biological Laboratories, Inc. (USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE induction and treatment:

EAE was induced by injecting the mice subcutaneously (into the flanks) with 100 l of an emulsion containing 200 g of MOG₃₅₅₅ peptide (MEVGWYRSPFSRVVHLYRNGK) and 250 g of M. tuberculosis extract H37 Ra (Difco) in incomplete Freund adjuvant. In addition, the mice received 200 ng of pertussis toxin (List Biological Laboratories via Cedarlane Ltd.) intraperitoneally (i.p.) at day 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

CT ELISA and Western Blot Analysis:

CT ELISA was performed as previously described (17). Purified CT (List Biological Laboratories, Inc., Campbell, CA) was used to provide a standard curve. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Measurement of cytotoxicity of diphtheria and anthrax toxin:

… To determine the protective effect of the purified nanobodies on anthrax toxin cytotoxicity, RAW cells were pre-incubated with 20 M purified nanobodies or 10 M D9R in 48-well plates (Costar) for 30 min at 37 C. Subsequently, 200 ng/ml PA (protective antigen; List Biological Labs) and 400 ng/ml LF (lethal factor; List Biological Labs) were added and the cells were incubated for 1.5 h, with one medium change after 45 min. The cell viability was determined using the MTT assay described above.

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Immunofluorescence:

… A 25µl Hamilton syringe was used to bilaterally inject 10 µl of 5mg/ml Cholera Toxin B subunit solution (CTB, List Biological Laboratories) in the intrapleural space (Mantilla et al, 2009). Rats were allowed to recover for 5 days prior to electrophysiological experiments described above (neural apnea n=3; time control n=4). Following electrophysiological measurements, animals were transcardially perfused with 4% paraformaldehyde (PFA) in 1 phosphate-buffered saline (PBS, pH 7.4). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Liver Fibrosis Models:

Adult male mice at least 6 wk of age were used. Hepatic fibrosis was induced by two times per week i.p. CCl₄ (0.4 L/g; Sigma) diluted 1:3 in olive oil (Sigma) for 4 wk (nine injections). Animals were culled at stated time points after the final CCl₄ injection. For depletion studies, DT (10 ng/g in PBS; List Biological Laboratories) or PBS control was administered to fibrotic CD11B-DTR or WT mice i.v. through the tail vein at the stated time points. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae