Citations

… tuberculosis H37Ra (Difco, Detroit, MI). 200 ng pertussis toxin (PTX; List Biological Laboratories, Campbell, CA) was administered intraperitoneally (ip) at days 0 and 2 (Reinhold et al. 2011). Treatment of EAE. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

Experimental autoimmune encephalomyelitis was induced in female mice 6-8 weeks of age by subcutaneous injection in the hind flank with 200µg MOG₃₅₅₅ peptide emulsified in 200µl complete Freund’s adjuvant (2.5mg/ml M. tuberculosis) on days 0 and 7, as described previously.^{60, 61} On days 0 and 2 mice were administered 250ng pertussis toxin intraperitoneally (List Biological Laboratories Inc., Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials:

… Pertussis toxin from List Biologicals (Campbell, CA); …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis induction:

Female mice, at least 9 wk old, were injected s.c. with 200 mg myelin oligodendrocyte protein (MOG) 3555 (Genscript, Piscataway, NJ) in CFA (Difco, Detroit, MI), with half of the mice injected behind the neck and the other half at the base of the tail. Two hours later, mice were injected i.p. with 300 ng pertussis toxin (List Biologicals, Campbell, CA), and another injection was given 24 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunization:

All studies were approved by Case Western Reserve University School of Medicine’s IACUC and animals were maintained in a NIH/AAALAC-approved facility. Chronic EAE was induced in 8-12 week old female C57BL/6 mice by subcutaneous immunization with 200µL of 200µg MOG₃₅₅₅ with complete Freund’s adjuvant. Pertussis toxin (500ng) (List Biological Laboratories, Campbell, CA) was injected IP at 0 and 2 days. Animals were sacrificed at Day 35 by transcardial perfusion with 4% paraformaldehyde. Animals were graded according to a standard clinical index: 1=limp tail; 2=hind limb weakness; 3=one limb plegic; 4=plegia of 2 limbs; 5=moribund or dead.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Shiga toxin 1 was purchased from List Biological laboratories, Inc (CA). …

• Product #161 – Shiga Toxin 1 from Escherichia coli

Neuroanatomical tracer injections and tissue preparation:

… We injected 2 µL of cholera toxin (List Biological Laboratories, Campbell, CA, USA) into the thyroarytenoid muscle at the midportion of the left vocal fold …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Visual stimuli were generated with a hand-held laser pointer or small flashlight. Once the target structure was identified by its response to visual stimulation, a glass micropipette previously filled with native purified cholera toxin B subunit (CTb; List Biological Laboratories, Campbell, CA; 0.5% in phosphate buffer 0.1 M) was lowered into the brain based on the coordinates obtained from the physiological recordings. The tracer was then pressure injected, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

 Vaccine-like treatment of EAE with peptides:

Prior to disease induction, four groups of SJL/J female mice (5-7 weeks) received three s.c. injections of either 100 µl of PBS or 100 µl (100 nmol/injection/day) of PLP-PEG-B7AP, PLP-BPI, and PLP peptides at 11, 8, and 5 days
prior to induction of disease on day 0. … In addition, 200 ng of pertussis toxin
(List Biological Laboratories, Campbell, CA) was delivered intraperitoneally (i.p.) on days 0 and
2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

DC loading with antigen and injection in vivo:

… In some experiments, DC were simultaneously treated with pertussis toxin (List Biological Laboratories, Inc, USA) at 20 ng/ml for 23 h. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)