Bacterial Toxin Research Citations

Reagents, bacterial strains, and cells:

Ultrapurified LPS from Salmonella enterica subsp. enterica serovar Minnesota R595 (S. Minnesota) was from List Biological Laboratories, Inc. (Campbell, CA). …

Stimulation with TLR ligands and S. aureus:

The following general stimulation program was followed unless otherwise stated. RAW 264.7 cells (1 106) or BMDMs (see the supplemental material) were seeded in 12-well plates in 1 ml of complete RPMI medium. After 3 h, the cells were incubated with 1 g/ml SMAMP for 1 h and then stimulated with the following concentrations of TLR ligands: LTA (10 g/ml), LPS (100 ng/ml), poly(IC) (1.0 g/ml), …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

Materials and Methods:

… LPS no. 225 Salmonella typhimurium was from List Biological Laboratories (Campbell, CA), …

Immunouorescence microscopy:

BMM on 12-mm circular coverslips were stimulated overnight with IFN-g or LPS, then prepared for immunouorescence microscopy. Cells were xed for 60 min at 37C (xative: 2% paraformaldehyde, 4.5% sucrose, 20 mM HEPES, pH 7.4, 70 mM NaCl, 10 mM KCl, 10 mM MgCl2, 2 mM EGTA, 70 mM lysine-HCl, 10 mM sodium periodate), rinsed …

• Product #225 – LPS from Salmonella typhimurium

Generation of EAE mice:

… EAE was induced as described previously (30). Briefly, SJL/J mice were injected subcutaneously in the abdominal flank on day 0 with 200 g of PLP 139151 and 400 g Mycobacteria tuberculosis H37RA (Difco, Detroit, MI) in 200 l of an emulsion of equal volumes of water and complete Freund’s adjuvant (CFA) (Difco). Age-matched control mice were injected with water and CFA only. On days 0, 3, and 7, each mouse was injected intraperitoneally with 0.2 g of purified Bordetella pertussis toxin (List Biological Laboratories, Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Antigen specific ELISA:

Antigen-specific IgG levels and total IgG were measured by ELISA as previously described[7, 8]. Antigens included … and pertussis toxin (PT, List Biological Laboratories, Campbell, CA). … Each ELISA plate included an internal standard/positive control sample for normalization between plates and between experiments performed on different days. Serum antibody titers of >120 ELISA units (EU) were considered seropositive.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and ex vivo IL-17 production:

EAE was induced according to the previously described method (21). Briefly, C57BL/6 mice were immunized subcutaneously with 100 l of an emulsion containing 100 g/mouse of MOG 3555 in 50 l of PBS and 50 l of complete Freunds adjuvant (CFA) on day 0. CFA was prepared by mixing incomplete Freunds adjuvant (Difco, Detroit, MI) with 20mg/ml of Mycobacterium tuberculosis H37RA (Difco). One hundred nanograms per mouse of pertussis toxin (List Biological Laboratories Inc., Campbell, CA) was intravenously injected on days 0, 2 and 7. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin (PTX) was obtained from List Biological Laboratories (Campbell, CA), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction, prevention, and treatment:

Mice (1216 wk old) were immunized s.c. with 50 mg MOG3555 peptide (MEVGWYRSPFSRVVHLYRNGK; AnaSpec) and 500 mg Mycobacterium tuberculosis (Difco) emulsied in incomplete Freunds adjuvant (Difco). In addition, the animals received 200 ng pertussis toxin (List Biological Laboratories) i.p. on days 0 and 2. The severity of EAE was monitored and graded on a clinical score of 05: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mouse immunizations:

Six- to eight-week-old female SJL/J mice were immunized with 300 mg AqpZ protein or a mixture containing equal ratios of homologous AqpZ peptides (AqpZ5567 [GHISGGHFNPAVT], AqpZ82101 [VGYVIAQVVGGIVAAALLYL], AqpZ174190, and AqpZ203220 [EQLWFFWVVPIVPGIIGG]) or homologous Aqp4 peptides (Aqp489101 [GHISGGHINPAVT], Aqp4116135 [VFYIAAQCLGAIIGAGILYL], AqpZ201217, and Aqp4228245 [ENHWIYWVGPIIGAVLAG]) (University of Illinois Proteomic Facility) and emulsied in CFA, as we reported previously (33, 36). Control mice were injected with CFA only. All of the mice also received 100 ng pertussis toxin (List Biological Laboratories, Campbell, CA) i.p. 24 h postimmunization (PI). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice received an intravenous injection of 200 ng pertussis toxin (List Biological Laboratories) in 100 l PBS on the same day and at 48 h after the injection of TNF- and IFN-. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell Lines and Chemicals:

… cholera toxin from List Biological Laboratories Inc. (CA, USA); …

Intestinal Fluid Secretion and Absorption Measurements in Mice:

… Loops were injected with 100 µl of phosphate-buffered saline (PBS) or PBS containing cholera toxin (1 µg/loop) with or without DQA (100 µM).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae