Bacterial Toxin Research Citations

… Pertussis toxin List Biological Laboratories Inc 180 …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Serological B. pertussis–Specific Immunoglobulin G by ELISA

Levels of antigen-specific serum immunoglobulin (Ig) G were measured as described elsewhere [21]. Nunc 96-well plates were coated with 0.2 µg/mL pertussis toxin (PT), 0.5 µg/mL filamentous hemagglutinin adhesin, 2 µg/mL pertactin, 0.2 µg/mL fimbriae 2 and 3, or 10 µg/mL lipooligosaccharide. For adenylate cyclase toxin (ACT), Bordetella intermediate protein A (BipA), and HKBp, the plates were coated with 1 µg/mL ACT, 1 µg/mL BipA, or HKBp at an optical density of 0.9 at 600 nm. All pertussis antigens were obtained from List Biologicals. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

• Product #186 – Fimbriae 2/3 from B. pertussis

• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

• Product #188L – Adenylate Cyclase Toxin, Recombinant from B. pertussis

Immunizations

8- to 12-week-old male and female mice were immunized subcutaneously with a 100 μl emulsion of 100 μg α-syn32-46 peptide (A&A peptides; San Diego, CA) dissolved in PBS with Complete Freund Adjuvant (CFA) containing 200 μg M. tuberculosis H37Ra (BD Difco Adjuvant, Fisher Scientific, Cat #DF0638-60-7; Waltham, MA). The day of immunization was designated as day 0. Mice received a boost with the same immunization dose two weeks later. Mice received intravenous injections of 400 ng of B. pertussis toxin (List Biological Laboratories, Cat #181; Campbell, CA) 14- and 16-days post-initial immunization (day 0 and 2 post boost). …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Methods
In a monocentric, randomized, double-blind, placebo-controlled, parallel-group, phase I trial, 57 healthy, smoking subjects were treated for 28 days with either oral BI 1026706 100 mg bid or placebo. At day 21, turbo-inversion recovery magnitude magnetic resonance imaging (TIRM MRI) was performed. On the last day of treatment, pre-challenge bronchoalveolar lavage fluid (BAL) and biopsies were sampled, followed by segmental LPS challenge (40 endotoxin units/kg body weight) and saline control instillation in different lung lobes. Twenty-four hours later, TIRM MRI was performed, then BAL and biopsies were collected from the challenged segments. In BAL samples, cells were differentiated for neutrophil numbers as the primary endpoint. Other endpoints included assessment of safety, biomarkers in BAL (e.g. interleukin-8 [IL-8], albumin and total protein), B1R expression in lung biopsies and TIRM score by MRI as a measure for the extent of pulmonary oedema.

… Next, Lipopolysaccharide (Endotoxin from E. coli Type O113 provided by National Institutes of Health Clinical Center, Bethesda, Maryland, USA and produced by List Biological Laboratories Inc., Campbell, California, USA) at a dose of 40 endotoxin units (E.U.)/kg …

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113

… To identify Vi/Vc neurons projecting to the RVM and PB, retrograde tracers Fluoro-Gold (FG; Fluorochrome) and cholera toxin subunit B (CTb; List Biological Solutions) were injected into RVM and PB in two male and two female animals. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… For active EAE induction, mice immunized with myelin peptides were additionally administered 250 ng of pertussis toxin (PTx; List Biologicals, Campbell, CA) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Diphtheria toxin (List Biological, catalog number: 150) …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

2.6.2. Human peripheral blood mononuclear cells (PBMCs)

… In the selectivity assay, PBMCs were treated for 60 minutes with E6742 (final concentration 0.003–10 μM) dilutions before their stimulation with TLR agonists for 24 h. The following agonists were used: TLR2 agonist, Pam3CSK4 (InvivoGen) at 10 ng/mL; TLR3 agonist, poly(I:C) (InvivoGen) at 75 μg/mL; TLR4 agonist, LPS (List Biological Lab) at 10 ng/mL; TLR5 agonist, FLA-BS (InvivoGen) at 0.4 μg/mL; TLR7/8 agonist R848 (InvivoGen) at 3 μM; and TLR9 agonist, ODN2006–DOTAP (InvivoGen/Roche) at 0.25 μM (ODN2006) and 20 μg/mL (DOTAP). IL-6 and IFN-α levels in supernatants were measured by using a standard ELISA procedure (BD Biosciences and R&D Systems, respectively).

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://www.listlabs.com/product-information/lipopolysaccharides/

Cholera toxin

Biotin-conjugated cholera toxin B subunit (CTB) used for the CRISPR screen was purchased from Life Technologies (catalog no. C-34779). Azide-free cholera holotoxin (CT) from Vibrio cholerae used for cAMP experiments was purchased from List Biological Laboratories (Campbell, CA; catalog no. 100B). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Concurrently, mice received a 200 ng dose of pertussis toxin (PTX; List Labs, Campbell, CA, USA) by ip injection. Two days later, mice were treated with a second 200 ng …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)