Bacterial Toxin Research Citations

… Recombinant, nicked PA63 from B. anthracis was obtained from List Biological Laboratories Inc., Campbell, CA. …

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

Cells and reagents

… 1 mM intermittently vortexed and incubated in warm media [37°C] until soluble), or LPS (433, List Biologicals, suspended in media) as detailed in representative figures. …

• Product #433 – LIST™ HPT™ from Escherichia coli O113

2.14. Cell depletion in mice

Diphtheria toxin receptor (DTR)-transgenic mice were intraperitoneally administered 40 μg/kg of diphtheria toxin (DT) (List Labs, Campbell, CA, USA) to deplete plasmacytoid dendritic cells (pDCs) in BDCA2-DTR mice or CD169+ cells in CD169-DTR mice. To deplete lung macrophages, mice were intranasally (100 μl/mouse) and intravenously (200 μl/mouse) treated with clodronate liposomes (Liposoma BV, Amsterdam, Netherlands).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Immunization of mice with the H2Kb-restricted SIINFEKL peptide together with the CASAC

C57BL/6 mice were immunized intraperitoneally (i.p.) with CASAC (43) to the H2Kb-restricted SIINFEKL peptide containing the following reagents and their amount per mouse in double-distilled water: antigen peptide, SIINFEKL (100 μg; AS-60193-1 Eurogentec); stimulating antibody, anti-CD40 (clone 3/23, 25 μg; MCA1143 Bio-Rad); T helper 1 cell cytokine, IFN-γ (100 ng; Peprotech); Toll-like receptor 4 (TLR4) agonist emulsion, monophosphoryl lipid A + trehalose 6,6-dimycolate emulsion (Sigma Adjuvant System, used as per manufacturer’s instructions; Sigma-Aldrich); and TLR9 agonist, CpG 1826 (25 μg; Invivogen). Final vaccine volumes were 200 μl. Vaccines are warmed to 37°C and shaken vigorously before injection. All mice are injected i.p. with PT (400 ng; List Biological Laboratories) 24 and 72 hours after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin List Biological Cat# 180 …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

In vivo experimental human endotoxaemia model and ex vivo analyses

Eight healthy (as confirmed by medical history, physical examination and routine laboratory testing) male volunteers with ages ranging from 18 years to 35 years provided written informed consent to participate in experimental endotoxaemia experiments conducted at the research unit of the intensive care department of the Radboud University Medical Center. …

A continuous endotoxin infusion regimen was used, as is described in detail elsewhere40. In short, participants were admitted to the research unit, and an antecubital vein and radial artery were cannulated to allow administration of fluids and endotoxin, and blood sampling and haemodynamic monitoring, respectively. A 3-lead ECG was recorded continuously throughout the experiment. After iso-osmolar pre-hydration (1.5 l NaCl 0.45% and glucose 2.5% administered intravenously in the hour before the start of endotoxin infusion), volunteers were intravenously challenged with a loading dose of 1 ng kg−1 bodyweight endotoxin (Escherichia coli lipopolysaccharide (LPS) type O113, lot no. 94332B1; List Biological Laboratories), directly followed by continuous infusion of 0.5 ng kg−1 h−1 for 3 h. Participants were monitored for 8 h after the endotoxin loading dose after which they were discharged from the research unit. …

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113

… Vero cells treated with purified C. difficile toxins (A and B) and antitoxin (List Biological Labs, 152C and 155C; TechLabs, T5000) were used as controls. …

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

… Anthrax protective antigen (PA) was obtained from List Biological Laboratories List Labs), and stored at 4°C. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Depletion of AMs was induced by intranasal instillation of 15ng/mouse of diphtheria toxin (DT, List Biological Laboratories) with double administration three days apart …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… In addition, pertussis toxin (200 ng; List Biological Laboratories) was injected intravenously on days 0 and 2. The severity of EAE was monitored and graded in a blinded …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)