Citations

EAE model:

Age- (7-12 wk of age) and sex-matched mice were immunized s.c. with 100 mg myelin oligodendrocyte glycoprotein (MOG)3555 peptide (MEVGWYRSPFSRVVHLYRNGK) emulsied in CFA (Difco Laboratories) with 500 mg Mycobacterium tuberculosis on day 0. Mice also received 200 ng pertussis toxin (List Biological Laboratories) by i.p. injection on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Inhibitor treatment:

For some experiments, cells were pretreated for 1 h at 37°C with a Gai inhibitor (1 mg/ml pertussis toxin; List Biologicals, Campbell, CA), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… To test this hypothesis, we used immunofluorescence to determine if Y1R are present in PVN neurons that project to RVLM, identified by prior RVLM microinjection of the retrograde tracer cholera toxin subunit B (CTb, 0.25%; List Laboratories). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… For the injection of cholera toxin subunit B (CtB) into LHb, a glass micropipette with an outside tip diameter… was pulled on a Flaming-Brown micropipette puller (Sutter Instrument Co, CA, USA) and filled with 1% CtB (List Biological Laboratories, Campbell, CA, USA …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Iontophoretic injections of 0.5% CTb (List Biologicals, Campbell, CA, USA) dissolved in 0.01 M phosphate buffer (PB; pH 7.4) were done by applying a 0.5- to 1.0-A positive current (200 ms pulses at 2 Hz for 15 min) through a chlorinated silver wire placed in a glass pipette …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Drugs and treatments:

… For pertussis toxin (PTx) experiments, cells were pretreated with PTx (List Biological Laboratories, Inc.) at a final concentration of 200 ng/ml or vehicle (in serum-free DMEM) for 24 hours, and PTx was maintained during drug stimulation. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animals and Drug Treatments:

Diphtheria toxin (DT; List Biological Laboratories) was administered intraperitoneally at 15 ng/g, on 2 consecutive days.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

To examine the time-course of axonal renervation, a cholera toxin B subunit (CTB, 5 µg/mouse, List Biol Lab) was injected into the tongue under deep anesthesia three days before fixation. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Immunohistochemistry:

… Finally, sections were incubated in 3,3 -diaminobenzidine (DAB, Sigma) and nickel ammonium sulfate, producing a purple-black reaction product in the nucleus. Subsequently, CTb was visualized by incubating the same tissue sections in goat anti-CTb (1:20,000, List Biological Laboratories) overnight, and …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Simultaneously with rMOG as well as 48 h later, each mouse received intraperitoneally 200 µL Pertussis Toxin (PTX) (1 µg/mL in PBS) (List Biological Laboratories, Inc., CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)