Citations

Bacterial Toxin Research Citations

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Page 47 out of 501
5002 citations found

Dysregulation of gd intraepithelial lymphocytes precedes Crohn’s disease-like ileitis

Xu, W;Golovchenko, N;Fong, A;Achi, S;Bucar, E;Vidmar, K;Zhang, L;Frey, M;Prinz, I;Pizarro, T;Kollias, G;Edelblum, K;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

  • Materials and Methods

    Animals.
    Mice of both sexes were used between 4-17 weeks of age and maintained on a C57BL/6
    background under specific pathogen-free (SPF) conditions in Allentown caging with aspen shavings and
    LabDiet5010 chow (Purina, St. Louis, MO) and RO water. TNFDARE/+ mice8 were rederived by the Genome
    Editing Shared Resource, Rutgers Cancer Institute of New Jersey and subsequently crossed to TcrdGDL
    mice67. TNF+/+ littermates were used as controls. For suppression assays, wildtype C57BL/6 mice or
    TcrdEGFP68 mice were used between 8-14 weeks of age. To assess IEL proliferation, 200 µg of
    5-ethynyl2′-deoxyuridine (EdU)(Sigma-Aldrich) was administered i.p. daily for 4 days. To induce gd T cell depletion
    in 4-week-old TcrdGDL; TNFDARE/+ mice, 15 ng of diphtheria toxin (List Biological, Campbell, CA) or
    endotoxin-free PBS per gram body weight was administered i.p. 24 and 48 h prior to the experiment. …

    Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Protocol for isolating human BCAM-positive corneal progenitor cells by flow cytometry and cell sorting

Sasamoto, Y;Yeung, PC;Tran, J;Frank, MH;Frank, NY;

Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Role of follicular CD8+ T cells labeled with B cell helper immunotypes in multiple sclerosis and a murine model

Ding, J;Zhang, J;Zhao, S;Jiang, D;Lu, J;Yang, S;Wang, J;Sun, Y;Huang, Y;Hu, C;Zhang, X;Zhang, J;Liu, T;Han, C;Qiao, X;Guo, J;Zhao, C;Yang, K;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Product: Diphtheria Toxin CRM197, Mutant

  • …A significant part of CRM197 in the world is produced by cultivation of the non-toxic strain C. diphtheriae C7(β197) [11]. This method is used by List Biological Labs (USA). Advantages of this method include correct folding of the target protein and its secretion into the culture medium, which facilitates further purification of the product. …

    Product #149A – Diphtheria Toxin CRM197, Mutant

Therapeutic Effects of Upadacitinib on Experimental Autoimmune Uveitis: Insights From Single-Cell Analysis

Huang, Z;Jiang, Q;Chen, J;Liu, X;Gu, C;Tao, T;Lv, J;Li, Z;Li, Z;Su, W;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

  • Experimental Autoimmune Uveitis Model Establishment

    We prepared an emulsion by adding Mycobacterium tuberculosis toxin (Difco Laboratories, Franklin Lakes, NJ, USA) to complete Freund’s adjuvant (Difco Laboratories) to achieve a concentration of 2.5 mg/mL, which was then mixed with an equal volume of interphotoreceptor retinoid-binding protein (1–20) (IRBP1–20; 2 mg/mL; GiL Biochem, Shanghai, China) PBS solution. Subsequently, 200 µL of the emulsion was subcutaneously injected into anesthetized mice for immunization. In addition, a 0.25-µg intraperitoneal injection of pertussis toxin (List Biological Laboratories, Campbell, CA, USA) was administered on the day of immunization and the following day. …

    Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
    Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
    Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
    Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Interactions between B cells and T follicular regulatory cells enhance susceptibility to Brucella infection independent of the anti-Brucella humoral response

Dadelahi, AS;Abushahba, MFN;Ponzilacqua-Silva, B;Chambers, CA;Moley, CR;Lacey, CA;Dent, AL;Skyberg, JA;

Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

  • In vivo cell depletions

    Animals were depleted of CD4+ or CD8+ T cells as previously described [19,85]. Briefly, animals were treated with 0.5 mg of rat anti-CD4 mAb GK1.5 (Leinco) or 0.2 mg rat anti-CD8 mAb 2.43 (Leinco) in 200 μl of DPBS i.p. one day prior to challenge. Treatment was repeated once weekly for the entirety of each study. For depletion of B cells, animals were treated with 250 μg of rat anti-CD20 mAB MB20-11 (BioXcell) in 200μl of DPBS i.p. one week prior to challenge [86]. Anti-CD20 treatment was repeated on day 14 post infection. Control animals for both T and B cell depletions received equivalent dosages of rat or mouse IgG (Leinco or Southern Biotech) respectively. Upon euthanasia, depletion of splenic CD4+ T, CD8+ T, or B cell populations were confirmed to be ≥ 90% effective via flow cytometry. TReg were depleted in DEREG animals by administering 1 μg DTX (List Biological Laboratories) resuspended in 100 μL DPBS i.p. to each animal on day 14 and 15 post challenge [59]. WT animals were administered an identical dose of DTX as a control. Blood was collected from animals to confirm systemic depletion of FoxP3+CD4+ T cells seven days post treatment (S3A Fig).

    Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Methylglyoxal suppresses microglia inflammatory response through NRF2-IκBζ pathway

Wei, SL;Yang, Y;Si, WY;Zhou, Y;Li, T;Du, T;Zhang, P;Li, XL;Duan, RN;Duan, RS;Yang, CL;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Sex-dependent effects of stress on insular cortex-to-nucleus accumbens synaptic plasticity

Gauthier, M;Dugast, E;Lardeux, V;Letort, K;Belnoue, L;Balado, E;Solinas, M;Belujon, P;

Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

  • Tracer injection

    Rats received a subcutaneous injection of the nonsteroidal anti-inflammatory ketoprofen
    (2.5mg/kg, s.c.). and were anesthetized with isoflurane (5% induction, 2.5% maintenance) in
    O2 before being placed in a stereotaxic frame. Animals were placed on a heating pad for the
    entire procedure. Lidocaine (1mg/kg) was injected locally before incision of the skin. Then,
    craniotomies were performed above the brain areas of interest. A retrograde tracer cholera
    toxin subunit B
    (CTB, 0.5 μl, List Labs, Campbell, CA, USA) was injected into the right NAcC
    [anteroposterior (AP), +1.1mm for males and +1.2mm for females from bregma; lateral (L),
    2.2mm from the midline; dorsal (D), -7.4mm from the skull surface]. Following injection, the
    incision was sutured, and the rats were placed in their homecage for 2 weeks before brain
    collection.

    Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

PKC modulator bryostatin-1 therapeutically targets CNS innate immunity to attenuate neuroinflammation and promote remyelination

Gharibani, P;Abramson, E;Shanmukha, S;Smith, MD;Godfrey, WH;Lee, JJ;Hu, J;Baydyuk, M;Dorion, MF;Deng, X;Guo, Y;Hwang, S;Huang, JK;Calabresi, PA;Kornberg, MD;Kim, PM;

Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer

  • EAE

    EAE was induced in 8–12 weeks old female C57BL/6J mice as previously described (17, 70). … On day 0, mice were immunized by injecting 50 μl of the emulsion subcutaneously (SC) into each of two sites on the lateral abdomen. On days 0 and 2, the mice were injected IP with 250 ng pertussis toxin (List Biologicals, catalog no. 181) dissolved in PBS. On day 7, they were weighed and began scoring.

    Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

A systems vaccinology resource to develop and test computational models of immunity

Shinde, P;Soldevila, F;Reyna, J;Aoki, M;Rasmussen, M;Willemsen, L;Kojima, M;Ha, B;Greenbaum, JA;Overton, JA;Guzman-Orozco, H;Nili, S;Orfield, S;Gygi, JP;da Silva Antunes, R;Sette, A;Grant, B;Olsen, LR;Konstorum, A;Guan, L;Ay, F;Kleinstein, SH;Peters, B;

Product: Tetanus Toxin from Clostridium tetani