Bacterial Toxin Research Citations

2.2 Induction of EAE and treatment

EAE was induced in 8- to 12-week-old mice with a s.c. injection of 150 µg myelin oligodendrocyte glycoprotein-derived 35-55 peptide (MOG35-55, MEVGWYRSPFSRVVHLYRNGK, CPC Scientific, California, US), emulsified in Incomplete Freund’s adjuvant containing 500 µg Mycobacterium tuberculosis (BD Difco, Detroit, Michigan, US) followed by an i.p. injection with 500 ng Bordetella pertussis toxin (List Biological, Campbell, California, US) on the day of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

T-cell chemotaxis

… Human T cells were incubated with 200 nM CCR6 antagonist (CCX2553, ChemoCentryx, USA) or vehicle control (DMSO) for 20 min prior to the chemotaxis assay. Preincubation of human T cells with 50 ng/mL of pertussis toxin (PTX) (List Biological Laboratories, Campbell, CA, USA) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice received i.p. injections with 200 ng of Bordetella pertussis toxin (List Biological Laboratories) day 0 and day 2 postimmunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Diphtheria toxin, VEGF, PD-1, CCR8 antibody and CCR2i treatments

DT (List Biological Laboratories) was administered to mice (1 μg per mouse in PBS) via retro-orbital injection twice on two consecutive days. For tumor transplantation experiments, DT was injected on days 8 and 9 after tumor s.c. transplantation. Mouse polyclonal neutralizing VEGF-A antibody (R&D clone AF-493-M) or control IgG (BioXcell clone BE0130) were injected on days 9, 12 and 15 (20 μg per mouse per injection) with or without DT, or on days 8, 10, 12, 14 and 17 with or without CCR8 antibody (BioLegend clone SA214G2; 240 μg per mouse per injection). …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… female B6 mice were subcutaneously immunized with 150 µg human IRBP1-20 emulsified with CFA at the hind foot pad and at six points on the back. Before the injection, mice received 300 ng pertussis toxin (List Labs, USA) intraperitoneally. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Active EAE

Active EAE (aEAE) was induced in 8–12 weeks old female WT C57BL/6 J using the MOGaa35-55-peptide as previously described63,65. Pertussis toxin (List Biological Laboratories, Campbell, US) (300 ng in 100 μl PBS/mouse) was applied intraperitoneal (i.p.) on day 0 (immunization day) and day 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Intragastric model of sensitization

Mice were sensitized with either 3.75 mg All-Natural Smooth Peanut Butter (Kraft Heinz, Northfield, USA), 1 mg ovalbumin (OVA) (MilliporeSigma, A5378), or 1 mg 4-hydroxy-3-nitrophenyl (NP)-OVA (LGC Biosearch Technologies, N-5051-100) by oral gavage with 5 μg cholera toxin (List Biological Labs, 100B) in 0.5 ml PBS. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

2.2. General session procedures

We performed all LPS-related procedures as described previously (Jansen et al., 2022). We tested three participants simultaneously in one room at the Medium Care Unit of Radboudumc. Sixty to 90 min before LPS administration, we placed a radial artery catheter and antebrachial venous cannula to allow serial blood sampling, hemodynamic monitoring, and administration of fluids and LPS, respectively and these stayed in during the remaining of the test-day. In the 45 min prior to LPS administration, we administered hydration fluids (2.5% glucose / 0.45% sodium chloride) as a 1.5L prehydration bolus to reduce the risk of vasovagal collapse(van Eijk et al., 2004), and thereafter at a rate of 150 mL/h for the remainder of the experiment. Directly after prehydration, we administered a bodyweight-adjusted bolus dose of 1 ng/kg LPS (E. Coli-derived, Type O113, lot no. 94332B1; List Biological Laboratories) intravenously. Behavioural testing took place at three timepoints: 45 min before injection (S1), 2 h post injection (S2) and 5 h post injection (S3). Timing was based on previous studies showing that sickness symptoms and cytokines peak 1.5–2 h after LPS administration and are largely normalized 5 post-LPS administration (Kox et al., 2015, Leentjens et al., 2012). At S2, participants were able to perform behavioural tasks while cytokine levels and fatigue are still high (Draper et al., 2018). Participants filled out mood questionnaires each hour after LPS administration. Furthermore, we obtained blood samples at various timepoints prior to and following LPS administration (see below). …

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113

… For cell-type specific ablations, diphtheria toxin (30 ng/g of mouse; List Biological Laboratories, Inc.) was injected intraperitoneally at P12 for PC-X and PC-eX animals …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Microspheres were then incubated with 6.25 µg of PT, FHA, PRN (List Labs), or TT (Calbiochem®) for 2 h at RT in the dark …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

• Product #187 – Pertactin from B. pertussis (69 kDa Protein)