Bacterial Toxin Research Citations

… Bordetella pertussis toxin (PTX) was from List Biological Laboratories (Campbell, CA, USA) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and Evaluation of EAE:

Mice were immunized s.c. in the flanks with 200 µg of myelin oligodendrocyte glycoprotein (MOG)_35-55 peptide (synthesized by the Tufts University Core Facility Laboratory) in a 200µL emulsion of complete freund’s adjuvant (CFA) containing 5 mg/mL heat-killed Mycobacterium tuberculosis H37Ra extract (Sigma-Aldrich, St. Louis, MO) on day 0 and i.p. injected with 200 ng per mouse of pertussis toxin (List Biological Laboratories, Campbell, CA) on days 0 and 2. The clinical symptoms were scored daily from day 0 to 30 after immunization, as follows: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis disease models:

Mice were immunized with MOG3555 peptide emulsied in CFA (Sigma) containing 4 mg/ml Mycobacterium tuberculosis extract H37Ra (Difco) in 100 ml/mouse s.c. distributed between the two hind anks and above the sternum on day 0. On days 0 and 2, 100 mg pertussis toxin (List Biological Laboratories) was given i.v. Mice were monitored daily and clinical scores were given as follows: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Regulatory T cell transfer and Foxp3+ T cell ablation:

Spleens and lymph nodes from either WT or Foxp3DTR B6 3 B6.g7 mice were harvested, and polyclonal CD4+ T cells were puried by negative selection using the CD4+ T cell isolation kit and an LS column (Miltenyi Biotec). To deplete Foxp3-expressing cells from reconstituting polyclonal Foxp3DTR CD4+ T cell populations, DT (List Biological, Campbell, CA) was injected i.p. at a concentration of 50 mg/kg body weight on days 8, 10, and 12 after CD4+ T cell reconstitution (19). …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

DC and NK Cell Depletion:

Depletion of cDCs was induced in CD11c-DTR-Gfp mice as described.35 Briefly, 100 ng (4 ng/g) of diphtheria toxin (DT) (List Biological Laboratories Inc.) was administered intraperitoneally 1 day after the last dose of CCl₄. Three days later, the mice were sacrificed and livers were harvested (Supporting Fig. 2B). NK cell depletion using anti-asialo-GM1 antibody (Wako Chemicals) and NKp46-DTR-eGFP mice were performed as described32, 36 (Supporting Fig. 2F,G).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Materials and Method:

Experiments using nine C57BL/6J mice were performed according to lab procedures approved by University of California at San Diego Laboratory Animal Care and Use

Animals:

Injections into the medulla used purified Cholera toxin B subunit (CTb) isolated from Vibrio cholera (Product #103b, List Biological Laboratories, Inc.). … primary antibody diluted 1:10,000 (Product #703, List Biological Laboratories, Inc.) left rotating overnight. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Recording

We recorded neuronal activity with a tungsten electrode (Microprobe Inc., impedance 0.6–1.2 MOhms) attached to a glass capillary with internal tip diameters between 8 and 25 μm (Plowden and Thompson Ltd, outer diameter 0.9 mm, internal diameter 0.12 or 0.22 mm; Fig. 1A) and containing the tracer, CTb (1% low salt solution in double distilled H2O, List Biological Laboratories Inc.). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Enzyme-Linked ImmunoSorbent Assay (ELISA):

96 well microtiter plates (EIR/RIA plate, Costar, Lowell, MA) were coated with 100 l rPA (List labs, Campbell, CA) at 1 g/ml in PBS overnight (o/n) at 4C. Plates were subsequently blocked with 200 l 1% BSA (in PBS containing 0.05% Tween 20, PBS-T) for two hours at 4C. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

TLR Stimulation and Cytokine Measurement:

For ELISA, day 5 BM-derived macrophages were plated in a 96-well tissue plate (5 104 cells per well) overnight. Titrations of TLR stimuli were added for 16 h as follows: Salmonella minnesota R595 LPS (List Biological Laboratories), …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Materials and Methods:

SNAPtide substrates (Products #521 and #523), Unquenched Calibration Peptide for SNAPtide 521 (Prod #528), BoNT/A LC (Product #610A), and BoNT/A holotoxin (Prod #130A) are products of List Biological Laboratories, Inc. …

• Product #521 – SNAPtide Peptide Substrate (FITC/DABCYL) for C. botulinum Type A Neurotoxin
• Product #523 – SNAPtide Peptide Substrate flP6(DABCYL/5-IAF) for C. botulinum Type A Neurotoxin
• Product #528 – Unquenched Calibration Peptide for SNAPtide 521 Substrate for C. botulinum Type A Neurotoxin
• Product #610A – Botulinum Neurotoxin Type A Light Chain, Recombinant
• Product #130A – Botulinum Neurotoxin Type A from Clostridium botulinum

Methods – The above materials were used in the following (refer to poster for details):

• Fluorimetric Assay
• Buffer Optimization
• Linearity
• Sensitivity and LOD
• Kinetic parameter evaluations
• Inner Filter Effect Correction