Bacterial Toxin Research Citations

Materials:

… PTX was purchased from List Biological Laboratories (Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… PA63 and LF were purchased from List Biological Laboratories (Campbell, CA). …

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

ELISA:

… An in-house ELISA was used for measuring anti-diphtheria toxoid (DT). In brief, plates (Medisorp, Thermo Scientific Nunc) were coated with 50l/well DT (1g/ml in PBS) (List Biological Laboratories, Campbell, CA, USA) and blocked with 3% BSA in T-PBS. The sera samples were diluted 1:100 in 3% BSA in T-PBS. Anti-human IgG antibody conjugated with HRP and a substrate was used in the manner described above.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

In vivo depletion experiments:

To deplete CD207+ DCs or LCs, 1 g of DT (List Biological Laboratories) was injected i.p. in Lang-DTR mice at day 3 or 15, respectively. DEREG mice were depleted of CD4+Foxp3+ Tregs (8) by i.p. injection of 1 g DT at days 2 or 1 with respect to day 0 of LC transfer.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Experimental Autoimmune Encephalomyelitis Model:

EAE was elicited and scored as previously described ³³. Briefly, on day 0 mice received bilateral subcutaneous flank injection of 50 µg of MOG (MEVGWYRSPFSRVVHLYRNGK) peptide in complete Freunds adjuvant (CFA) containing 500 µg of heat-inactivated Mycobacterium tuberculosis (Difco Labs). A dose of 200 ng of Bordetella pertussis toxin (PT; LIST Biological Labs) was injected i.p. on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunohistochemical experiments:

Retrograde labeling of phrenic motor neurons. To identify phrenic motor neurons, 12 rats were used for retrograde labeling with cholera toxin B (CtB) subunit via intrapleural injection (Mantillaetal.,2009).  Isoflurane anesthesia was induced in a closed chamber and maintained via nose cone (1.5% isoflurane in 100% oxygen). A total of 25 µl of 0.5% CtB subunit (dissolved in 0.9% sterile saline; List Biological Laboratories) was injected into the right and left thoracic cavities (6 mm deep, fifth intercostal space) using a 25 µl Hamilton syringe and a custom-made needle …
• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction of EAE with MOG₃₅₅₅ peptide:

EAE was induced as described previously [21], [23], [24] by subcutaneous injection into the mouse flank on day 0 with 300 µg of MOG₃₅₅₅ peptide thoroughly emulsified in complete Freund’s adjuvant (CFA) containing 500 µg of heat-inactivated Mycobacterium tuberculosis (Difco, Detroit, MI). One week later (day 7), the mice were boosted with 300 µg of MOG₃₅₅₅ peptide subcutaneously in the other flank. 500 ng Pertussis toxin (List Biologicals, Campbell, CA) in 200 µl of PBS was injected intraperitoneally on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Immature DCs with or without nicotine (NiDCs and MoDCs, respectively) were cultured with 10 ng/ml lipopolysaccharide (LPS; Salmonella minnesota; List Biological Laboratories, INC, Campbell, CA) to induce cytokine and chemokine production. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Chemical inhibition of RhoGTPases:

Difficile toxin B (TcdB, List Biologicals, Campbell, CA) was used to pharmacologically inhibit Rho, Rac and Cdc42 …

• Product #155 – Toxin B from Clostridium difficile

ELISA:

All ELISAs were read at 450 nm using a 1420 Victor3 (PerkinElmer) plate reader, unless otherwise indicated. Total Ig in cell culture supernatant was quantied by IgM, IgG, or IgA ELISA kits (Zeptometrix), used according to manufacturers instructions. To monitor Ag-specic Ig production, 96-well Maxisorp plates (Nunc) were coated with either heat-killed Opa2 gonococci (N302), keyhole limpet hemocyanin (KLH; Sigma-Aldrich), or tetanus toxoid (TT; List Biological Labs) resuspended in pH 7.4 PBS (Wisent). …

• Product #191 – Tetanus Toxoid from Clostridium tetani