Bacterial Toxin Research Citations

… Human MDA PCa 2b cell line (AR-positive) was maintained in Kaighn’s modification of Ham’s F12 (F12K) medium supplemented with 25 ng/ml cholera toxin (List Biological Laboratories, Campbell, CA), 10 ng/ml epidermal growth factor, 100 pg/ml hydrocortisone, 1 ITS-X …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Oral CT/OVA immunization:

Mice were immunized orally by gavage on day 0 and day 14 with CT (20 µg, List Laboratories) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunohistochemistry – Primary Antibodies:

… Cholera toxin B subunit, B subunit (choleragenoid) of cholera toxin isolated from Vibrio cholerae type Inaba 569B, List Biological Laboratories (Campbell, CA, USA), goat polyclonal, Catalog # 703; …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

PTx (List Biological Laboratories) was dissolved in PBS (100 ng/l).

Experimental autoimmune encephalomyelitis (EAE):

… PTx facilitates T cell access to the CNS, presumably via TLR4-mediated induction of adhesion molecules in the endothelium of the blood-brain barrier84. In addition, PTx contributes to the break of T cell tolerance, promoting clonal expansion and cytokine production by T cells79,80. This immunization model promotes the activation of TH cells, specifically of the TH1 and TH17 lineages79,80. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Freund’s adjuvant (CFA, heat-killed H37Ra, Difco). Mice received pertussis toxin (PTx, List Biologicals, Campbell, CA) on the day of immunization (75 ng) and 2 days later (200 ng). Animals were monitored daily for clinical signs …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of experimental autoimmune myocarditis:

Previously well-established methods were used to induce EAM [14]. Briefly,100 µg commercially fMOC synthesized MyHC₆₁₄₆₂₉ [15] was emulsified 1:1 in CFA (Sigma, St. Louis, MO) supplemented with 5 mg/mL of heat-killed M. tb strain H37Ra (Difco, Detroit, MI), and installed sc in mice twice on days 0 and 7. On day 0, mice also received 500 ng of pertussis toxin ip (List Biologicals, Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and treatment of EAE:

SJL/J female mice (57 weeks-old) were immunized subcutaneously (s.c.) with 200 µg PLP in a 0.2 ml emulsion comprised of equal volumes of PBS and complete Freund’s adjuvant (CFA) containing killed mycobacterium tuberculosis strain H37RA (at a final concentration of 4 mg/ml, Difco, Detroit, MI). The PLP/CFA emulsion was administered to regions above the shoulder and the flanks (total 4 sites; 50 µl at each injection site). In addition, 200 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) was injected intraperitoneally (i.p.) on the day of immunization (day 0) and 48 h post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Degranulation assay:

Mice were immunized with 10 mg MOG 3555 peptide emulsied in CFA (Difco) supplemented with 4 mg/ml Mycobacterium tuberculosis (H37RA; Difco). Each mouse also received 200 ng pertussis toxin (List Biological Laboratories) intravenously on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

Mice were immunized with MOG peptide 3555 (300 µg/mouse) and Mycobacterium tuberculosis (500 µg/mouse) emulsified in Complete Freunds Adjuvant (CFA; Difco Laboratories, Detroit, MI) subcutaneously, in a volume of 0.1 ml/mouse over two sites: the right draining inguinal and axillary lymph nodes. One week later a booster immunization was delivered subcutaneously, over the contralateral draining lymph nodes. Pertussis toxin (500 ng/mouse) (PTX; List Biological Laboratories, Inc., Campbell, CA) was injected intraperitoneally on days 0 and 2 …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… injections of two different tracers into subdivisions of the mesopallium, … and cholera toxin B subunit (CTB, choleragenoid; List Biological Laboratories, Campbell, CA …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt