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August 30, 2010
PLoS One
Hoffmann C, Galle M, Dilling S, Kppeli R, Mller AJ, Songhet P, Beyaert R, Hardt WD
Product: Unspecified List Labs LPS
Cell culture and infection experiments:
RAW264.7 macrophage-like cells (ATCC No. TIB-71) were cultured in RPMI (10% FCS, 1% L-Glutamine) at 37C/5% CO2. For infection experiments for analysis of LDH and Il-1 release, cells were seeded in 96 well plates the day before. 20000 RAW264.7 cells were seeded per well and were pre-stimulated with 1 g/ml E. coli LPS (List Biological Laboratories, California) overnight to induce expression of pro-IL-1. …
Author did not specify which List Labs LPS product was utilized in their research. List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/
August 19, 2010
Toxins
Hunt T, Rupp D, Shimizu G, Tam K, Weidler J, Xie J.
Product: SYNTAXtide® (o-Abz/Dnp) Peptide Substrate for C. botulinum Type C Neurotoxin
Materials and Methods:
… The light-chain activity and cleavage pattern of these BoNT/A products were assessed using the novel LCA-HPLC method reported here with BoNT/A substrate (SNAPtide520; List Biological Laboratories, Inc.; Campbell, CA, USA). SNAPtide520 sequence: Abz Thr D-Arg Ile Asp Gln Ala Asn Gln Arg Ala Thr Lys Nle Lys Dnp; where Abz = o-Aminobenzoic acid (FRET pair fluorophore) and Dnp = 2,4-Dinitrophenyl (FRET pair quencher).
BoNT/A cleavage fragment (designated as SNAPtide529 by List Biological Laboratories, Inc.) was utilized to identify retention time of the fragment. …
Commercially available BoNT/C FRET substrate (SYNTAXtide560; List Biological Laboratories, Inc.; Campbell, CA, USA) was utilized to assess type-C cleavage. Because of the RP-HPLC reaction-product readout, no modification to the method was necessary in order to observe the reaction products generated using the SYNTAXtide560 substrate. …
April 12, 2010
The Open Vaccine Journal
Li Q, Peachman KK, Sower L, Leppla SH, Shivachandra SB, Matyas GR, Peterson JW, Alving CR, Rao M, Rao VB
Product: Anthrax Protective Antigen (PA), Recombinant from B. anthracis
Protein Purification:
Highly purified recombinant proteins were prepared in our laboratories for binding assays as well as immunizations, as described below. For ELISA and LTx neutralization assays, purified PA and LF were purchased from List Biologicals Laboratories, Inc., Campbell, CA. …
Author did not indicate which specific lethal factor was utilized. List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).
December 22, 2009
Biochemistry
Sutherland JN, Maynard JA
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
PTx (holotoxin), the full length PTx-S1 (1-235) protomer, and the B oligomer were purchased from List Biological Laboratories, Inc., Campbell, CA.
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
November 2, 2009
The Journal of Clinical Investigation
Wang L, Harrington L, Trebicka E, Shi HN, Kagan JC, Hong CC, Lin HY, Babitt JL, Cherayil BJ
Product: Unspecified List Labs LPS
Macrophage stimulation:
Thioglycollate-elicited peritoneal macrophages were prepared as previously described (25, 41) and stimulated with 100 ng/ml LPS (Ultra-pure; List Biological Laboratories), …
Author did not specify which List Labs LPS product was utilized in their research. List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/
October 1, 2009
List Labs POSTER - Presented at the 46th Annual Interagency Botulinum Research Coordinating Committee Meeting, October 2009 in Alexandria, VA.
Christian, T.; Shine, N.
Product: SNAPtide® Peptide Substrate (o-Abz/Dnp) for C. botulinum Type A Neurotoxin
Materials:
SNAPtide substrate #520 contains the FRET pair o-aminobenzoic acid/2,4 dinitrophenyl (o-Abz/Dnp) and SNAPtidesubstrate Mca/Dnp contains (7methoxy-coumarin-4-yl)acetyl/2,4 dinitrophenyl as its FRET pair. Botulinum Neurotoxin Type A (Product #130A) and the SNAPtidesubstrates are products of List Biological Laboratories.
Methods – The above materials were used in the following (refer to poster for details):
October 1, 2009
List Labs POSTER - Presented at the 46th Annual Interagency Botulinum Research Coordinating Committee Meeting, October 2009 in Alexandria, VA
Shine, N.; Endrukaite,E.; Christian, T.
Product: VAMPtide® Peptide Substrate (o-Abz/Dnp) Substrate for C. botulinum Type B Neurotoxin
Materials:
SNAP Etide550, VAMPtide540, SNYTAXtide560, and SNAPtidesubstrates 520, 521, and 522, and the Botulinum neurotoxin types A, B, C, and E light chains, recombinant (Product #610A, 620A, 625A, and 635A) are products of List Biological Laboratories, Inc.
Methods – The above materials were used in the following (refer to poster for details):
August 1, 2009
Infection and Immunity
Boyer AE, Quinn CP, Hoffmaster AR, Kozel TR, Saile E, Marston CK, Percival A, Plikaytis BD, Woolfitt AR, Gallegos M, Sabourin P, McWilliams LG, Pirkle JL, Barr JR
Product: Anthrax Lethal Factor (LF), Recombinant from B. anthracis
LF quantification:
… To achieve the necessary dynamic range and to account for high-sensitivity rapid-response sample analysis, two standard curves were prepared, one for 20 ng/ml, l (low volume [LV]) and the other for 200 ng/ml, l (high volume [HV]) serum volumes. In addition, three quality control samples (QCs) were prepared in human serum pools (Interstate Blood Bank, Memphis, TN) using recombinant LF (List Biological Laboratory, Campbell, CA) and validated. …
PA antigen capture immunoassay:
Microtiter plates (Thermo Labsystems, Franklin, MA) were coated with monoclonal anti-PA antibody AVR1046 obtained by the same methodologies used for anti-LF monoclonal described previously (4) at 1.28 g/ml in 0.01 M phosphate-buffered saline (PBS), pH 7.4. Coated plates were washed with a wash buffer consisting of PBS and 0.1% Tween 20, pH 7.4. Recombinant PA (rPA; List Biological Laboratories, Inc., Campbell, CA) was serially diluted twofold using a 1/25 or a 1/100 dilution of normal rhesus macaque serum (RMS). The rPA dilutions formed a seven-point standard curve from 0.6 to 37.5 ng/ml. …
Author did not indicate which specific lethal factor was utilized. List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).
April 20, 2009
The Journal of Comparative Neurology
Jhou TC1, Geisler S, Marinelli M, Degarmo BA, Zahm DS
Product: Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
Tracer injections
Several minutes after being given intraperitoneal injections of a cocktail, consisting of 45% ketamine (100mg/ml), 35% xylazine (20mg/ml) and 20% physiological saline at a dose of 0.16 ml/100g of body weight, rats were placed into a Kopf stereotaxic instrument. The skulls were exposed and small bore holes were created to allow selected brain structures to be targeted by filament-containing borosilicate glass pipettes (O.D. – 1.0 mm) pulled to tip diameters of 10–25 μm and containing the retrograde tracers Fluoro-Gold (FG; Fluorochrome, Inc., Englewood, CO; 1% in 0.1M cacodylate buffer, pH 7.4) or cholera toxin B subunit (CtB, List Biological Laboratories, Campbell, CA),
• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
April 10, 2009
PLoS One
Jee-Boong Lee, Ji-Eun Jang, Man Ki Song, Jun Chang
Product: Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
Reagents:
CT and CTB were purchased from List Biological Laboratories (Campbell, CA). …
Immunization:
For peptide immunization, 26106 OT-II splenocytes were i.v. injected into B6 mice, and then recipient mice were i.n. immunized with 40 mg of OVA peptide plus 30 mg of CpG ODN, 0.2 mg of CT, or 2 mg of CT or CTB in PBS. …
Cytokine assays:
Dendritic cells (DCs) were generated from bone marrow of B6 or BALB/c mice by culturing in complete RPMI medium containing 10% FBS supplemented with 10 ng/ml recombinant GM-CSF and IL-4 (R&D, Minneapolis, MN). After 7 days of culture, non-adherent cells were harvested by gentle pipetting, and DCs were enriched by density gradient centrifugation over Percoll medium. After purification, DCs were further incubated with PBS or CT (100 ng/ml) for 3 days and the culture supernatants were harvested at the indicated time points. …
• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
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