Bacterial Toxin Research Citations

… To test this hypothesis, we used immunofluorescence to determine if Y1R are present in PVN neurons that project to RVLM, identified by prior RVLM microinjection of the retrograde tracer cholera toxin subunit B (CTb, 0.25%; List Laboratories). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… For the injection of cholera toxin subunit B (CtB) into LHb, a glass micropipette with an outside tip diameter… was pulled on a Flaming-Brown micropipette puller (Sutter Instrument Co, CA, USA) and filled with 1% CtB (List Biological Laboratories, Campbell, CA, USA …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Iontophoretic injections of 0.5% CTb (List Biologicals, Campbell, CA, USA) dissolved in 0.01 M phosphate buffer (PB; pH 7.4) were done by applying a 0.5- to 1.0-A positive current (200 ms pulses at 2 Hz for 15 min) through a chlorinated silver wire placed in a glass pipette …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Drugs and treatments:

… For pertussis toxin (PTx) experiments, cells were pretreated with PTx (List Biological Laboratories, Inc.) at a final concentration of 200 ng/ml or vehicle (in serum-free DMEM) for 24 hours, and PTx was maintained during drug stimulation. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animals and Drug Treatments:

Diphtheria toxin (DT; List Biological Laboratories) was administered intraperitoneally at 15 ng/g, on 2 consecutive days.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

To examine the time-course of axonal renervation, a cholera toxin B subunit (CTB, 5 µg/mouse, List Biol Lab) was injected into the tongue under deep anesthesia three days before fixation. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Immunohistochemistry:

… Finally, sections were incubated in 3,3 -diaminobenzidine (DAB, Sigma) and nickel ammonium sulfate, producing a purple-black reaction product in the nucleus. Subsequently, CTb was visualized by incubating the same tissue sections in goat anti-CTb (1:20,000, List Biological Laboratories) overnight, and …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Simultaneously with rMOG as well as 48 h later, each mouse received intraperitoneally 200 µL Pertussis Toxin (PTX) (1 µg/mL in PBS) (List Biological Laboratories, Inc., CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Antibodies and Reagents:

… Clostridium difficile toxin B was obtained from List Biological Laboratories (Campbell, CA). …

Rho Family GTPases Are Required for Efficient Transport of Influenza A Virus NA to Cell Surface:

… Next, we used this established system to investigate the contribution of Rho GTPases to influenza virus NA transport to the cell surface. Rho-GTPase activity was acutely inhibited in cells treated with C. difficile toxin B, as toxin B glucosylates all members of the Rho family and inhibits their effector interactions (30, 40,,42). …
To further confirm these observations, we used an independent approach to test the effects of toxin B on NA transport to the plasma membranes. …

• Product #155A – Toxin B from Clostridium difficile

Induction of EAE in Mice by Immunization with MOG and Adjuvant and Treatment with HspB5:

… EAE was induced in female C57BL/6J mice (The Jackson Laboratory, Sacramento, CA) at 9 weeks of age by subcutaneous immunization in the flank with an emulsion containing 200 µg of myelin oligodendrocyte glycoprotein3555 (MOG3555; MEVGWYRSPFSRVVHLYRNGK) in saline and an equal volume of complete Freund’s adjuvant containing 4 mg/ml of mycobacterium tuberculosis H37RA (Difco Laboratories, Detroit, MI). All mice were administered 400 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) intraperitoneally at 0 and 48 h after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)