Bacterial Toxin Research Citations

Mice and immunizations with the YopE69-77 peptide:

Female C57BL/6J mice (Jackson Laboratory) were used for all experiments. Animals that were 9 to 10 weeks old were anesthetized with a mixture of ketamine (60 mg/kg of body weight) and xylazine (10 mg/kg) delivered by intraperitoneal (i.p.) injection. A mixture of 1 µg of cholera toxin (CT; List Biological Laboratories Inc., Campbell, CA) … Control mice received CT in 15 µl of PBS only. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… The mice were orally sensitized twice per week for 4 weeks with 1 mg of Ovm (19) adjuvanted with 10 µg of cholera toxin (CT; List Biologicals, Campbell, CA, USA) (8), as outlined in Fig. 1. The negative control group received CT alone (n = 10). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… These mononuclear cells were stimulated by 100 ng/ml LPS (Salmonella minnesota R595 ultra pure LPS; List Biological Laboratories, Campbell, CA) and sampled at 0 (before stimulation), 2, 4, 8, and 24hr. …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

Reagents:

… An ultrapurified form of E. coli 0111:B4 LPS was purchased from List Biological Lab. Inc. (Campbell, CA). …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

… Ultra pure LPS from Escherichia coli 0111;B4 was obtained from List Biological Laboratories (CA). …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Treatment of reproductive tract cells with PAMP:

To determine whether PAMP affected antigen presentation by uterine and vaginal cells, PAMP were added to individual wells in antigen presentation assays at the start of each experiment. To measure the effect of PAMP on co-stimulatory molecule expression, PAMP were added to epithelial cells, stromal cells and vaginal cells (5 10⁵ cells/100 ml) at the start of each 24 hr incubation period as previously described.^28,44 The TLR agonists used in these studies included TLR1/TLR2: Pam₃Cys-Ser-(Lys)4 (P3C, EMC Microcollections, Tbingen, Germany) at a final concentration of 10 g/ml; TLR2/TLR6: Peptidoglycan from Staphylococcus aureus (InvivoGen), 10 g/ml; TLR3: Poly(I:C) (InvivoGen), 25 g/ml; TLR4: ultra-pure lipopolysaccharide from Salmonella minnesota (LPS; List Biological Laboratories, Inc., Campell, CA), … Wells containing media alone were included in all experiments as controls. The TLR agonists were tested for LPS contamination using the Limulus assay …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Antibodies and reagents:

… LPS (List Biological Laboratories), … were used to stimulate BMDCs.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Reagents:

LPS were from Escherichia coli O111:B4 (List Biological Laboratories, Campbell, CA, USA), …

Results – MLK4 does not interact with Cdc42, TRAF2 or TRAF6:

… TRAF6 is an effector protein recruited to the TLR4 receptor complex after cells are stimulated with LPS. …

• Product #201 – LPS from Escherichia coli O111:B4

Antigens:

Two of the PT antigen … obtained from commercial sources, List Biological Laboratories Inc. (List) (Campbell, CA) …

PT was utilized in:  Coating optimization testing for PT antigens

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… supplemented with 3 mg/ml M. tuberculosis H37Ra (BD Difco, Detroit, MI; 400 µg/mice). Mice were injected with 400 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) via a tail vein at the same time of immunization and again 48 hrs later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)