Bacterial Toxin Research Citations

In vivo experimental human endotoxaemia model and ex vivo analyses

Eight healthy (as confirmed by medical history, physical examination and routine laboratory testing) male volunteers with ages ranging from 18 years to 35 years provided written informed consent to participate in experimental endotoxaemia experiments conducted at the research unit of the intensive care department of the Radboud University Medical Center. …

A continuous endotoxin infusion regimen was used, as is described in detail elsewhere40. In short, participants were admitted to the research unit, and an antecubital vein and radial artery were cannulated to allow administration of fluids and endotoxin, and blood sampling and haemodynamic monitoring, respectively. A 3-lead ECG was recorded continuously throughout the experiment. After iso-osmolar pre-hydration (1.5 l NaCl 0.45% and glucose 2.5% administered intravenously in the hour before the start of endotoxin infusion), volunteers were intravenously challenged with a loading dose of 1 ng kg−1 bodyweight endotoxin (Escherichia coli lipopolysaccharide (LPS) type O113, lot no. 94332B1; List Biological Laboratories), directly followed by continuous infusion of 0.5 ng kg−1 h−1 for 3 h. Participants were monitored for 8 h after the endotoxin loading dose after which they were discharged from the research unit. …

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113

… Vero cells treated with purified C. difficile toxins (A and B) and antitoxin (List Biological Labs, 152C and 155C; TechLabs, T5000) were used as controls. …

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

… Anthrax protective antigen (PA) was obtained from List Biological Laboratories List Labs), and stored at 4°C. …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Depletion of AMs was induced by intranasal instillation of 15ng/mouse of diphtheria toxin (DT, List Biological Laboratories) with double administration three days apart …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… In addition, pertussis toxin (200 ng; List Biological Laboratories) was injected intravenously on days 0 and 2. The severity of EAE was monitored and graded in a blinded …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.2 Induction of EAE and treatment

EAE was induced in 8- to 12-week-old mice with a s.c. injection of 150 µg myelin oligodendrocyte glycoprotein-derived 35-55 peptide (MOG35-55, MEVGWYRSPFSRVVHLYRNGK, CPC Scientific, California, US), emulsified in Incomplete Freund’s adjuvant containing 500 µg Mycobacterium tuberculosis (BD Difco, Detroit, Michigan, US) followed by an i.p. injection with 500 ng Bordetella pertussis toxin (List Biological, Campbell, California, US) on the day of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

T-cell chemotaxis

… Human T cells were incubated with 200 nM CCR6 antagonist (CCX2553, ChemoCentryx, USA) or vehicle control (DMSO) for 20 min prior to the chemotaxis assay. Preincubation of human T cells with 50 ng/mL of pertussis toxin (PTX) (List Biological Laboratories, Campbell, CA, USA) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice received i.p. injections with 200 ng of Bordetella pertussis toxin (List Biological Laboratories) day 0 and day 2 postimmunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Diphtheria toxin, VEGF, PD-1, CCR8 antibody and CCR2i treatments

DT (List Biological Laboratories) was administered to mice (1 μg per mouse in PBS) via retro-orbital injection twice on two consecutive days. For tumor transplantation experiments, DT was injected on days 8 and 9 after tumor s.c. transplantation. Mouse polyclonal neutralizing VEGF-A antibody (R&D clone AF-493-M) or control IgG (BioXcell clone BE0130) were injected on days 9, 12 and 15 (20 μg per mouse per injection) with or without DT, or on days 8, 10, 12, 14 and 17 with or without CCR8 antibody (BioLegend clone SA214G2; 240 μg per mouse per injection). …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… female B6 mice were subcutaneously immunized with 150 µg human IRBP1-20 emulsified with CFA at the hind foot pad and at six points on the back. Before the injection, mice received 300 ng pertussis toxin (List Labs, USA) intraperitoneally. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)