Bacterial Toxin Research Citations

EAE

Active immunization was induced by s.c. injection of MOG[35-55]11 (CHU de Québec) in incomplete Freund’s adjuvant (Difco) supplemented with 5 mg mL-1 Mycobacterium tuberculosis extract (Fisher). A dose of 25 μg MOG[35-55] per mouse was used to compare WT to Serpine1-KO mice, while 100 μg per mouse was used in EAE experiments involving Tim-3-Tg mice. In Figure 1D, passive EAE was induced by first immunizing Serpine1-WT 2D2 and Serpine1-KO 2D2 mice with MOG[35-55]. Nine days later, splenocytes were collected and stimulated ex vivo for 48 hours with MOG[35-55] (20 μg mL-1), rmIL-12 and rmIL-23. Next, 20×106 splenocytes were injected i.p. into unimmunized B6 recipients. Adoptive transfer of WT 2D2, Serpine1-KO:2D2 or RV-transduced 2D2 Th1 cells entailed i.v. injection (2×106) into Rag1-/- recipients at d5 of culture. In all EAE experiments, mice received 200 ng pertussis toxin (List Biological Labs) i.p. at d0 and d2. Mice were assessed for clinical symptoms daily as previously described (9).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction

Age- and sex-matched mice were immunized with 100 ug MOG35-55 peptide (MOGp35-55) in complete Freund’s adjuvant (Sigma-Aldrich) in the rear flank s.c. The mice were further challenged with 250 ng of pertussis toxin (List Biological) on day 0 and day 2. Paralysis in mice were monitored and scored as previously described criteria.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Allergic sensitization and challenges

… For oral sensitization experiments, mice were given oral gavages on days 0 and 7 with 5 mg of grade III OVA with 0.5 mg kg−1 of cholera toxin (List Biologicals 100B, lot nos. 10165A1 and 10165A2) in a final volume of 0.25 ml of 0.2 M sodium bicarbonate buffer. Both sensitization and challenge were carried out around zeitgeber time 4 (ZT4).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

2.1. Reagents

… LPS (Escherichia coli 0111:B4) from List Biological Laboratories (Campbell, CA, USA), …

• Product #201 – LPS from Escherichia coli O111:B4

… and LPS from E. coli O55:B5 was purchased from List Biological Laboratories for in vivo studies …

• Product #203A – LPS from Escherichia coli O55:B5

… CTB (Choleragenoid, 103B) and CT (100B) were purchased from List Biological Laboratories. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… At the times of immunization and 2 d later, mice were iv injected with 400 ng of pertussis toxin (List Biological Laboratories). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

OVA/cholera toxin allergy model

OVA grade III (A5378; Sigma-Aldrich) was provided at 0.1% in drinking water, autoclaved for sterility for 3 d (Feeding, Tolerance) or not (Allergy) to initialize tolerance. All mice were then provided with regular drinking water for 1 wk. 1 mg OVA in 0.2 M sodium bicarbonate (Feeding) or 1 mg OVA + 20 µg cholera toxin (100B; List Biological) in 0.2 M sodium bicarbonate (Tolerance, Allergy) were provided once per week for 3 wk, followed by endpoint analysis 2 d after the final dose. Serum was harvested for ELISA 1 d prior to endpoint analysis. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Pertussis toxin (180) was purchased from List Biological Laboratories (Campbell, CA, US). …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer