Bacterial Toxin Research Citations

… Pertussis toxin List Biologicals Laboratories (Campbell, CA) Cat# 181 …

Induction of active EAE

Active EAE was induced by subcutaneous (s.c.) injection of MOG35-55 (MOG) peptide (200 µg/mouse) emulsified in complete Freund’s adjuvant (CFA) containing Mycobacterium tuberculosis H37Ra (5 mg/ml) at the flanks of mice. Pertussis toxin (200 ng/mouse) was given intravenously (i.v.) on days 0 and 2. The mice were observed daily for the development of clinical symptoms, and mice were clinically scored.

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Materials and Instruments

… Botulinum toxin type A heavy chain (BTX/A/Hc) was obtained from List Biological Laboratories, Inc. (Campbell, CA, USA) under regulations of US Department of Commerce, and BTX/A/Hc was used under the Civil Protection Law in Japan. …

LSPR Detection of Biological Toxins

Using the biological toxins (BTX/C or BTX/A/Hc) and the glycolipid chips functionalized with double layers having different Au nanoparticle sizes (20–80 nm), detection responses were examined by our LSPR method as previously reported. (14,16) The LSPR system is composed of a tungsten halogen lamp as a light source, a portable pump for flowing, an injection valve with a sample loop for 1 mL, a disassemble flow cell (optical length, 0.025 mm) for attachment of the prepared chips, a temperature-control sample compartment with a Peltier-controlled cuvette holder, a spectrometer for detection of absorbance changes and a PC for data analysis. The running buffer used in the experiments was 10 mM HEPES (pH 7.5) containing 150 mM NaCl, filtered with a 0.22 μm filter, and degassed before use through all the LSPR experiments. The LSPR system detects and measures absorbance changes when the analytes (the samples being analyzed) start to bind and dissociate from the chip surfaces. The buffer was run in the LSPR system until the baseline was made stable. Each 1 mL of BTX/C (50 ng/mL) or BTX/A/Hc (5, 25, and 50 ng/mL) was injected for 15 min at a flow rate of 66.6 μL/min. As the negative control, RCA120 (100 ng/mL) was also injected. The temperature was 25 °C. The integration time was 8 ms, and the number of accumulation times was 2000. LSPR responses were monitored at A550 – A720 with time. All LSPR data were analyzed with OOIBase32 software (Ocean Optics, version 2.0).

• Product #612A – Botulinum Neurotoxin Type A Heavy Chain, Recombinant, Binding Domain

… All mice were then provided regular drinking water for 1 week. 1 mg OVA in 0.2M sodium bicarbonate (Feeding), or 1 mg OVA + 20 ug cholera toxin (List Biological; 100B) in 0.2M sodium bicarbonate (Tolerance, Allergy), were provided once per week for 3 weeks, followed by endpoint analysis 2 days after the final dose …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… For double immunofluorescence labeling of CTb and Fos, sections were incubated overnight 292 with an anti-CTb goat serum (1:4,000; #703, List Biological Labs) and an anti-Fos rabbit serum 293 (1:10,000; PC38, Merck Millipore). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

PT-specific lymphocytes and cytokine assessment

At the respective timepoints, additional heparin tubes (12mL) were collected from children that were conveniently selected for assessment of their CMI responses against PT. CD3+ CD3+ CD4+ and CD3+CD8+ PT-specific lymphoblasts were measured using a specialized Flowcytometric Assay that detects the Specific CMI response in Activated whole blood(FASCIA). Similar to previous protocols [11, 16-18], whole blood was diluted 1/10 in complete RPMI medium (supplemented with 50 μg/mL gentamycine, 2 mM L-glutamine, MEM non84 essential AA, Na pyruvate, 2B-mercapthoethanol and Fetal Bovine Serum) and stimulated with 5 μg/mL PT antigen (heat-inactivated at 96°C for 20 minutes, Bordetella pertussis strain (native toxin), List Biological.