Citations

… Female mice were sensitized on days 0 and 7 by oral administration (p.o.) in 250 µL PBS containing 1 mg of casein (Sigma-Aldrich, St. Louis, MO) and cholera toxin 10 µg (CT, List Biological Laboratories, Inc., Campbell, CA) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Additionally, the mice received 200 ng of pertussis toxin (List Biological Laboratories) ip on days 0 and 2 after immunization. Mice were monitored daily for EAE using the following scoring system: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Mice were immunized subcutaneously in two sites on either side of the midline on the lower back, with 200 µg MOG35-55 in 0.2 ml emulsion. Pertussis toxin (200 ng, List Biological Laboratories, Campbell, CA) was administered intraperitoneally (i.p.) on the day of MOG35-55 immunization and 48 h later …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For cholera toxin responses, mice were immunized with 10 ug of cholera toxin (List Biological Laboratories) in PBS by oral gavage. Animals received cholera toxin every 7 days for three consecutive weeks. Mice were analyzed 7 days after last immunization …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Pertussis toxin (200 ng/mouse, List Biological) was injected intraperitoneally on day 0 and 2. A total of 1 × 106 live CD4+T cells were transferred into …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… pressure or iontophoretic injections were used to deliver CTb (List Biological Laboratories, Campbell CA, Cat# 104) into primary visual cortex after reconstitution in 0.1M potassium phosphate buffer (PB, 1%, pH 6.0) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE immunizations and scoring

8-10-week-old mice were immunized for EAE as previously described [8] with minor modifications. Briefly, mice were administered murine MOG 35-55 (Johns Hopkins Peptide Synthesis Core Facility, Baltimore) in incomplete Freund’s adjuvant (Sigma-Aldrich) supplemented with heat-killed mycobacterium tuberculosis (Sigma-Aldrich) via two subcutaneous flank injections. On days 0 and 2, mice received an intraperitoneal injection of 250 ng pertussis toxin (List Biological Laboratories). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… LPS (Escherichia coli O113) is obtained from List Biological Labs, Inc., Campbell, CA, USA …

• Product #433 – LIST™ HPT™ from Escherichia coli O113

4.4. Experimental autoimmune encephalomyelitis (EAE)

To induce a chronic form of EAE, mice were subjected to a protocol in which MOG35–55, a fragment of myelin oligodendrocyte glycoprotein, is twice administered peripherally in adjuvant (Constantinescu et al., 2011). 200 μg of MOG35–55 in a 1:1 emulsion of phosphate-buffered saline (PBS) and Difco Complete Freund’s Adjuvant (BD, Franklin Lakes, NJ, USA) supplemented with an additional 100 mg of Difco Mycobacterium tuberculosis H37RA (BD, Franklin Lakes, NJ, USA) was split into two equal portions and subcutaneously injected in the left and right flanks posterior to the forelimbs. This initial injection was specified as day 0 of EAE. This protocol results in the expansion of T cells autoreactive to myelin. Additionally, the mice received a peritoneal injection of 300 ng of pertussis toxin (List Biological Laboratories, Campbell, CA, USA) dissolved in PBS. Pertussis toxin causes permeabilization of the blood brain barrier, thus allowing the T cells targeting MOG to enter the CNS. After two days, the mice were given an additional 300 ng booster injection of pertussis toxin to maintain permeabilization of the blood brain barrier. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Pertussis toxin List Biological Laboratories Cat# 180

Treatment of experimental autoimmune uveitis

Starting two weeks before immunization, B10.RIII mice were fed folate deficient diet and continued for a total of 24 days until euthanasia. To induce EAU, the animals were actively immunized on day 0 with a single subcutaneous injection of 50 μg of human interphotoreceptor retinoid-binding protein (IRBP) peptide 161-180 (IRBP161-180, SGIPYIISYLHPGNTILHVD; GenScript, cat.# RP20268), emulsified in complete Freund’s adjuvant (CFA) containing 2.5 mg/mL of Mycobacterium tuberculosis (BD Difco™). Pertussis toxin (List Biological Laboratories, Campbell, CA), a Th1-biased adjuvant, was given as 2 × 200 ng intraperitoneal doses on days 0 and 2 to enhance organ-specific autoimmunity. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer