Bacterial Toxin Research Citations

… For anti-filamentous hemagglutinin (FHA) or anti-pertactin (PRN) ELISAs, CoStar 96 well high-binding
plates (Corning, Corning, NY) were coated with either 2 µg/ml FHA (BP-FHA-100, The Native Antigen
Company/Cedarlane, Burlington, NC) or 1 µg/ml PRN (B. pertussis Pertactin, Strain: B. pertussis strain
165, The List Labs, St Campbell, CA) in carbonate buffer pH 9.6, incubated overnight at 4°C, washed 3x
with wash buffer (KPL 10X Phosphate Buffered Saline with 0.05% Tween 20 (Fisher Scientific)) and
blocked with Superblock (ScyTek) for 1 h at room temperature (RT). …

• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

2.2. Antigens Vibrio cholerae toxin (CT holotoxin; lot 10154B5A; Mr 84,000) was from List Biological Labs (Campbell CA, USA) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cell culture

… Where indicated, cells were also treated with nigericin (5 μM, Invivogen, 10–45 min), CTB (20 μg ml−1, List Biological Laboratories, 6 h), silica (200 μg ml−1, Invivogen, 6 h), TcdB (10 μg ml−1, R&D Systems, 8 h) and ATP (5 mM, Millipore-Sigma, 25 min); cultured in K+-free medium (0.8 mM MgCl2, 1.5 mM CaCl2, 10 mM HEPES, 5 mM glucose and 140 mM NaCl, pH 7.2, 3 h)50; or transfected with flagellin (500 ng ml−1, Invivogen, 4 h), poly(dA:dT) (1 μg ml−1, Invivogen, 4 h) or FSL-1 (0.2 μg ml−1, Invivogen, 4 h) and ultrapure LPS (1 μg ml−1, 4 h) with Lipofectamine 2000 (Invitrogen) or as otherwise indicated.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Stimuli: LPS O111:B4 (L3012, sigma), LPS O55:B5 (L2637, sigma), CTB (103B, List Biological Laboratories), …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… XCR1+ cDC1 cells were depleted by injecting Xcr1DTR mice with 500ng un-nicked DT (150, List Biologicals) intraperitoneally in 100µl PBS. DT was typically administered 2d before induction of MICB expression on tumour cells by doxycycline treatment and then every 48h until the study endpoint. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… For oral vaccinations, 10µg of cholera toxin (List Biological Laboratories #100B) was administered by gavage weekly. Serum was collected under isoflurane anesthesia, by retro-orbital puncture. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Diphtheria toxin (DT) and puromycin treatments in vitro

Transfected HEK293 cells for human diphtheria toxin receptor (hDTR) mutagenesis were selected with 20 ng/ml DT (List Labs, #150) from day 3 after electro-transfections. Cell clones mutated in hDTR-sgRNA-1 or hDTR-sgRNA-2 targeted regions were cultured with DT-supplemented growth medium from day 1 after cell sorting (day 0 refers to the day of cell sorting). Transfected PFFs were selected with 300 ng/ml puromycin-containing (MPBIO, 219453925) medium for 48 h after electro-transfections of 12 h. Both DT and puromycin-supplemented growth medium were exchanged daily until negative control cells died completely.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

EAE induction.

EAE was induced by subcutaneous immunization of mice into the flanks with an emulsion of MOG35-55 peptide (150 μg) emulsified in complete Freund adjuvant supplemented with 4mg/ml of M. tuberculosis extract H37Ra (Difco). In addition, the animals received 200 ng of pertussis toxin (List Biological Laboratories) i.p. on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Whey Sensitization/Challenge Protocol and Assessment of Anaphylaxis

The model of cow’s mike allergy-induced autism was established as described by de Theije’s et al, with modifications.37 Whey is a milk protein fraction composed of several milk allergens (β-lactoglobulin, α-lactalbumin, bovine lactoferrin, bovine serum albumin, and bovine immunoglobulins), some of which are major allergens, and used to induce our food allergy model.38 After 1 week acclimation, mice were sensitized intragastrically (i.g) with 20 mg whey (Hefei Bomei Biotechnology Co., LTD, China) in 0.5 mL of PBS plus 10 μg cholera toxin (CT, List Biological Laboratories, Campbell, CA, USA) as an adjuvant from week 1 through week 5 and oral 100 mg whey containing 10 μg CT from week 6 through week 7. Mice were then sensitized via intraperitoneal injection (i.p.) with 500 μg whey in 0.2 mL of PBS containing 2 mg Alum (ThermoFisher, USA) from week 8 through week 9. Naive mice received PBS alone. Sham mice received CT and alum alone. A schematic for the sensitization and challenge timeline was depicted in Figure 1. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… For macrophage ablation, CD11b-DTR mice received a tail vein injection of 5µg/kg diphtheria toxin (DT, cat. #150, List Bio, Campbell, CA) …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae