Bacterial Toxin Research Citations

Reagents

…Recombinant PA was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA) …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Then all mice were treated twice with 200 ng pertussis toxin (List Biological Laboratories, USA) in total by intraperitoneal injection at 0 and 48 h after immunization …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis (EAE) models

MOG35-55 peptide was used to immunize 12 female C57Bl/6J mice with a dose of 150 μg (Peptide international)mixed with complete Freud’s adjuvant (CFA; SigmaAldrich; USA) in concentration of 10 mg/ml heat-killed Mycobacterium tuberculosis H37Ra (Difco Laboratories). Pertussis toxin were injected into mice intraperitoneally (List Biological Laboratories; 500 ng) at day 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

STAR★Methods
Key resources table

Lipopolysaccharide, e-coli-O11:B4-derived (LPS) List Labs #201

Murine primary cell cultures

Peritoneal cells were isolated from naïve mice by lavage with 10mL of PBS containing 0.5mM EDTA. Lavage was estimated to contain 50% macrophages. Peritoneal macrophages were isolated by adhesion purification of lavage cells in RPMI-10 (RPMI-1640 containing 10% heat-inactivated fetal bovine serum (v/v), 1X Pen/Strep/Glut, 1mM HEPES, and 100μM Sodium Pyruvate) plated 1.3×106 macrophages/well in 6-well plates for FACS sorting or 4×106 macrophage/well in 24-well plates for flow cytometry. Non-adherent cells were removed by washing after 3-5 hours, and macrophages were cultured in fresh RPMI-10 overnight. 400ng/mL purified E. coli O111:B4 LPS (List Biological Laboratories Inc.) was added to generate inflammatory macrophages 12h before the addition of target cells. …

• Product #201 – LPS from Escherichia coli O111:B4

4.1 | Reagent

… Purified LPS was purchased from List Biological Lab …

4.6 | Western blot analysis

Western blots were performed essentially in the same manner as previously described.[22] Briefly, cells (0.5 × 106 cells/ml) were seeded into six‐well plates, incubated for 48 h, and pretreated with different concentrations (50 and 100 nM) of EXM for 1 h. These cells were stimulated with LPS (10 ng/ml) or poly[I:C] (10 μg/ml) for 18 h, col- lected, and washed with ice‐cold phosphate‐buffered saline (PBS). …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

… Additionally, mice received 400 ng pertussis toxin (PTX) (List Biological Laboratories) intraperitoneally (i.p.) on days 0 and 2 after immunization …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… goat anti-cholera toxin B (CTB, 1:1000, List Labs …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Pertussis Toxin (PTX) List Biological Laboratories, Kalifornien (USA) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For induction of active EAE, C57BL/6 mice were immunized s.c. at the tail basis with 50 µg MOG35-55 peptide (GenScript) emulsified in Complete Freunds adjuvant (CFA, BD) along with 100 ng pertussis toxin (PTX, List-BiologicalLaboratories) administration i.p. on day 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin (List Biological Lab, Kat. Nr. #181, Lot Nr. 181236A1) …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)