Bacterial Toxin Research Citations

… At T=0, 1ng/kg bodyweight LPS (E. coli type O113, Lot no. 94332B1; List Biological Laboratories, Campbell, USA) was administered intravenously to elicit a transient systemic inflammatory response …

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113

…Whole cholera toxin (List Biological Laboratories, Campbell, CA) was prepared in carbonate-buffered saline (Lycke et al., 1999) the day of immunization and administered by gavage every seven days …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… On study days 14, 21, 28, 35 and 42 mice were orally sensitized via a gavage with 20 mg of homogenized whey (WPC60, Milei, Friesland Campina) dissolved in 0.5 mL phosphate-buffered saline (PBS) containing 10 µg cholera toxin (CT; List Biological Laboratories) as an adjuvant …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Anthrax lethal factor and anthrax protective antigen were obtained from List Biologicals. Cells were treated for two hours at 37°C in 5% CO2 …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

IN VIVO EXPERIMENTAL HUMAN ENDOTOXEMIA

Human endotoxemia experiments were conducted at the research unit of the Intensive
Care department of the Radboud university medical center according to our standard
protocol (21). Subjects refrained from caffeine and alcohol in the 24 h before the
experiment and from food and drinks 10 h before the experiment. One venous canulae
was placed in the antecubital vein, for fluid infusion and endotoxin administration.
Subjects were pre-hydrated in the hour before LPS administration (1.5 L 2.5%
glucose/0.45% saline) followed by 150 mL/h for 6 h (until the end of the experiment).
Blood pressure monitoring and blood withdrawals were performed using an arterial
cannula placed in the radial artery. In addition, ECG was continuously monitored and
vital signs were recorded every 5 seconds on a Phillips MP50 patient monitor using an
in-house developed data capturing system. Body temperature was measured every 30
mins with infrared tympanic measurements (FirstTemp Genius 2, Covidien Geldermalsen,
the Netherlands). At T=0, 1 ng/kg bodyweight LPS (E. coli type O113, Lot no. 94332B1;
List Biological Laboratories, Campbell, USA) was administered intravenously to elicit a
transient systemic inflammatory response.

• Product #9433 – GMP LPS Lipopolysaccharide List™ Hpt™ From Escherichia Coli Type O113

… Pertussis toxin List Biological Laboratories, Hornby, ONT, Kanada; Cat # 181 …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… To facilitate lymphocyte transfer to the CNS, 300 ng of pertussis toxin (List Biologicals, Campbell, USA) was administered intraperitoneally two hours and two days after EAE induction (Contarini et al., 2018). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… rPA83 B. anthracis WB (40 ng) c = 1 mg/ml; List Biologicals, Campbell, USA …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… DT powder is purchased from Sigma-Aldrich #D0564 or List Biological Labs #150 and can be stored at 2-8 °C …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Cell culture and treatment with S100A4

BMDCs were generated by culturing bone marrow cells in culture medium (RPMI-1640; Sigma-Aldrich) supplemented with 10% fetal bovine serum (Gibco), 1% L-glutamine, 1% gentamicin, and 50 μM 2-mercaptoethanol (all from Sigma-Aldrich) in the presence of 200 ng/ml recombinant Flt3L (PeproTech) for nine days. Cells were incubated in vitro with various stimuli, including CT (List Biological Laboratories) (0.1 μg/ml), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae