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Methods In Molecular Biology
Cherubin, P;Quiones, B;Elkahoui, S;Yokoyama, W;Teter, K;
Many AB toxins elicit a cytotoxic effect involving the inhibition of protein synthesis. In this chapter, we describe a simple cell-based fluorescent assay to detect and quantify the inhibition of protein synthesis. The assay can also identify and characterize toxin inhibitors.