Citation

Stimulation of phospholipase C (PLC) by the activated -subunit of Gq (Gq) constitutes a major signaling pathway for cellular regulation, and structural studies have recently revealed the molecular interactions between PLC and Gq. Yet, most of the PLC-interacting residues identified on Gq are not unique to members of the Gq family. Molecular modeling predicts that the core PLC-interacting residues located on the switch regions of Gq are similarly positioned in Gz which does not stimulate PLC. Using wild-type and constitutively active chimeras constructed between Gz and G14, a member of the Gq family, we examined if the PLC-interacting residues identified in Gq are indeed essential.