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Experimental applications of TNF-reporter mice with far-red fluorescent label

Shebzukhov, YV;Kuchmiy, AA;Kruglov, AA;Zipp, F;Siffrin, V;Nedospasov, SA;

This chapter provides protocols for in vitro and in vivo analysis of TNF-producing cells from a novel TNF reporter mouse. In these transgenic mice, genetic sequence encoding far-red reporter protein Katyushka (FRFPK) was placed under control of the same regulatory elements as TNF, thus providing the basis for detection, isolation, and visualization of TNF-producing cells.