Citation

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Inflammatory monocytes orchestrate innate antifungal immunity in the lung

Espinosa, V;Jhingran, A;Dutta, O;Kasahara, S;Donnelly, R;Du, P;Rosenfeld, J;Leiner, I;Chen, CC;Ron, Y;Hohl, TM;Rivera, A;

Aspergillus fumigatus is an environmental fungus that causes invasive aspergillosis (IA) in immunocompromised patients. Although -CC-chemokine receptor-2 (CCR2) and Ly6C-expressing inflammatory monocytes (CCR2Mo) and their derivatives initiate adaptive pulmonary immune responses, their role in coordinating innate immune responses in the lung remain poorly defined. Using conditional and antibody-mediated cell ablation strategies, we found that CCR2Mo and monocyte-derived dendritic cells (Mo-DCs) are essential for innate defense against inhaled conidia. By harnessing fluorescent Aspergillus reporter (FLARE) conidia that report fungal cell association and viability in vivo, we identify two mechanisms by which CCR2Mo and Mo-DCs exert innate antifungal activity. First, CCR2Mo and Mo-DCs condition the lung inflammatory milieu to augment neutrophil conidiacidal activity. Second, conidial uptake by CCR2Mo temporally coincided with their differentiation into Mo-DCs, a process that resulted in direct conidial killing. Our findings illustrate both indirect and direct functions for CCR2Mo and their derivatives in innate antifungal immunity in the lung.