4749 total record number 121 records this year

MafB-restricted local monocyte proliferation precedes lung interstitial macrophage differentiation

Vanneste, D;Bai, Q;Hasan, S;Peng, W;Pirottin, D;Schyns, J;Maréchal, P;Ruscitti, C;Meunier, M;Liu, Z;Legrand, C;Fievez, L;Ginhoux, F;Radermecker, C;Bureau, F;Marichal, T;

Resident tissue macrophages (RTMs) are differentiated immune cells that populate distinct niches and exert important tissue-supportive functions. RTM maintenance is thought to rely either on differentiation from monocytes or on RTM self-renewal. Here, we used a mouse model of inducible lung interstitial macrophage (IM) niche depletion and refilling to investigate the development of IMs in vivo. Using time-course single-cell RNA-sequencing analyses, bone marrow chimeras and gene targeting, we found that engrafted Ly6C+ classical monocytes proliferated locally in a Csf1 receptor-dependent manner before differentiating into IMs. The transition from monocyte proliferation toward IM subset specification was controlled by the transcription factor MafB, while c-Maf specifically regulated the identity of the CD206+ IM subset. Our data provide evidence that, in the mononuclear phagocyte system, the ability to proliferate is not merely restricted to myeloid progenitor cells and mature RTMs but is also a tightly regulated capability of monocytes developing into RTMs in vivo.