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New High Affinity Antibodies Against Botulinum Neurotoxin Type A

Christian, T.; Shine, N.; Crawford, K.

New high affinity antibodies against Botulinum Neurotoxin Type A have been developed in chickens. Production of polyclonal IgY is both easy and cost effective. A single egg contains as much antibody as a single bleed from a rabbit and is a simple and less invasive production method. Eggs from immunized chickens represent a continual source of polyclonal antibody. The antigen used to challenge the chicken was a recombinant Heavy Chain Binding domain fragment (HccA) from Type A toxin. IgY derived from eggs were affinity purified using an antigen-coated column. To test the affinity purified antibodies we determined the titer of the anti-HccA antibodies against Botulinum Neurotoxin Type A (BTA) holotoxin, List Product #130. The ability of the anti-HccA antibodies to capture BTA was also tested using a Sandwich ELISA.