Citation

Conventional drug screening platforms face limitations such as single cell line analysis, slow processing, unstable fluid flow, and frequent gradient adjustments, highlighting the requirement for high-throughput alternatives. In this study, a breast cancer-on-a-chip platform integrated with a concentration gradient generator was introduced, enabling rapid and reproducible anticancer drug evaluations. Computational fluid dynamics (CFD) ensured stable gradient generation through convection and diffusion mechanisms. The platform successfully mimicked a triple-negative breast cancer (TNBC) microenvironment by co-culturing MDA-MB-231 and MCF-10A cell lines within the chip. High-throughput screening of doxorubicin (DOX) was conducted, demonstrating 82% survival of MCF-10A cells and 50% death of MDA-MB-231 cells at 18.25 µM DOX. Therefore, the newly designed platform offers precise evaluation of drug efficacy under physiologically relevant conditions and provides valuable insights into effective anticancer drug concentrations for clinical applications.