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Prospective isolation of non-hematopoietic cells of the niche and their differential molecular interactions with HSCs

Mende, N;Jolly, A;Percin, GI;Gnther, M;Rostovskaya, M;Krishnan, SM;Oostendorp, RAJ;Dahl, A;Anastassiadis, K;Hfer, T;Waskow, C;

A major limitation preventing in vivo modulation of hematopoietic stem cells (HSCs) is the incomplete understanding of the cellular and molecular support of the microenvironment in regulating HSC fate decisions. Consequently, murine HSCs cannot be generated, maintained or expanded in culture over extended periods of time. A significantly improved understanding of the bone marrow niche environment and its molecular interactions with HSCs is pivotal to overcome this challenge. We here prospectively isolated all major non-hematopoietic cellular niche components and cross-correlate them in detail with niche cells defined by lineage marking or tracing. Compiling an extensive database of soluble and membrane-bound ligand-receptor interactions, we developed a computational method to infer potential cell-to-cell interactions based on transcriptome data of sorter-purified niche cells and hematopoietic stem and progenitor cell subpopulations. Thus we establish a compendium of the molecular communication between defined niche components and HSCs. Our analysis suggests an important role for cytokine antagonists in the regulation of HSCs functions. Copyright 2019 American Society of Hematology.